儿童与成人弥漫大B细胞淋巴瘤的Hans、Choi免疫分型和c-myc基因特征

基础医学与临床 ›› 2013, Vol. 33 ›› Issue (8): 1038-1042.

儿童与成人弥漫大B细胞淋巴瘤的Hans、Choi免疫分型和c-myc基因特征

徐红艳¹,黄慧¹,杨文萍¹,黄传生²,钟梅慧¹

1. 江西省儿童医院
2. 江西省肿瘤医院

收稿日期:2012-08-27 修回日期:2012-11-27 出版日期:2013-08-05 发布日期:2013-07-18
通讯作者: 徐红艳 E-mail:ywp_1983@126.com

The Hans，Choi immunophenotyping and c-myc genetic features between pediatric and adult diffuse large B-cell lymphoma

Received:2012-08-27 Revised:2012-11-27 Online:2013-08-05 Published:2013-07-18

摘要/Abstract

摘要： 目的探讨弥漫大B细胞淋巴瘤(DLBCL)各年龄组的Hans、Choi免疫分型及c-myc基因特征。方法收集60例DLBCL临床病理资料并进行随访，其中儿童组17例、成人组43例。用免疫组化SP法观察CD10、bcl-6、MUM1、FOXP1、GCET1及CD5蛋白表达，根据Hans及Choi免疫分型标准分型；用间期荧光原位杂交法检测c-myc基因。结果 1）Hans分型：儿童DLBCL组GCB型11例，non-GCB型6例；成人组DLBCL中GCB型9例，non-GCB型34例（P<0.05）。2）Choi分型：儿童DLBCL组GCB型13 例，ABC型4例；成人DLBCL组GCB型13例，ABC型30例（P<0.01）。3）c-myc基因：儿童组DLBCL中c-myc基因断裂6例；成人组DLBCL中c-myc基因正常43例（P<0.01）。结论儿童DLBCL以GCB型为主，预后较好；成人DLBCL以non-GCB或ABC型为主，预后相对较差，儿童DLBCL的c-myc基因断裂明显高于成人。

关键词: 儿童, 淋巴瘤, 免疫分型, 原位杂交

Abstract: Objective To explore the difference of Hans, Choi immunophenotyping and c-myc gene characteristics between pediatric and adult diffuse large B-cell lymphoma. Methods To Collect 60 cases of DLBCL clinicopathological data and follow-up, 17 cases of children group, adult group of 43 patients. To observe CD10, bcl-6, MUM1, FOXP1, GCET1 and CD5 protein expression by immunohistochemical SP method and accord to Hans and Choi immunophenotyping standard genotyping. To detect c-myc gene circumstances Interphase fluorescence insituhybridization (FISH) . Results 1) Hans typing：the Children DLBCL group of GCB were 11 cases, non-GCB type were 6 cases； the GCB type in the adult DLBCL group were nine cases, non-GCB type were 34 cases. 2) Choi typing：the Children DLBCL group of GCB type were 13 cases, ABC type were 4 cases； the adult DLBCL group of GCB type were 13 cases, ABC type were 30 cases (P = 0.008). 3) c-myc gene：The c-myc gene disruption of children DLBCL were 5 cases; c-myc gene in 43 adult cases were normal(P=0.006). Conclusions Child DLBCL are mainly GCB-type with better prognosis；but non-GCB-type are more common type in adult with worse prognosis.The c-myc gene disruption in Children DLBCL was significantly higher than that of adult ones.

Key words: Child, lymphoma, Immunophenotyping, Insituhybridization

徐红艳黄慧杨文萍黄传生钟梅慧. 儿童与成人弥漫大B细胞淋巴瘤的Hans、Choi免疫分型和c-myc基因特征[J]. 基础医学与临床, 2013, 33(8): 1038-1042.

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