基础医学与临床 ›› 2013, Vol. 33 ›› Issue (2): 189-193.

• 研究论文 • 上一篇    下一篇

人端粒酶反转录酶启动子调控的肿瘤特异性载体的构建及表达

侯睿1,金鑫2,李和2   

  1. 1. 华中科技大学同济医学院第二临床学院
    2. 华中科技大学同济医学院
  • 收稿日期:2012-04-16 修回日期:2012-07-12 出版日期:2013-02-05 发布日期:2013-01-25
  • 通讯作者: 李和 E-mail:heli@mails.tjmu.edu.cn
  • 基金资助:
    国家大学生创新基金

Establishment and expression of hTERT promoter modulated tumor specific expressing vector

  • Received:2012-04-16 Revised:2012-07-12 Online:2013-02-05 Published:2013-01-25

摘要: 目的: 构建由人端粒酶反转录酶(hTERT)启动子调控的具有肿瘤细胞特异性表达能力的荧光蛋白质粒。方法: 利用PCR技术克隆hTERT 基因启动子,利用分子生物学方法以该启动子替换pEGFP-C3质粒的CMV启动子,构建重组质粒(hTERTp-EGFP)。用脂质体转染法将hTERTp-EGFP质粒分别转染至肿瘤细胞和正常细胞, 观察此两种细胞内绿色荧光蛋白的表达差异。结果: 成功构建重组质粒hTERTp-EGFP,转染结果显示该质粒在肿瘤细胞中表达,而在正常细胞中无明显表达。结论: 重组质粒hTERTp-EGFP具有肿瘤特异性表达能力。

关键词: 端粒酶, 端粒酶逆转录酶启动子, 肿瘤, 质粒

Abstract: Objective: To construct a tumor specific expressing GFP plasmid modulated by hTERT promoter. Method: hTERT promoter was cloned by PCR, and used to substitute the CMV promoter of pEGFP-C3 plasmid by means of genetic engineering to form recombinant plasmid(hTERTp-EGFP), which was subsequently used to transfect tumor cell and non-tumor cell respectively. Results: The recombinant plasmid hTERTp-EGFP was successfully established, and its post-transfection expression was observed in tumor cells but not in non-tumor cells. Conclusion: Recombinant plasmid hTERTp-EGFP has the potential of tumor specific expression.

Key words: telomerase, hTERT-promoter, tumor, plasmid