基础医学与临床 ›› 2013, Vol. 33 ›› Issue (11): 1470-1474.

• 研究论文 • 上一篇    下一篇

神经调节蛋白-1β在压力超负荷大鼠肥大心肌组织中的表达下降

刘延峰1,刘小娟2,惠起源1   

  1. 1. 延安大学医学院
    2. 延安市卫生学校
  • 收稿日期:2013-01-11 修回日期:2013-03-26 出版日期:2013-11-05 发布日期:2013-10-28
  • 通讯作者: 惠起源 E-mail:qyhui@163.com

Decreased expression of Nneuregulin-1β in myocardium of hypertrophic rats induced by pressure-overloaded

  • Received:2013-01-11 Revised:2013-03-26 Online:2013-11-05 Published:2013-10-28

摘要: 目的 研究NRG-1β在压力超负荷心肌肥大大鼠心肌中的变化。方法 复制心肌肥大模型,将模型及假手术动物各随机分成2组:4周模型组(4wM)和周模型组(8wM组)。采用心动超声及血流动力学评价心功能;Masson染色观察心肌结构;放射免疫法检测心肌中血管紧张素II(AngII)含量;Real time-PCR及Westrrn blot检测心肌中神经调节蛋白-1(NRG-1β)mRNA及蛋白的表达。结果 1) 和4周对照组(4wC组)比较,4wM组左室射血分数(LVEF)、短轴缩短率(LVFS)、左室收缩末压(LVESP)、左室内压最大上升和下降速率(±dP/dtmax)下降(P <0.05),左室收缩末内径(LVEDD)、舒张末内径(LVESD)、左室舒张末压(LVEDP)及心肌胶原容积分数(CVF)增高(P <0.05,P <0.01),心肌中AngII表达增加(P <0.01)。2)和8周模型组(8wC组)比较,8wM组LVEF、LVFS、LVESP、±dP/dtmax下降(P <0.01), LVEDD、LVESD、LVEDP及CVF增高(P <0.05,P <0.01),心肌中AngII表达增加, NRG-1βmRNA及蛋白表达下降(P <0.01)。结论 NRG-1β在压力超负荷心肌肥大大鼠心肌中表达下调,可能参与了压力超负荷所致心肌肥大的发生和发展。

关键词: [关键词] NRG-1β, 压力超负荷, 心肌肥大

Abstract: Obective To investigate the change expression of neuregulin-1β(NRG-1β) at mRNA and protein levels in myocardium of hypertrophic rats induced by pressure-overloaded.Methods Wistar rats were randomly divided into four groups: myocardial hypertrophy groups (Model) of 4 weeks and 8 weeks, control operation groups (Control) of 4 weeks and 8 weeks. Model groups were operated with abdominal aortic constriction. Hemodynamic and echocardiogrophic characteristics were evaluated; masson staining was employed to observe the pathological lesions of myocardial tissues; radioimmunological method was used to detect angiotensin II (AngII); expression of NRG-1β mRNA and protein in myocardium were tested by Real time-PCR and western blot. Results (1) Compared with 4wC, left ventricular ejection fraction (LVEF)、left ventricular fraction shortening (LVFS)、left ventricular end systolic pressure (LVESP)、Maximal rate of increase in left ventricular pressure (±dP/dtmax) of 4wM were decreased(P <0.05),while left ventricular end-systolic diameter (LVEDD)、left ventricular end-diastolic diameter (LVESD)、left ventricular end diastolic pressure (LVEDP) 、 collagen volume fraction (CVF) and AngII of 4wM were increased(P <0.05,P <0.01),NRG-1β mRNA and protein expression of 4wM group were not exchanged(P >0.05)。 (2) Compared with 8wC, LVEF、LVFS、LVESP、±dP/dtmax of 4wM were decreased(P <0.01), LVEDD、LVESD、LVEDP、CVF and AngII were increased(P <0.05,P <0.01),NRG-1β mRNA and protein expression of 8wM group were decreased(P <0.01)。Conclusion The expression of NRG-1β in myocardium of hypertrophic rats induced by pressure-overloaded are down-regulated ,which may be involved in the myocardial hypertrophy development.

Key words: Key word: NRG-1β, pressure-overload, myocardial hypertrophy

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