关键词: 【关键词】miR-26a, 白血病细胞, 慢病毒, 细胞增

Abstract: 【Abstract】0bjective Construct the recombinant lentivirus vector expressing has-miR-26a, and to observe the effect of overexpression of miR-26a on acute myeloid leukemia cell proliferation. Method The lentiviral vector of MicroRNA-26a (LV-miR-26a-GFP) was constructed and transfected into human acute myeloid leukemia cells．The cells were divided into non-transfected group, transfected group (transfected with LV-miR-26a-GFP) and negative control group( transfected with LV-GFP)．The expression level of miR-26a was measured by real-time PCR. The expression of target gene PTEN was tested by Western blot. The cell growth curves and the effects of the miR-26a on the ability of cell proliferation were respectively analyzed by XTT and soft agar colony formation assay．Result The expression vectors of miR-26a restructuring lentivirus have been successfully achieved. After transfected acute myeloid leukemia cell NB4, successfully achieved the over expression o f miR-26a. Compared with non-transfected group and negative control group, the ability of cell proliferation for transfected with LV-miR-26a-GFP group was significantly increased (P < 0.01). The miR-26a overexpressed can inhibit the expression of target gene PTEN in NB4 cells. Conclusion MicroRNA-26a can inhibit the expression o f target gene PTEN, enhance the ability of acute leukemia cell proliferation, and promote cell proliferation.

Key words: 【Key words】 miR-26a, Leukemia cell, Lentivirus, cell proliferation