基础医学与临床 ›› 2012, Vol. 32 ›› Issue (4): 437-441.

• 研究论文 • 上一篇    下一篇

FGF10及其受体FGFR2 Ⅲb在小鼠肾脏发育中的表达

李佳,郭敏   

  1. 辽宁医学院组织学与胚胎学教研室
  • 收稿日期:2012-01-11 修回日期:2012-02-23 出版日期:2012-04-05 发布日期:2012-03-21
  • 通讯作者: 郭敏 E-mail:mguo1118@yahoo.com.cn
  • 基金资助:
    辽宁省教育厅科研项目

Expression of FGF10 and FGFR2Ⅲb in development of mouse kidney

  • Received:2012-01-11 Revised:2012-02-23 Online:2012-04-05 Published:2012-03-21

摘要: 摘要:目的 观察FGF10及其受体FGFR2 Ⅲb在小鼠肾脏发生发育中的表达和分布,探讨它们在肾脏发生发育中的作用。方法 选取胚龄12、14、16、18 d和生后1、7、14、21、42 d小鼠肾组织,用免疫组织化学和蛋白印迹技术(Western blot),检测FGF10和FGFR2 Ⅲb的表达。结果 E12 d FGF10及FGFR2Ⅲb开始在输尿管芽微弱表达;随着肾脏发育成熟,FGF10及FGFR2Ⅲb主要表达于远端小管和集合管;FGF10在近曲小管表达,近直小管无表达;FGFR2Ⅲb在近端小管无阳性表达;生后肾组织及发育各阶段肾小体未见FGF10及FGFR2Ⅲb表达。Western blot显示随着胚(日)龄的增加,FGF10及FGFR2Ⅲb在肾脏的表达量先增后减。结论 FGF10和FGFR2Ⅲb在肾脏发生发育过程中发挥重要作用。

关键词: FGF10, FGFR2Ⅲb, 肾发育, 小鼠

Abstract: Abstract:Objective To observe the expression and sites of FGF10 and FGFR2 Ⅲb during kidney development of mouse, in order to investigate their roles in kidney development. Methods Immunohistochemistry technique and Western blotting method were used to observe the expression of FGF10 and FGFR2 Ⅲb at embryonic day 12、14、16、18 and neonatal day 1、7、14、21、42 in the developing mouse kidney. Results FGF10 and FGFR2 IIIb were weakly expressed in the ureteric buds at E12d and then with the renal development, FGF10 and FGFR2 IIIb were expressed mainly in distal tubules and collecting ducts. FGF10 was expressed also in the proximal convoluted tubule. FGFR2 IIIb was not expressed in the proximal tubule. The renal corpuscles in each development stage were not found the expression of FGF10 and FGFR2 IIIb. Western blot showed that during kidney development, the expressions of FGF10 and FGFR2 IIIb were increased firstly and then decreased. Conclusions FGF10 and FGFR2 IIIb probably play an important role in the renal development.

Key words: FGF10, FGFR2Ⅲb, Kidney development, Mouse

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