蛇血清sPLA2抑制剂对人炎症的保护：基于结构与功能的研究

基础医学与临床 ›› 2011, Vol. 31 ›› Issue (9): 970-975.

蛇血清sPLA2抑制剂对人炎症的保护：基于结构与功能的研究

黄春洪

南昌大学医学院生物化学与分子生物学教研室

收稿日期:2010-08-23 修回日期:2010-09-28 出版日期:2011-09-05 发布日期:2011-09-05
通讯作者: 黄春洪 E-mail:merlynhuang@sohu.com
基金资助:
国家自然科学基金

Possible protective effects of secretory phospholipase A2 inhibitor from snake serum against human inflammation: based on protein structure and function

Received:2010-08-23 Revised:2010-09-28 Online:2011-09-05 Published:2011-09-05

摘要/Abstract

摘要： 摘要：目的比较人和蛇分泌型磷脂酶A2（secreted phospholipase A2，sPLA2）结构相似性，分析蛇血清磷脂酶A2抑制剂（phospholipase A2 inhibitor, PLI）对sPLA2特异性抑制的结构与功能关系，评价蛇血清PLI对人炎症的抑制作用。方法利用Clustal W对人和五步蛇sPLA2氨基酸序列进行多重序列比对。利用Swiss-model对各种sPLA2进行空间结构的同源建模预测，并使用Chiema软件对人和蛇毒sPLA2空间结构进行三维比对。结果人和五步蛇sPLA2一级序列同源性有40%左右，空间结构几乎相似。各种PLI具有相同的空间结构特征和保守的sPLA2结合区。结论根据蛋白质结构与功能关系，理论上推断蛇血PLI将可以有效抑制人sPLA2活性，对于sPLA2介导的炎症发生通路将有早期抑制效果和治疗意义。

关键词: 磷脂酶A2 , 磷脂酶A2抑制剂 , 生物信息学

Abstract: Abstract: Objective To compare the structural similarities of human secretory phospholipase A2 (sPLA2) with snake venom sPLA2, and to analyze the neutralization effect of sPLA2 inhibitors (PLIs) from snake serum against sPLA2 by exploring their structure and function relationship, which was meaningful for assessing PLIs’ clinical application in inflammation protection. Methods Multiple sequencial alignment of 7 human sPLA2 and 3 five-pace snake venom sPLA2 was made by Clustal W. Tertiary structures of the 10 sPLA2 were predicted by Swiss-Modelling. Spatial structure similarity between snake venom and human sPLA2 was assessed by UCSF Chiema. Results Human and snake venom sPLA2 shared 40% sequencial homologous and approximately similar spatial structure. All the PLIs (γ type) have similar tertiary charectristics and conserved binding region是 to sPLA2. Conclusion Based on the bioinformatical analysis, snake serum PLIs were considered as an effective inhibitor to snake venom toxicity and human sPLA2 activity. Thus, the PLIs were potential special blockers to human sPLA2 inflammation pathway and meaningful for curing clinical disease.

Key words: phospholipase A2, phospholipase A2 inhibitor, bioinformatics

中图分类号:

黄春洪. 蛇血清sPLA2抑制剂对人炎症的保护：基于结构与功能的研究 [J]. 基础医学与临床, 2011, 31(9): 970-975.

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