基础医学与临床 ›› 2011, Vol. 31 ›› Issue (5): 551-555.

• 研究论文 • 上一篇    下一篇

IL-17在慢性HBV感染及肝炎肝纤维化患者中表达升高

郑昭敏1,王延国2,张海涛1,杜文军1,陈士俊1   

  1. 1. 济南市传染病医院
    2. 莱芜矿业有限公司医院
  • 收稿日期:2010-04-12 修回日期:2010-08-23 出版日期:2011-05-05 发布日期:2011-05-06
  • 通讯作者: 陈士俊 E-mail:csj7516@sina.com
  • 基金资助:
    卫生科学研究基金

IL-17 expression in patients with chronic HBV infectious diseases and hepatic fibrosis

Zhao-min ZHENG1,Yan-guo WANG2,Hai-tao ZHANG2,Wen-jun DU2,Shi-jun CHEN1   

  1. 1. Jinan Infectious Disease Hospital
    2.
  • Received:2010-04-12 Revised:2010-08-23 Online:2011-05-05 Published:2011-05-06
  • Contact: Shi-jun CHEN E-mail:csj7516@sina.com

摘要: 目的 探讨IL-17与HBV感染特别是?肝纤维化发生的相关性。方法 酶联免疫吸附试验(ELISA)及反转录-多聚酶链反应(RT-PCR)法测定血清中IL-17浓度和外周血单个核细胞(peripheral blood mononuclear cell ,PBMC)中IL-17mRNA的水平。免疫组化法及ELISA法测定肝组织中IL-17的表达和IV型胶原、层粘连蛋白(laminin ,LN)、透明质酸(hyaluronidase,HA)及III型胶原的水平。结果 4组患者中血清IL-17和PBMC中IL-17mRNA水平表达均高于正常对照组(P均<0.005);与其他各组相比,IL-17及其mRNA水平在肝硬化患者中升高尤其明显(P均<0.005)。肝组织IL-17表达强度及血清肝纤维化指标水平在肝硬化组高于慢性乙肝组,慢性乙肝组高于携带者组(IL-17表达强度P均<0.01,肝纤维化指标P均<0.001);肝组织IL-17染色位于汇管区,与纤维化高度相关;淋巴细胞、成纤维细胞及血窦内皮细胞染色阳性。肝组织IL-17表达强度与血清Ⅳ型胶原,LN,HA,Ⅲ型胶原呈正相关(r=0.883,0.834,0.793,0.722,P均<0.01)结论 IL-17可能参与慢性HBV感染尤其是肝纤维化的发生过程。

关键词: 白细胞介素17, 乙型肝炎, 慢性, 肝纤维化, 肿瘤

Abstract: Objective To explore the role of interleukin-17(IL-17) in the pathogenesis and progress of chronic hepatitis B virus(HBV) infectious diseases, especially in liver fibrosis. Method Serum IL-17 level and liver fibrosis index type IVcollagen, LN,HA,type IIIcollagen was determined by enzyme linked immunosorbent assay(ELISA). IL-17 mRNA expression in peripheral blood mononuclear cell(PBMC) was analysed by reverse transcriptase-polymerase chain reaction(RT-PCR).IL-17 expression in liver was measured by immunohistochemistry. Result Serum IL-17 protein levels and IL-17 mRNA expression of PBMC in four HBV infectious groups (P<0.01each) were higher than in normal control. IL-17 and IL-17 mRNA in LC was higher compared with other groups(p<0.001 each). Liver IL-17 expression and serum fibrosis index raised as severity of disease with LC higher than CHB,CHB higher than ASC(Liver IL-17 expression p<0. 01 each, serum fibrosis index p<0. 001 each). IL-17 mainly located in portal area and was associated with fibrosis extent closely. Lymphocyte, fibroblast and sinusoid endotheliocyte were stained positively. Liver IL-17 expression was correlated with serum fibrosis index type IVcollagen, LN,HA,type IIIcollagen positively(r=0.883,0.834,0.793,0.722,P <0.01each) Conclusion IL-17 may contribute to the development of HBV infectious disease especially to the pathophysiology of LC.

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