Abstract: Objective To investigated whether Angiopoietin-1 (Ang-1) has direct cytoprotective effects on rat cardiomyocytes against oxidative stress and its possible signaling pathway involved.Methods A model for primary culture of cardiomyocytes injuried by H2O2 was established, the cultured cardiomyocytes were divided randomly into four groups.The morphology of cardiomyocytes was observed by Hoechst-33342, Apoptosis rate of cardiomyocytes was determined by flow cytometry (FCM), the expression of p-akt、active-caspase 3 were measured by western blot analysis.Results The flow cytometry(FCM) analysis showed that the percentage of apoptosis cells in control group and Ang-1 intervention group were 2.13±0.61%、31.20±2.01%，and compared with the induced group（48.16±1.37%）was significantly lower(P<0.01), and apoptosis rate of cointervention group was 47.42±2.02%,has no significant difference with induced group(p＞0.05). Western blotting analysis showed Ang-1 restored the phosphorylation of Akt and inhibited the activation of caspase 3,and these two effects were reversed by the presence of LY294002.Conclusion This study suggest that Angiopoietin-1 (Ang-1) has direct cytoprotective effects on cardiomyocytes against oxidative stress and this effects is dependent on the PI3K/Akt pathway and inhibition of caspase 3 cleavage.