用比较基因组杂交技术（CGH）检测肾癌染色体畸变

基础医学与临床 ›› 2010, Vol. 30 ›› Issue (4): 398-400.

用比较基因组杂交技术（CGH）检测肾癌染色体畸变

王坚徐仁方宋青峰

解放军第303医院常州市第一人民医院

收稿日期:2009-06-29 修回日期:2009-10-27 出版日期:2010-04-05 发布日期:2010-04-05
通讯作者: 徐仁方

Comparative genomic hybridization technique detectes the chromosomal aberration in renal carcinoma

Jian WANG, Ren-fang XU, Qing-feng SONG

303th Hospital of People's Liberation Army the First People's Hospital of Changzhou

Received:2009-06-29 Revised:2009-10-27 Online:2010-04-05 Published:2010-04-05
Contact: Ren-fang XU,

摘要/Abstract

摘要： 目的应用比较基因组杂交技术（CGH）分析原发性肾癌肿瘤组织中染色体异常变化，探讨肾癌细胞遗传物质的改变，揭示肾癌发生发展的内在本质及其与临床特征之间的关系。方法采用CGH技术对12例肾癌组织提取的全基因组DNA进行检测，以了解肾癌全基因组的变化。结果 CGH技术检测的12例肾癌标本中均有染色体的畸变（扩增和/或缺失），常见的扩增区是（1p、4p、5q、7p、9p、16p），常见的缺失区是（3q、4q、6q、9q、14q、18q）。结论原发性肾癌存在广泛的遗传物质不平衡现象，肾癌细胞染色体扩增和/或缺失可能是肾癌发生发展的基础。

关键词: 原发性肾癌比较基因组杂交染色体

Abstract: Objective To detecte the chromosomal aberration in the tissue cells of renal carcinoma and to evaluate the relationship between the chromosomal aberration and development of renal carcinoma. Methods CGH technology was used to analyze the global genomic aberration in the fresh cancer tissue cells of 12 patients with renal carcinoma .Results all of 12 cases deceted by CGH showed chromosomal aberrations.The common extension regions of renal carcinoma were 1p、4p、5q、7p、9p and 16p.The common deletion regions of renal carcinoma were 3q、4q、6q、9q、14q and 18q.Conclusion The hereditary material of renal carcinoma is unbalanced.The extension and/or deletion of chromosome are the base of the occurrence of renal carcinoma.

Key words: renal carcinoma, comparative genomic hybridization, chromosome

中图分类号:

R691.9

王坚徐仁方宋青峰. 用比较基因组杂交技术（CGH）检测肾癌染色体畸变[J]. 基础医学与临床, 2010, 30(4): 398-400.

Jian WANG; Ren-fang XU; Qing-feng SONG. Comparative genomic hybridization technique detectes the chromosomal aberration in renal carcinoma[J]. Basic & Clinical Medicine, 2010, 30(4): 398-400.