基础医学与临床 ›› 2010, Vol. 30 ›› Issue (3): 272-274.

• 研究论文 • 上一篇    下一篇

人肝癌P57kip2基因失表达的遗传不稳定性

易晓佳 申丽娟 章宗籍   

  1. 昆明医学院病理教研室 昆明医学院病理教研室
  • 收稿日期:2009-02-20 修回日期:2009-07-09 出版日期:2010-03-05 发布日期:2011-05-04
  • 通讯作者: 章宗籍

Genetic Instability of P57kip2 Misexpression in Human Hepatocellular Carcinoma

Xiao-jia YI, Li-juan SHEN, Zong-ji ZHANG   

  1. Dept. of Pathology ,Kunming Medical College Dept. of Pathology ,Kunming Medical College
  • Received:2009-02-20 Revised:2009-07-09 Online:2010-03-05 Published:2011-05-04
  • Contact: Zong-ji ZHANG

摘要: 目的 探讨肝细胞癌(HCC)P57kip2mRNA表达与遗传不稳定性之间的相互关系,以探索P57kip2基因失表达的机制。方法 用原位分子杂交技术检测肝癌组织中P57kip2mRNA表达,用PCR-聚丙烯酰胺凝胶电泳-银染法检测LOH和MSI。结果 在正常肝组织未见P57kip2mRNA表达,癌周肝硬化组与肝癌组阳性表达率均为26.7%(8/30)。3个位点均未发生LOH;在2个微卫星位点发生MSI, MSI总阳性率为16.7﹪。D11S1760位点发生MSI与P57kip2mRNA表达有关联性(p<0.05)。结论 P57kip2mRNA表达异常提示其可能在HCC发生过程中起重要作用。MSI可能是肝癌发生过程中P57kip2mRNA表达异常的原因之一。

关键词: 肝癌, P57kip2, 遗传不稳定性, 杂合性缺失, 微卫星不稳定性

Abstract: Objective To study the expressions of P57kip2 mRNA and genetic instability of P57kip2 in human hepatocellular carcinoma (HCC).Methods In situ hybridization(ISH) was used to detect the expression of P57kip2 mRNA in HCC,MSI and LOH were detected by using PCR-Polyacrylamide gel electrophoresis-silver staining method.Results There was no expression of P57kip2 mRNA in normal liver tissue. The expression rate of P57kip2 mRNA both in pericancerous cirrhosis and hepatocellular carcinoma was 26.67﹪(8/30). LOH was not identified in 30 cases on three microsatellite locies;there were 2 microsatellite locies showing MSI,the total rate of MSI was 16.7﹪. There was correlation between the MSI of D11S1760 locies and the expression of P57kip2 mRNA(p<0.05).Conclusion The disorder expression of P57kip2 mRNA indicated that P57kip2 may be involved in hepatocarcinogenesis and prognosis. MSI may be one of the reasons that lead to the disorder expression of P57kip2 mRNA in hepatocarcinogenesis and prognosis.

Key words: HCC, P57kip2, Genetic instability, LOH, MSI

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