关键词: 低氧预适应, 蛋白激酶C epsilon, 蛋白质组学, 蛋白表达量

Abstract: Objective Identify novel protein kinase C (nPKC )-interacted proteins in the cortex of hypoxic preconditioned mice. Methods The techniques of immunoprecipitation (IP) and two-dimensional electrophoresis (2-DE) combining with ImageMaster 2D Platinum software were applied to analyze the differential expressions of nPKC -interacted proteins; the target protein spots were identified by using matrix-associated laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and Western blot. Results Compared with control group, there were 34 upregulated protein spots and 20 downregulated protein spots in cytosolic fraction, while 27 upregulated prtein spots and 28 downregulated protein spots were determined in particulate fraction of cerebral cortex of HPC mice. The levels of nPKC -interacted HSP70 and 14-3-3 protein expressions increased significantly both in cytosolic and particulate fractions; but the protein level of nPKC -interacted HSP60 increased only in particulate fraction of cerebral cortex of HPC mice. Conclusion nPKC might be involved in the development of cerebral HPC via the regulation of its interacted proteins such as HSP60, HSP70 and 14-3-3 .

Key words: HPC, nPKC epsilon, Proteomics, Protein expression