沉默TROP2基因抑制胃癌BGC-823细胞迁移侵袭能力

基础医学与临床 ›› 2010, Vol. 30 ›› Issue (2): 165-169.

沉默TROP2基因抑制胃癌BGC-823细胞迁移侵袭能力

戴东方陈德玉邵世和

江苏大学临床医学院江苏大学附属附属医院

收稿日期:2009-05-25 修回日期:2009-07-03 出版日期:2010-02-05 发布日期:2010-02-05
通讯作者: 陈德玉

Slienced TROP2 gene to inhibit the migration and invasion of gastric cancer BGC-823 cells

Dong-fang DAI, De-yu CHEN, Shi-he SHAO

School of Clinical Medicine,Jiangsu University the Affiliated Hospital,Jiangsu University

Received:2009-05-25 Revised:2009-07-03 Online:2010-02-05 Published:2010-02-05
Contact: De-yu CHEN,

摘要/Abstract

摘要： 目的　探讨TROP2基因在胃癌细胞迁移侵袭过程中的作用。方法　用基因克隆技术构建针对TROP2的小干扰RNA（siRNA），瞬时转染胃癌BGC-823细胞株,荧光实时定量PCR和Western blot法检测细胞TROP2mRNA和蛋白表达水平, MTT法测定细胞增殖能力，Transwell小室实验观察细胞迁移侵袭能力。结果　酶切鉴定和DNA测序分析显示，TROP2靶向RNA干扰重组质粒构建成功。筛选得到最佳干扰效果质粒，该质粒转染细胞后，细胞增殖和迁移侵袭能力显著下降（P < 0. 05）。结论　干扰TROP2基因具有抑制胃癌BGC-823细胞迁移侵袭的作用，TROP2基因可能成为癌基因靶向治疗的分子靶点。

关键词: 胃癌, TROP2, RNA干扰, 迁移, 侵袭

Abstract: Objective To investigate the role of TROP2 in migration and invasion of human gastric cancer cells. Methods Small interfering RNA（siRNA）targeting TROP2 gene was constructed by gene clone technology and transfected into gastric cancer cell line BGC-823. The expressions of mRNA and protein were detected by Real-time quantitative PCR and Western blot assay after RNA interference. The proliferation capability was determined by MTT assay. Transwell assay was performed to assess the effect of TROP2 targeted RNA interference on the migratory and invasive properties of gastric cancer in vit ro. Results 　Enzyme digestion analysis and DNA sequencing showed that TROP2 targeted RNA interference recombinant plasmids were constructed successfully. The most effective recombinant plasmid was selected. After transfection , knockdown of TROP2 significantly impaired the proliferation ，migration and invasion capability of BGC-823 cells in vit ro（P < 0. 05）.Conclusion 　Interfering and down-regulating TROP2 gene can inhibit migration and invasion of gastric cancer cell line BGC-823，indicating that TROP2 gene is probably a target for gastric cancer gene therapy.

Key words: gastric carcer, TROP2, RNA interference, migration, invasion

中图分类号:

R735.2

戴东方陈德玉邵世和. 沉默TROP2基因抑制胃癌BGC-823细胞迁移侵袭能力[J]. 基础医学与临床, 2010, 30(2): 165-169.

Dong-fang DAI; De-yu CHEN; Shi-he SHAO. Slienced TROP2 gene to inhibit the migration and invasion of gastric cancer BGC-823 cells[J]. Basic & Clinical Medicine, 2010, 30(2): 165-169.

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