关键词: 寡脱氧核苷酸, 赖氨酸羟化酶2基因, Ⅰ型胶原

Abstract: Objective To discuss the relationship between the expression of PLOD2 and the formation of collagen I in the scar. Methods To detect the mRNA expression of PLOD2 and COLIα1 in the scar and normal skin tissue , as well as the content of COLIα1 by RT-PCR and western blot; design and synthesis three kinds of PLOD2 ASODN targeted against the translational initiation , the joint of the first and the second exons and termination codon respectively，transfected into NIH/3T3 mouse fibroblast cell and detect the mRNA expression of COLIα1 and formation of collagen I by RT-PCR and western blot .Results : the expression of PLOD2 in the scar tissue is significantly higher than that in the normal tissue（P<0.05）, three kinds of ASODNs exert inhibitory effect on the mRNA expression of COLIα1 and formation of collagen I in fibroblast（P<0.05）, during which the repressive efficiency of ASODN targeted against the translational initiation is the highest（P<0.001）; while the mRNA expression level of COLIα1 has no statistical difference in the scar tissue , normal tissue and the groups treated with three kinds of ASODN. Discussions the high expression of PLOD2 is tightly related to the formation of collagen I, PLOD2 ASODN can suppress the formation of collagen I post-transcriptionally.

Key words: oligodeoxyribonucleotide, procollage-lysine 2-oxoglutarate 5-dioxygenase 2, collagenⅠ