基础医学与临床 ›› 2010, Vol. 30 ›› Issue (2): 155-158.

• 研究论文 • 上一篇    下一篇

雷帕霉素对食管鳞癌细胞裸鼠移植瘤生长及mTOR/4EBP1信号通路的影响

侯桂琴 刘书漫 王莉莉 贾欣 王丽 薛乐勋   

  1. 郑州大学药学院
  • 收稿日期:2009-03-24 修回日期:2009-06-12 出版日期:2010-02-05 发布日期:2010-02-05

Effects of Rapamycin on growth and mTOR/4EBP1 Signalling Pathway in Xenograft of Esophageal Squamous Cell Carcinoma

Gui-qin HOU, Shu-man LIU, Li-li WANG, Xin JIA, Li WANG, Le-xun XUE   

  1. School of Pharmaceutical Science,Zhengzhou University
  • Received:2009-03-24 Revised:2009-06-12 Online:2010-02-05 Published:2010-02-05

摘要: 目的 体内观察雷帕霉素对食管鳞癌细胞生长及mTOR/4EBP1信号通路的影响。方法 给BALB/c-nude小鼠皮下注射食管鳞癌细胞株(EC9706),建立移植瘤模型,用雷帕霉素及顺铂对肿瘤进行治疗;用RT-PCR及Western blot等观察细胞形态及信号通路中各因子的变化。结果 与对照组相比,雷帕霉素对肿瘤生长具有显著抑制作用(P<0.05),联合顺铂处理组的抑瘤作用更明显;雷帕霉素在体内降低了mTOR和p-4EBP1的表达,并使4EBP1的表达水平升高。结论 雷帕霉素在体内能明显抑制食管鳞癌细胞EC9706裸鼠移植瘤的生长并抑制mTOR/4EBP1信号通路的活性。

关键词: 食管鳞癌, mTOR, 4EBP1, 雷帕霉素

Abstract: Objective The purpose of this study was to investigate the effects of rapamycin on tumor growth and mTOR/4EBP1 signaling pathway in xenograft of esophageal squamous cell carcinoma (ESCC) EC9706 cells. The expressions of factors in mTOR/4EBP1 signaling pathway of the tumor tissues mentioned above were analyzed by RT-PCR and Western blotting, respectively. Results Both rapamycin and cisplatin significantly inhibited the growth of transplantable tumors compared to control group (P<0.05) and their combination had a stronger effect than that of them alone (P<0.01). The results of RT-PCR and Western blotting revealed that rapamycin significantly decreased the expression of mTOR and p-4EBP1 but increased the expression of 4EBP1. Conclusion Rapamycin inhibits the mTOR/4EBP1 signalling pathway, resulting in the growth inhibition of xenograft of EC9706 cells.

Key words: esophegeal squamous cell carcinoma, mTOR, 4EBP1, rapamycin