褪黑素后干预促进急性坏死性胰腺炎大鼠硫氧还蛋白-1的表达

基础医学与临床 ›› 2010, Vol. 30 ›› Issue (10): 1059-1065.

褪黑素后干预促进急性坏死性胰腺炎大鼠硫氧还蛋白-1的表达

钟卫一唐国都梁志海

广西壮族自治区民族医院广西医科大学第一附属医院消化内科广西医科大学第一附属医院

收稿日期:2010-01-25 修回日期:2010-05-25 出版日期:2010-10-05 发布日期:2010-10-05
通讯作者: 唐国都

Melatonin post-intervention could promote the expression of thioredoxin-1 in rats pancreatic tissues with acute necrotizing pancreatitis

Wei-yi ZHONG, Guo-du TANG, Zhi-hai LINAG

Guangxi Minzu Hospital Dept. Gastroenterology, the First Affiliated Hospital of Guangxi Medical University

Received:2010-01-25 Revised:2010-05-25 Online:2010-10-05 Published:2010-10-05
Contact: Guo-du TANG,

摘要/Abstract

摘要： 目的观察褪黑素后干预对急性坏死性胰腺炎(ANP)大鼠表达硫氧还蛋白-1（TRX-1）的影响。方法大鼠随机分成对照组，ANP模型组（模型组），褪黑素后干预组（后干预组），每组各24只。模型组腹腔注射6％左旋精氨酸 25mL/kg 体重，间隔1h，共3次，诱发ANP；后干预组在诱发ANP后30min腹腔注射0.25%褪黑素20mL/kg 体重1次；对照组腹腔注射等容积生理盐水。术后6 、12 和24h分别处死8只大鼠，留取动脉血和胰腺组织标本待测。取胰腺组织做病理学检查并评分；测定胰腺组织匀浆丙二醛（MDA）、髓过氧化酶（MPO）的含量；ELISA检测血清TRX-1水平；免疫组织化学测定胰腺组织TRX-1的表达；RT-实时荧光定量PCR测定胰腺组织TRX-1 mRNA的表达。结果模型组胰腺组织病理评分及MDA、MPO水平较对照组显著升高(p<0.05)，后干预组显著回降(p<0.05)；模型组血清的TRX-1量在6和12 h较对照组显著降低(p<0.05)，后干预组较模型组显著增高(p<0.05)；后干预组胰腺组织TRX-1蛋白 and TRX-1mRNA表达峰值从模型组的制模后12 h 提前到制模后6 h。结论褪黑素后干预ANP大鼠可能是通过提高组织和血清TRX-1的水平，减轻大鼠胰腺组织的损伤。

关键词: 急性坏死性胰腺炎, 褪黑素, 硫氧还蛋白-1

Abstract: Objective To investigate the effect of melatonin post-intervention on the expression of thioredoxin-1(TRX-1) in rats with acute necrotizing pancreatitis(ANP). Methods Male Spraque-Dawley rats were randomly allocated to control group, ANP group，melatonin post-intervention group with 24 rats in each group .The rats in ANP group received three intraperitoneal injections of 25 ml/kg body weight 6%L-arginine at an interval of 1 h to induce ANP. The rats in melatonin post-intervention group received intraperitoneal injections of 20ml/kg body weight 0.25% melatonin after ANP induction. Rats in control group received intraperitoneal injections of same amount of saline. Rats were sacrificed at 6, 12 and 24 h after ANP induction. The pathological evaluation of pancreatic tissues was performed. The concentrations of malondialdehyde and myeloperoxidase in pancreatic tissues were measured. The serum level of TRX-1 was measured by ELISA. The expressions of thioredoxin-1 and thioredoxin-1mRNA in pancreatic tissues were confirmed by immunohistochemistry and RT-PCR, respectively.Results Comparerd to the control group, pathological scores, malondialdehyde ,and myeloperoxidase of pancreatic tissues in ANP group were significantly increased(p < 0.05),while they in melatonin post-intervention group were significantly lower (p < 0.05) than those in ANP group. Comparerd to the control group,serum TRX-1 contents in ANP group significantly decreased(p < 0.05) at 6- and 12 h points, while they in melatonin post-intervention group were significantly higher (p < 0.05) than those in ANP group. the peak value of TRX-1 protein and TRX-1mRNA expressions in pancreatic tissues shifted from 12 h after ANP induction in ANP group to 6 h after ANP group in melatonin post-intervention group.Conclusions Melatonin post-intervention reduced the injury of pancreatic tissue may be through increased tissue and serum TRX-1 levels in ANP rats.

Key words: Acute necrotizing pancreatitis, melatonin, thioredoxin-1

钟卫一唐国都梁志海. 褪黑素后干预促进急性坏死性胰腺炎大鼠硫氧还蛋白-1的表达[J]. 基础医学与临床, 2010, 30(10): 1059-1065.

Wei-yi ZHONG; Guo-du TANG; Zhi-hai LINAG. Melatonin post-intervention could promote the expression of thioredoxin-1 in rats pancreatic tissues with acute necrotizing pancreatitis[J]. Basic & Clinical Medicine, 2010, 30(10): 1059-1065.