刺五加多糖通过ERK信号转导途径诱导H446细胞 G2/M 期阻滞

基础医学与临床 ›› 2010, Vol. 30 ›› Issue (1): 59-62.

刺五加多糖通过ERK信号转导途径诱导H446细胞 G2/M 期阻滞

赵俊霞闫永鑫王彦玲韩硕闫蕴力

河北医科大学基础医学院细胞生物学教研室河北医科大学基础医学院细胞生物学教研室河北医科大学基础医学院细胞生物学教研室

收稿日期:2009-02-20 修回日期:2009-05-19 出版日期:2010-01-05 发布日期:2010-01-05
通讯作者: 闫蕴力

ASPS induces G2/M arrest of H446 cells by activation of ERK signal pathway

Jun-xia ZHAO, Yong-xin YAN, Yan-ling WANG, Shuo HAN, Yun-li YAN

Department of Cell Biology, Basic Medical College, Hebei Medical University Department of Cell Biology, Basic Medical College, Hebei Medical University

Received:2009-02-20 Revised:2009-05-19 Online:2010-01-05 Published:2010-01-05
Contact: Yun-li YAN

摘要/Abstract

摘要： 目的研究刺五加多糖（ASPS）对人小细胞肺癌H446细胞G2/M 期阻滞的诱导作用及对ERK信号传导途径的影响。方法流式细胞技术（FCM）检测H446细胞周期；Western blot分析检测ERK、p-ERK蛋白的影响。结果与对照组相比，各ASPS处理组G2/M期细胞所占的比例明显增高（P<0.01），G0/G1期细胞所占的比例没有差异；加入ERK抑制剂PD98059后， G2/M期和G0/G1期细胞所占的比例与对照组没有差异；p-ERK的量显著高于对照组（P<0.01），而对ERK蛋白表达没有明显影响。结论 ASPS可能通过激活ERK信号转导途径，诱导H446细胞发生G2/M期阻滞。

关键词: 刺五加多糖, 小细胞肺癌H446细胞株, G2/M期阻滞, ERK, P38

Abstract: Objective To investigate ASPS induced G2/M arrest in small cell lung cancer cell lines H446 and its effect on ERK MAP kinase signal transduction pathways. Methods Cell cycle phases were inspected by flow cytometery (FCM) ; Western blot analysis inspected the proteins of ERK, p-ERK; Results Compared with control group, G2/M phase cells increased with concentration increase significantly, G0/G1 phase cells were no difference, G2/M phase cells and G0/G1 phase cells were no difference when pre-incubated with PD98059 prior to exposure to ASPS of different concentrations, proteins of p-ERK were increased significantly, expression of ERK were no difference. Conclusion ASPS may induce G2/M arrest of H446 cells possibly by activation ERK MAP kinase pathways.

Key words: Acanthopanax senticosus polysaccharide, small cell lung cancer cell lines (H446), G2/M arrest, ERK, P38

中图分类号:

赵俊霞闫永鑫王彦玲韩硕闫蕴力. 刺五加多糖通过ERK信号转导途径诱导H446细胞 G2/M 期阻滞[J]. 基础医学与临床, 2010, 30(1): 59-62.

Jun-xia ZHAO; Yong-xin YAN; Yan-ling WANG; Shuo HAN; Yun-li YAN. ASPS induces G2/M arrest of H446 cells by activation of ERK signal pathway[J]. Basic & Clinical Medicine, 2010, 30(1): 59-62.

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