免疫程序对抗猪血浆纤维连接蛋白抗血清效价的影响

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (9): 970-973.

免疫程序对抗猪血浆纤维连接蛋白抗血清效价的影响

陆艳琦郭华沈键吴学军王章存周晓峰章静波

郑州铁路职业技术学院郑州铁路职业技术学院郑州铁路职业技术学院郑州市通源生物技术有限公司郑州轻工业学院中国医学科学院基础医学研究所北京协和医学院基础学院

收稿日期:2008-09-04 修回日期:2009-01-14 出版日期:2009-09-20 发布日期:2009-09-20
通讯作者: 陆艳琦

Influence of immunization procedure on antiserum titer of porcine plasma fibronectin

Yan-qi LU, Hua GUO, Jian SHEN, Xue-jun WU, Zhang-cun WANG, Xiao-feng ZHOU, Jing-bo ZHANG

Zhengzhou Railway Vocational﹠Technical College Zhengzhou Railway Vocational﹠Technical College Zhengzhou Tongyuan Biotechnology Limited Company Zhengzhou University of Light Industry Institute of Basic Medical Sciences, CAMS & PUMC

Received:2008-09-04 Revised:2009-01-14 Online:2009-09-20 Published:2009-09-20
Contact: Yan-qi LU,

摘要/Abstract

摘要： 目的制备兔抗猪FN抗血清，并探讨不同免疫途径、免疫次数及兔品种对抗血清效价水平的影响。方法用猪血浆纤维连接蛋白（FN）分别免疫纯种新西兰兔和杂种兔，将兔分别随机分成4组，即皮下注射1次及另加淋巴结注射5次组和3次组、肌肉注射接种1次及另加静脉注射5次组和3次组，用免疫双扩散法测定FN抗血清效价。结果两种免疫途径都可产生特异性抗血清。皮下+淋巴结途径抗血清效价明显高于肌肉+静脉注射（p<0.05），5次接种血清效价较3次高（p<0.05），新西兰兔血清效价高于杂种兔（p<0.05）。结论制备兔抗猪血浆FN血清，皮下+淋巴结注射和多次加强免疫是较好的免疫方法，纯种新西兰兔较杂种兔更易产生兔抗猪FN抗血清。

关键词: FN, 抗血清, 制备, 免疫途径

Abstract: Objective To prepare Rabbit anti-pig plasma Fibronectin (FN) serum and to explore the effect of different immune approaches, the number of immunization and species of rabbits on the antibody titer. Methods Inbred New Zealand rabbits and cross bred rabbits respectively were inoculated by different programs which were hypodermic injection of 1 plus lymph nodethen injection of 5 or 3 times , intramuscular injection of 1 plus intravenous injection of 5 or 3 times with porcine plasma FN. Then, FN antibody was determined by the method of double immundiffusion. Results New Zealand rabbits and cross bred rabbits could bring anti-FN by hypodermic injection plus lymph nodethen injection or intramuscular injection plus intravenous injection. In addition, the antibody titer of the former was obviously higher than that of the latter and 5 times intensive immunization was higher than 3 times intensive immunization. Conclusions To obtain Rabbit anti-pig plasma FN serum, it may be better to inoculate rabbits through hypodermic injection plus lymph nodethen injection and multiple immunization．Moreover, inbred New Zealand rabbits were easy to induce anti-FN than cross bred rabbits .

Key words: FN, Antiserum, Preparation, Route of immunization

中图分类号:

R392.7

陆艳琦郭华沈键吴学军王章存周晓峰章静波. 免疫程序对抗猪血浆纤维连接蛋白抗血清效价的影响[J]. 基础医学与临床, 2009, 29(9): 970-973.

Yan-qi LU; Hua GUO; Jian SHEN; Xue-jun WU; Zhang-cun WANG; Xiao-feng ZHOU; Jing-bo ZHANG. Influence of immunization procedure on antiserum titer of porcine plasma fibronectin[J]. Basic & Clinical Medicine, 2009, 29(9): 970-973.

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