8－硝基白杨素抑制HL-60细胞系增殖及促凋亡

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (9): 929-932.

8－硝基白杨素抑制HL-60细胞系增殖及促凋亡

岳军伍石华

邵阳医学高等专科学校

收稿日期:2008-03-14 修回日期:2008-12-09 出版日期:2009-09-20 发布日期:2009-09-20
通讯作者: 岳军

Effect of 8-nitrochyrsin on the proliferation and apoptosis of HL-60 cells in vitro

Jun YUE, Shi-hua WU

Shaoyang Medical College

Received:2008-03-14 Revised:2008-12-09 Online:2009-09-20 Published:2009-09-20
Contact: Jun YUE,

摘要/Abstract

摘要： 目的观察8-硝基白杨素(NOChR)抑制人急性髓性白血病细胞株 HL-60 细胞增殖和诱导凋亡作用。方法用MTT法检测HL-60细胞增殖活性；胎盼蓝染色细胞计数法测定细胞存活率，绘制细胞生长曲线；DNA凝胶电泳观察HL-60细胞凋亡；流式细胞术检测HL-60细胞凋亡率。结果 NOChR对HL-60细胞增殖具有抑制作用,呈浓度依赖性；NOChR显著抑制HL-60细胞生长；NOChR能有效地诱导HL-60细胞凋亡，呈浓度依赖性。结论 NOChR具有抑制HL-60细胞增殖和诱导细胞凋亡作用。

关键词: 人急性髓性白血病, 白杨素, 8－硝基白杨素, 细胞凋亡

Abstract: Objective To investigate the effect of 8-nitrochyrsin on cell proliferation and cell apoptosis of HL-60 cells in vitro. Methods 8-nitrochyrsin on proliferation of HL-60 cells was detected by MTT assay; The trypan blue exclusion method was used to count the number of viable and dead cells, then the survival rate and growth curve were achieved; DNA ladder bands were observed by DNA agarose gel electrophoresis; HL-60 cell apoptotic percentage was detected by flow cytometry with PI staining. Results 8-nitrochrysin inhibited proliferation of HL-60 cells in a concentration- dependent manner; 8-nitro- chyrsin posses the inhibitory effect of the proliferation and growth of HL-60 cells; 8-nitrochrysin posses the significantly effect of induce apoptosis of HL-60 cells. Conclusion 8-nitrochyrsin posses the inhibitory effect of the proliferation and growth of HL-60 cells And significantly induce apoptosis of HL-60 cells.

Key words: Human Acute myeloid leukaemia, Chrysin, 8-nitrochyrsin, Apoptosis

中图分类号:

岳军伍石华. 8－硝基白杨素抑制HL-60细胞系增殖及促凋亡[J]. 基础医学与临床, 2009, 29(9): 929-932.

Jun YUE; Shi-hua WU. Effect of 8-nitrochyrsin on the proliferation and apoptosis of HL-60 cells in vitro[J]. Basic & Clinical Medicine, 2009, 29(9): 929-932.

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