雄激素改善小鼠实验性自身免疫性卵巢早衰

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (7): 749-752.

雄激素改善小鼠实验性自身免疫性卵巢早衰

施晓波李娜林秋华廖灿范立青

中南大学湘雅二医院

收稿日期:2009-02-11 修回日期:2009-05-21 出版日期:2009-07-20 发布日期:2009-07-20

Effects of Androgen on Mouse Model of Autoimmune Ovary Failure

Xiao-bo SHI, Na LI, Qiu-hua LIN, Can LIAO, Li-qing FAN

the Second Xiangya Hospital, Central South University

Received:2009-02-11 Revised:2009-05-21 Online:2009-07-20 Published:2009-07-20

摘要/Abstract

摘要： 目的探讨雄激素对自身免疫性卵巢早衰的影响。方法 50只小鼠随机分为空白组、早晚治疗组及对应模型组，治疗及模型组注射小鼠透明带多肽建立卵巢早衰模型。早晚治疗组分别于免疫第15、20天起腹腔注射丙酸睾酮共15d。以阴道脱落细胞涂片、血抗透明带抗体浓度、卵巢中淋巴细胞浸润及成熟卵泡比例作为观察指标。结果对于性周期改变、血抗透明带抗体浓度、卵巢淋巴细胞浸润程度，模型组显著高于空白组，早治疗组显著低于模型组；血抗透明带抗体浓度早治疗组显著低于晚治疗组。对于卵巢成熟卵泡比例，模型组显著低于空白组，早治疗组显著高于模型组及晚治疗组。结论雄激素能显著改善小鼠自身免疫性卵巢早衰，早期治疗效果明显优于晚期。

关键词: 雄激素, 卵巢早衰, 自身免疫, 治疗

Abstract: Objective:To identify the effects of androgen on mouse model of autoimmune ovary failure. Methods: Fifty mice were chosen and randomly divided into five groups of ten, that were the control group, early therapeutic group and model group, late therapeutic group and model group. Autoimmune POF was induced in forty mouse by Pzp3. Then, animals were randomly divided into four groups of ten; two of the groups were treated with androgen from the 15th and 20th day after immune. The control group was treated with distilled water. The therapeutic efficacy of each treatment was evaluated by observing and measuring the changes in sexual cycles of mice, the serum level of AzpAb, infiltration of cells positively expressing CD45 in the ovary as well as the percentage of growing follicles of the ovary. Results: The sexual cycles, serum level of AzpAb, infiltration of cells positively expressing CD45 in the ovary of each model group was higher than control group; early therapeutic group was lower than model group; and serum level of AzpAb in early therapeutic group was lower than late therapeutic group. The percentage of growing follicles in the ovary of each model group was lower than control group; early therapeutic group was higher than model group and late therapeutic group. Conclusion: The use of androgen in mice with autoimmune POF could notably ameliorate the pathogenetic conditions of the disorder, and the treatment during prophase was apparently more effective than during the advanced stage.

Key words: androgen, premature ovary failure, autoimmune, therapy

施晓波李娜林秋华廖灿范立青. 雄激素改善小鼠实验性自身免疫性卵巢早衰[J]. 基础医学与临床, 2009, 29(7): 749-752.

Xiao-bo SHI; Na LI; Qiu-hua LIN; Can LIAO; Li-qing FAN. Effects of Androgen on Mouse Model of Autoimmune Ovary Failure[J]. Basic & Clinical Medicine, 2009, 29(7): 749-752.

[1]	孙兆庆闫波. CRISPR/ Cas9基因编辑技术在心血管领域中的研究进展[J]. 基础医学与临床, 2019, 39(6): 890-894.
[2]	李林林吴聪. 长链非编码RNA 在自身免疫性疾病中的研究进展[J]. 基础医学与临床, 2019, 39(4): 573-576.
[3]	崔云英王芬童安莉张响华李玉秀. 纤维肌性发育不良致肾血管性高血压的临床特征及诊断治疗[J]. 基础医学与临床, 2019, 39(3): 381-384.
[4]	苏文杨宏宇. 环状RNA在肿瘤中的研究进展[J]. 基础医学与临床, 2019, 39(3): 409-413.
[5]	吴海芳孟祥玉许恒毅傅芬. II型子宫内膜癌相关基因突变及靶向治疗研究进展[J]. 基础医学与临床, 2019, 39(2): 277-280.
[6]	覃娜王艳华. 抑癌基因甲基化与骨肉瘤的关系[J]. 基础医学与临床, 2019, 39(2): 256-259.
[7]	彭娇唐旭毛王导新. 内质网应激与急性呼吸窘迫综合征的研究进展[J]. , 2019, 39(12): 1763-1766.
[8]	章盈婧于健春. 肿瘤相关巨噬细胞在胃癌发展与治疗中的研究进展[J]. , 2019, 39(11): 1644-1648.
[9]	丁纳卢海妹刘亚坤姜玉阚立新. 异位骨化的预防与治疗[J]. 基础医学与临床, 2018, 38(8): 1153-1157.
[10]	李北芳高霞张朦琦葛赛李忠武沈琳高静. 运用组学手段探索EBV相关性与非相关性胃癌的可能差异分子[J]. 基础医学与临床, 2018, 38(7): 933-937.
[11]	张笑吴昊马百涛常健博陈亦豪马文斌李永宁王任直魏俊吉. 血液系统疾病患者合并慢性硬膜下血肿的治疗[J]. 基础医学与临床, 2018, 38(7): 999-1002.
[12]	童晨曦宋银宏. 嵌合抗原受体基因修饰的T细胞免疫治疗骨肉瘤的研究进展[J]. 基础医学与临床, 2018, 38(7): 1012-1015.
[13]	李海丽邵驰浩周若宇张永进李翠罗瑛. 抑癌基因与癌基因的最新研究进展[J]. 基础医学与临床, 2018, 38(7): 1029-1033.
[14]	丁艳张静陶然任君旭. 顺铂治疗导致卵巢早衰的研究进展[J]. 基础医学与临床, 2018, 38(6): 845-848.
[15]	王勤宁李旎邵国丰. B型主动脉夹层治疗进展[J]. 基础医学与临床, 2018, 38(5): 717-721.