人骨形态发生蛋白3抑制骨肉瘤细胞系MG63和U2OS的体外生长

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (7): 731-736.

人骨形态发生蛋白3抑制骨肉瘤细胞系MG63和U2OS的体外生长

吴丽美何焕玲李星星陈英华卫佳左国伟苗静琨王嫣周兰

重庆医科大学生物医学工程系

收稿日期:2008-08-06 修回日期:2008-10-31 出版日期:2009-07-20 发布日期:2009-07-20

Human bone morphogenetic protein 3(hBMP3) inhibited the growth of human osteosarcoma cells MG63 and U2OS in vitro

Li-mei WU, Huan-ling HE, Xing-xing LI, Ying-hua CHEN, Jia WEI, Guo-wei ZUO, Jing-kun MIAO, Yan WANG, Lan ZHOU

Dept.Biomedical Engineering of Chongqing Medical University

Received:2008-08-06 Revised:2008-10-31 Online:2009-07-20 Published:2009-07-20

摘要/Abstract

摘要： 目的研究人骨形态发生蛋白3（hBMP3）对骨肉瘤细胞系MG63和U2OS的生物学作用。方法 hBMP3的重组腺病毒和表达hBMP3的条件培养液（rhBMP3-CM）干预MG63和U2OS，台盼蓝拒染法、TUNEL法、吖啶橙/溴乙啶荧光双染法、Transwell小室和碱性磷酸酶活性测定法分别检测细胞增殖、凋亡、迁移以及成骨分化能力的变化；空白组不做任何处理，实验对照组用AdGFP组和rGFP-CM处理。结果实验对照组和空白组间差别无显著性。与各自的对照组相比，在观察时间内，两种处理均致实验组细胞：（1）存活率降低；（2）凋亡率增加；（3）穿膜细胞数减少；（4）碱性磷酸酶活性增加。结论 hBMP3对骨肉瘤细胞系具有抑制作用，并促其向成骨方向分化。

关键词: 骨形态发生蛋白, 骨肉瘤, 细胞增殖, 细胞凋亡, 细胞迁移, 碱性磷酸酶活性

Abstract: Objective The biological effects of BMP3 on osteosarcoma were investigated by treating human osteosarcoma cell lines MG63, and U2OS with human BMP3 (hBMP3). Methods Osteosarcoma cells in experimental groups were respectively treated with AdBMP-3 and rhBMP3-CM, control groups with AdGFP and rGFP-CM, the blank with neither. The abilities of their proliferation, apoptosis, transmigration and differentiation were respectively detected by trypan blue exclusion test, terminal deoynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) and acridine orange-ethidium bromide fluorescent stain (AO/EB), transwell-room and alkaline phosphatase(ALP) activity reagent kit. Results 1) All the indexes detected were not significantly different between two control groups. 2) Compared with control groups, the cell survival rates showed a significant decrease in experimental groups. 3) The apoptosis indexes increased. 4)The number of trans-membrane cell decreased 5) The activity of alkaline phosphatase increased after treating with AdBMP3 and rhBMP-3 for 3 days in MG63, 5 days in U2OS. Conclusions hBMP3 could inhibit osteosarcoma cells and promote bone formation.

Key words: Bone morphogenetic protein, Osteosarcoma, Proliferation, Transmigration, Apoptosis, Alkaline phosphatase

吴丽美何焕玲李星星陈英华卫佳左国伟苗静琨王嫣周兰. 人骨形态发生蛋白3抑制骨肉瘤细胞系MG63和U2OS的体外生长[J]. 基础医学与临床, 2009, 29(7): 731-736.

Li-mei WU; Huan-ling HE; Xing-xing LI; Ying-hua CHEN; Jia WEI; Guo-wei ZUO; Jing-kun MIAO; Yan WANG; Lan ZHOU. Human bone morphogenetic protein 3(hBMP3) inhibited the growth of human osteosarcoma cells MG63 and U2OS in vitro[J]. Basic & Clinical Medicine, 2009, 29(7): 731-736.

[1]	齐力侯艳宋丽华. 转录因子YY1促进下咽癌细胞的迁移能力[J]. 基础医学与临床, 2019, 39(9): 1320-1324.
[2]	童静凯江霞. 托伐普坦对小鼠内髓集合管细胞系水通道蛋白及肾小管功能的影响[J]. 基础医学与临床, 2019, 39(8): 1163-1167.
[3]	晋瑞金迎迎李嫚雷喆. 过表达脾酪氨酸激酶降低氧化应激诱导的小鼠成骨细胞凋亡[J]. 基础医学与临床, 2019, 39(7): 1013-1018.
[4]	许佐明谭川路远姚天尉吴贤建汪建初浦涧. 蛋白激酶B通过调控低氧诱导因子-1表达干预人肝癌细胞系SMMC-7721增殖[J]. 基础医学与临床, 2019, 39(3): 360-364.
[5]	张娟冯燕和水祥. 过表达miR-5195-3p对结直肠癌细胞凋亡的影响[J]. 基础医学与临床, 2019, 39(3): 385-391.
[6]	唐静胡玲刘奉李小山赵梓亦李国利. 异氟醚抑制低氧/复氧诱导的肾小管上皮HK-2细胞系内质网应激凋亡[J]. 基础医学与临床, 2018, 38(9): 1258-1262.
[7]	杨蓉王美美杨杨许晋徐晓龑施青刘璘琛. 沉默FSTL1减少衣霉素诱导软骨细胞内质网应激及细胞凋亡[J]. 基础医学与临床, 2018, 38(9): 1309-1314.
[8]	侯丛丛马晓骊陈虹黄秉仁陈等. YAF2靶向cyclin D1表达促进肿瘤细胞增殖[J]. 基础医学与临床, 2018, 38(6): 764-770.
[9]	吕文秀吴文韬郑士梅谢林虎丁伯平. 姜黄素抑制顺铂诱导人肾小管上皮细胞系HK-2的凋亡[J]. 基础医学与临床, 2018, 38(2): 185-188.
[10]	吴庆程阔菊姚浩旗黄河杨天德. 芒果苷对原代大鼠心肌细胞缺血缺氧凋亡的影响[J]. 基础医学与临床, 2018, 38(2): 169-173.
[11]	蔡惠美曹文瑜黄玉钿傅建英马燕燕. 高迁移率族蛋白1促进原发性肝癌细胞增殖[J]. 基础医学与临床, 2018, 38(10): 1417-1421.
[12]	金涛彭昊王艳林吴红艳. 辣椒素诱导人骨肉瘤MG-63细胞发生免疫原性细胞死亡[J]. 基础医学与临床, 2017, 37(7): 918-922.
[13]	刘幸田甜詹玮余蕾韩冰谢汝佳罗新华杨勤. 辛二酰苯胺异羟肟酸对人肝星状细胞增殖和凋亡的影响[J]. 基础医学与临床, 2017, 37(4): 468-472.
[14]	李春艳童安莉王芬崔云英闫朝丽. 辛伐他汀对血管紧张素II刺激的肾上腺皮质癌H295R细胞的分泌和增殖的影响[J]. 基础医学与临床, 2017, 37(3): 346-350.
[15]	李波谭明贤胡海艳. KLF4过表达抑制人白血病K562细胞系的增殖及迁移[J]. 基础医学与临床, 2017, 37(11): 1574-1578.