基础医学与临床 ›› 2008, Vol. 28 ›› Issue (8): 841-845.

• 研究论文 • 上一篇    下一篇

高容量血液滤过减轻脂多糖诱导犬急性肺损伤

刘芬 曾振国 江榕 钱克俭 吕农华   

  1. 江西南昌大学第一附属医院ICU 江西南昌大学第一附属医院ICU 江西南昌大学第一附属医院ICU 江西南昌大学第一附属医院ICU 江西南昌大学第一附属医院消化科
  • 收稿日期:2007-09-06 修回日期:1900-01-01 出版日期:2008-08-25 发布日期:2008-08-25
  • 通讯作者: 吕农华

High volume hemofiltration relieve acute lung injury induced by LPS in dogs

Fen LIU, Zhen-guo ZENG, Rong JIANG, Ke-jian QIAN, Nong-hua LV   

  1. The First Hospital Affiliated of Nanchang University The First Hospital Affiliated of Nanchang University
  • Received:2007-09-06 Revised:1900-01-01 Online:2008-08-25 Published:2008-08-25
  • Contact: Nong-hua LV

摘要: 目的 研究高容量血液滤过(HVHF)对脂多糖(LPS)诱导犬急性肺损伤(ALI)的治疗作用,并探讨其机制。方法 健康犬16条,静脉注入LPS(650μg/kg)后,随机分为对照组(C组)和HVHF治疗组(T组)。监测动脉血气,放免法测定血TNF-α、IL-6和IL-10含量,流式细胞仪测定肺组织NF-κB活性,免疫印迹法测定肺表面活性蛋白(SP-B)含量,电镜下观察肺组织病理改变。结果 动物注入LPS后PaO2和PaO2/FiO2下降,HVHF治疗后上述指标明显高于C组(P<0.01)。治疗1h血中TNF-α、IL-6和IL-10的浓度即有明显下降,低于同时点C组 (P<0.01)。治疗4h后,NF-κB活性度下降 (P<0.01),而肺SP-B的含量明显高于C组(P<0.01)。电镜下T组肺间质及肺泡炎性水肿出血、中性粒细胞浸润、Ⅱ型肺泡上皮细胞损伤均较C组明显减轻。结论 HVHF减轻ALI可能与其清除炎症介质,抑制NF-κB活化,下调细胞因子高表达和炎症反应,减轻肺间质及肺泡炎性水肿,增加肺SP-B含量有关。

Abstract: Objective To research the mechanism of High Volume Hemofiltration (HVHF) on acute lung injury (ALI) induced by LPS in dogs . Methods After injected LPS (650μg/kg) via central vein within 30min, Sixteen healthy hybrid male dogs were divided into control group and treatment group randomly (n=8) .PH、PaO2 、PaCO2 in artery blood were recorded . Contents of TNF-α、IL-6 and IL-10 in plasm were measured by radioimmunity . The activity of NF-кB in lung homogenate was measured by flow cytometer . The content of surfactant protein B (SP-B) in lung homogenate was measured by protein Western-blotting.Changes of lung histopathology was observed via electron microscope. Results After injection of LPS , PaO2 and PaO2/FiO2 began to decrease. PaO2 and PaO2/FiO2 in treatment group kept higher than that in control group (P<0.01) . In treatment group, the content of TNF-α、IL-6、IL-10 in plasm obviously decreased after HVHF and the activation of NF-κB were also decreased. There was very significant difference at the same time between two groups (P<0.01). The content of SP-B in lung homogenate in treatment group was significantly higher than that in control group (P<0.01). Edema of alveoli and lung mesenchymal,hemorrhage,infiltration of inflammatory cells and the injury of PC-II in treatment group were obviously lightened after HVHF . Conclusion HVHF can relieve ALI induced by LPS in dogs, which is contributed to the continuously elimination of inflammatory medators , the inhibition of activation of NF-κB then the down regulation of high expression of cytokines , the attenuation of mesenchymal and alveolar inflammatory edema , and the increase of content of SP-B.