基础医学与临床 ›› 2008, Vol. 28 ›› Issue (8): 831-834.

• 研究论文 • 上一篇    下一篇

反义VEGF寡核苷酸联合放射线抑制食管癌细胞移植瘤生长

封巍 祝淑钗 王玉祥   

  1. 河北医科大学第四医院放疗科 河北医科大学第四医院放疗科 河北医科大学第四医院放疗科
  • 收稿日期:2007-09-04 修回日期:2007-12-25 出版日期:2008-08-25 发布日期:2008-08-25
  • 通讯作者: 祝淑钗

Irradiated combined with antisense VEGF therapy for esophageal cancer xenografts

Wei FENG, Shu-chai ZHU, Yu-xiang WANG   

  1. The Forth Hospital,Hebei Medical University The Forth Hospital,Hebei Medical University
  • Received:2007-09-04 Revised:2007-12-25 Online:2008-08-25 Published:2008-08-25
  • Contact: Shu-chai ZHU,

摘要: 目的 研究干扰VEGF表达联合照射对移植瘤的影响,为抗VEGF基因治疗联合放射治疗食管癌提供理论依据。方法 将16只裸鼠分为4组: ⑴对照组 ⑵ 转染组 ⑶照射组 ⑷转染加照射组。食管癌TE-1移植瘤直径0.8cm时开始实验,照射后反义VEGF 寡核苷酸转染。观察各组裸鼠移植瘤体积变化与肿瘤生长延迟时间,应用RT-PCR、western blot检测移植瘤组织中VEGF的表达。结果 转染组、照射组、转染加照射组的绝对延迟时间分别为(3.0±2.6)、(10.1±5.4)和(27.4±3.1)d,标准化的延迟时间为(24.4±3.1)d,对放射的增益因子为2.41。转染可降低VEGF 表达。结论 反义VEGF能显著提高食管癌细胞株TE-1裸鼠移植瘤的放射效应。

Abstract: Objective To investigate the effect of antisense VEGF on the radiation sensitivity of esophageal cancer cells in vivo.Methods 16 male BLBA/C/nu nude mice were randomly divided into 4 groups and prepared cells were subcutaneously injected on the paw pat of mice(2×10 6/100μl/mice): The volume of the subcutaneous tumors was measured before and after irradiated and transfected.The effect of treatment was assessed by tumor growth delay . The expression level of VEGF were examined in different groups by RT-PCR ,western blotting respectively..Results The absolute growth delay (AGD)for antisene group, irradiation group and antisene plus irradiation group was3.0±2.6, 10.1±5.4 and 27.4±3.1 days,and nominal growth delay (NGD) was 24.4±3.1 days . The enhancement factor of antisense VEGF for radiotherapy was 2.41.The decreased expression level of endogenous VEGFmRNA and protein were found in antisene group by RT-PCR and western blotting analysis.Conclusions Combining Antisense VEGF and radiotherapy could enhance radiotherapic effect for esophageal cancer xenografts