基础医学与临床 ›› 2008, Vol. 28 ›› Issue (8): 801-804.

• 研究论文 • 上一篇    下一篇

压力负荷性左室肥厚大鼠心肌钠钙交换体和肌浆网钙泵的表达

苏丹 成建华 戴亚蕾 吴大正 石海莲   

  1. 同济大学 医学院 药理学教研室 同济大学 医学院 药理学教研室 同济大学 医学院 免疫学教研室 上海中医药大学
  • 收稿日期:2007-07-28 修回日期:2007-10-29 出版日期:2008-08-25 发布日期:2008-08-25
  • 通讯作者: 戴亚蕾

Changes of NCX and SERCA2a gene expression in myocardial tissues in rats with pressure overload-induced left ventricular

Dan SU, Jian-hua CHENG, Ya-lei DAI, Da-zheng WU Hai-lian SHI   

  • Received:2007-07-28 Revised:2007-10-29 Online:2008-08-25 Published:2008-08-25
  • Contact: Ya-lei DAI,

摘要: 目的 观察压力负荷性左室肥厚大鼠心功能异常及心肌钠钙交换体(NCX)和肌浆网钙泵(SERCA2a)的表达变化。方法 缩窄大鼠腹主动脉制备压力负荷性心肌肥厚模型,测定在体血流动力学及左室重量指数(left ventricular weight index,LVWI),用RT-PCR和Western blot法检测左室组织NCX及SERCA2a基因的表达。结果 与假手术组相比,模型大鼠左室收缩压(LVSP)及左室舒张末压(LVEDP)均显著升高(P<0.01,P<0.001);左室重量指数显著增加(P<0.001)及左室NCXmRNA表达上调(P<0.05),蛋白表达无明显变化,SERCA2a基因表达下调(P<0.05)。结论 压力负荷性左室肥厚大鼠产生明显的心功能异常,尤以舒张功能障碍为著,其机制可能与SERCA2a基因表达下调有关。

关键词: 左室肥厚, 心功能, 钠钙交换体(NCX), 肌浆网钙泵(SERCA2a), 基因表达

Abstract: Objective To observe the change of NCX,SERCA2a gene expression in myocardial tissues and abnormity of heart function in rats with pressure overload-induced left ventricular hypertrophy. Methods cardiac hypertrophy was induced by clipping the abdominal aorta in rats. The parameters of hemodynamics including LVSP,LVEDP,±dp/dtmax,and HR were measured.The cardiac hypertrophy was evaluated by Left ventricular index(LVWI,left ventricular weight/body weight). NCX,SERCA2a mRNA and protein expressions in left ventricular tissues were determined by half-quantitative RT-PCR and Western blot normalized to abundance of GAPDH mRNA and protein,respectively. Result LVSP and LVEDP were obviously enhanced(P<0.01,P<0.001) in rats with cardiac hypertrophy in vivo. LVWI was increased remarkably(P<0.001)in model group. RT-PCR and Western blot results demonstrated that NCXmRNA expression was upregulated(P<0.05),and SERCA2a mRNA and protein expression were downregulated(P<0.05) significantly in model group compared with sham group. Conclusion Abnormity of heart function,especially diastolic function was observed. in rats with pressure overload-induced cardiac hypertrophy,and that may be relative to down-regulation of SERCA2a expressions in left ventricular tissues.

Key words: left ventricular hypertrophy, heart function, NCX, SERCA2a, gene expression.