氨基胍对大鼠纤维化肺成纤维细胞表达α-平滑肌肌动蛋白的影响

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (7): 770-771.

氨基胍对大鼠纤维化肺成纤维细胞表达α-平滑肌肌动蛋白的影响

孙玉敏张丽萍宋福成董静

沧州医学高等专科学校沧州医学高等专科学校天津武警医学院研究生队沧州医学高等专科学校

收稿日期:2007-11-09 修回日期:2007-12-10 出版日期:2008-07-25 发布日期:2008-07-25
通讯作者: 孙玉敏

Effects of AG to the expression of α-SMA on pulmonary fibroblasts from pulmonary fibrosis model rats

Yu-min SUN, Li-ping ZHANG, Fu-cheng SONG, Jing DONG

Received:2007-11-09 Revised:2007-12-10 Online:2008-07-25 Published:2008-07-25
Contact: Yu-min SUN,

摘要/Abstract

摘要： 摘要：目的探讨氨基胍（Aminoguanidine, AG）对转化生长因子-β1（Transforming growth factor -beta1, TGF-β1）诱导大鼠纤维化肺成纤维细胞表达 -平滑肌肌动蛋白（ -smooth muscle actin, α-SMA）的影响。方法用MTT法选择药物浓度。应用免疫细胞化学技术测定α-SMA阳性细胞百分率，用Motic图象分析系统测定其平均积光灰度值。应用流式细胞技术测定单个细胞表达α-SMA的均道值。结果 α-SMA阳性细胞百分率、平均积光灰度值及均道值AG单独刺激组与对照组比无明显差异，TGF-β1单独刺激组（10ng/ml）明显高于对照组（P＜0.01）；AG（500μM）+ TGF-β1（10ng/ml）联合刺激组低于TGF-β1单独刺激组（P＜0.05）。结论 AG单独作用不影响大鼠纤维化肺成纤维细胞表达α-SMA，但是AG能够削弱TGF-β1诱导大鼠纤维化肺成纤维细胞表达α-SMA的能力。

关键词: 氨基胍, 转化生长因子-β1, 肌成纤维细胞, 肺纤维化

Abstract: Abstract: Objective To observe the effects of aminoguanidine(AG) on transforming growth factor -beta1(TGF-β1) induced expression of -smooth muscle actin( -SMA) of the fibroblasts in lung from pulmonary fibrosis model rats in vitro. Methods The pulmonary fibroblasts from pulmonary fibrosis model rats were isolated from explants . We selected the proper concentrations of TGF-β1 and AG by MTT. The positive cells -SMA were observed by immunocytochemical staining. The expression of -SMA was examined by flow cytometry. Results The expression of -SMA of fibroblasts in TGF-β1 group were higher than those of Sham group ( P<0.01). Compared with TGF-β1 group, the expression of -SMA in AG+TGF-β1 group were lower (P<0.05); but compared with Sham group, they were higher significantly(P<0.01).There were no significant difference between AG group and Sham group（P>0.05）.Conclusion TGF-β1 induced the expression of -SMA in pulmonary fibroblasts from rats on d14 after administration of BLMA5 and accelerated the differentiation of fibroblasts to myofibroblasts. However, AG decreased these roles of TGF-β1.

Key words: aminoguanidine, transforming growth factor -beta 1, myofibroblast, pulmonary fibrosis

孙玉敏张丽萍宋福成董静. 氨基胍对大鼠纤维化肺成纤维细胞表达α-平滑肌肌动蛋白的影响[J]. 基础医学与临床, 2008, 28(7): 770-771.

Yu-min SUN; Li-ping ZHANG; Fu-cheng SONG; Jing DONG. Effects of AG to the expression of α-SMA on pulmonary fibroblasts from pulmonary fibrosis model rats[J]. Basic & Clinical Medicine, 2008, 28(7): 770-771.

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