关键词: 磁共振频谱, 肌肉, 谷氨酸, 谷氨酰胺, 定量, 新西兰兔

Abstract: Objective: Try to Use MRS technology to detect Glutamate & Glutamine’s total concentration in Zelanian rabbits’ in vivo muscle. Via biopsy, prove the accuracy of this method. Methods: Select 20 healthy Zelanian rabbits, using SS-PRESS sequence to collect their Signal Ratio of Glx/TCr. After the collection, let blood and do the biopsy of the observed area of the collected muscle tissue immediately. Mensurate the rabbits’ blood Crn concentration, Glx and TCr concentration in muscle. Using SPSS software to research the correlation between the Glx/TCr Signal Ratio and Glx/TCr concentration in muscle, attempt using the MRS Signal Ratio and blood Crn concentration to predict Glx concentration in muscle. Results: The correlation between Glx/TCr Peak Ratio and Glx/Crn ratio is 0.681. We performed a linear regression and got the formula: The predict Value of Glx concentration in muscle （umol/g muscle）= Glx/TCr Peak Ratio × Crn concentration in blood（mg/dl）× 28.754－0.631. Conclusion: Using the MRS method, the detected concentrations of the substances had a strong positive correlation with the biopsy values. Using a linear regression formula to predict the Glx concentration, the results can reflect the level of the true values, though it’s not accurate enough for quantitatively analysis, the new method can avoid biopsy.

Key words: MRS, Muscle, Glutamate, Glutamine, Quantitatively measurement, Zelanian Rabbit