Abstract: Objective To construct adenovirus vector with agiostatink1-5 gene and investigate the function of suppression to proliferation and migration for vascular endothelial cells.Methods With the use of gene recombination and clone technology ,we construct the adenovirus vector with the gene of angiostatin k1-5. Ad-angiostatin k1-5 was isolated from a single plaque,expanded in 293 cells and purified by cesium chloride contrifugation. In vitro vascular endothelial eclls proliferation assay and migration activity were investigated through direct infection,MTT and transwell chemotaxis assay. Results 50%TCID indicated that the condence of resultant viruses was 1.5×109PFU/mL. It was purified by CsCL banding,final yield were generally 1.1×1010 PFU/mL plaquing-forming units. Through indirect infect assay and MTT, we found angiostatin k1-5 could inhibit human vascular endothelial cells proliferation. We utilized human vascular endothelial cells to study the effect angiostatin k1-5 on cell migration ,the result showed that adenoviruse vector with angiostatin k1-5 could significantly inhibited HUVEC migration .Conclusions: We successfully constructed adenoviruse vector with angiostatin k1-5 and showed in vitro it could inhibit proliferation and migration of HUVEC.