基础医学与临床 ›› 2008, Vol. 28 ›› Issue (4): 335-339.

• 研究论文 • 上一篇    下一篇

大鼠脑皮层冲击伤中Bcl-2、Bax的表达变化

任东青 王琦 赵涛 张杰 曾桂英   

  1. 解放军第四军医大学军事防预医学系放射医学教研室 解放军第四军医大学西京医院急诊科 解放军第四军医大学军事防预医学系放射医学教研室 解放军第四军医大学 解放军第四军医大学军事防预医学系放射医学教研室
  • 收稿日期:2007-04-06 修回日期:2007-07-10 出版日期:2008-04-25 发布日期:2008-04-25
  • 通讯作者: 任东青

Changes of Bcl-2 and Bax expression in the cortex of blast injuried rat

Dong-qing REN, Qi WANG, Tao ZHAO, Jie ZHANG, Gui-ying ZENG   

  1. Faculty of Military Preventive Medicine, PLA of the Fourth Military Medical University
  • Received:2007-04-06 Revised:2007-07-10 Online:2008-04-25 Published:2008-04-25
  • Contact: Dong-qing REN,

摘要: 目的 探讨爆炸冲击伤中大鼠脑皮层Bcl-2、Bax基因表达的变化规律。方法 应用爆炸冲击波致伤大鼠,致伤后不同时间(12~96h),光镜和电镜观察脑皮层形态变化;免疫组织化学方法观察BCL-2和BAX蛋白在脑皮层表达;凝胶电泳法观察大鼠脑组织DNA电泳结果。结果 爆后12~96h,光镜和电镜观察脑皮层细胞凋亡改变增多;爆后24h脑组织Bcl-2、Bax基因表达阳性细胞率均最高,BCL-2/BAX表达比值随时间延长而降低;脑组织DNA电泳在爆后12、24和48h组形成典型阶梯状条带;上述改变以24h组最为显著。结论 爆炸冲击伤可导致部分脑皮层神经元的细胞凋亡,BCL-2和BAX表达比值下降。

Abstract: Objective To observe changes in the Bcl-2 and Bax gene expressions in rat brain mantle during the explosive blast injury. Methods Explosion shock was used to induce blast injury in the rat. From 12h to 96h after the blast injury, light microscope and electron microscope were used to observe the morphologic changes in rat brain mantle. Immunochemical method was used to study the changes in the expression of BCL-2 and BAX proteins in rat brain mantle, and gel electrophoresis was used to observe the changes in DNA ladder. Results During the postburst (12~96h), the increased apoptosis in brain mantle was observed by light microscope and electron microscope. The rate of BCL-2 and BAX positive cells has been the highest 24h after injury, with time their rate descending gradually in brain after the explosion. The DNA electrophoresis showed a typical ladder shape in 12h group, 24h group and 48h group. Above-mentioned changes were the most significant in 24h group. Conclusion The explosive blast injury could cause the apoptosis of some of the cerebral cortical neurons and the decline of BCL-2 /BAX rate.