ERK1和P16在人胃肠道间质瘤组织中的表达及相关性

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (12): 1292-1295.

ERK1和P16在人胃肠道间质瘤组织中的表达及相关性

俞薇薇瞿伟熊小亮刘繁荣袁晟

南昌大学医学院病理教研室南昌市第三医院南昌大学医学院病理学教研室南昌大学医学院病理学教研室江西省人民医院

收稿日期:2007-11-28 修回日期:2008-04-18 出版日期:2008-12-25 发布日期:2008-12-25
通讯作者: 俞薇薇

The Expression of ERK1 and P16 and Their Correlation in Gastrointestinal Stromal Tumor of Human

Wei-wei YU, Wei QU, Xiao-liang XIONG, Fan-rong LIU, Sheng YUAN

Department of Pathology,Medical College of Nanchang Unversity

Received:2007-11-28 Revised:2008-04-18 Online:2008-12-25 Published:2008-12-25
Contact: Wei-wei YU,

摘要/Abstract

摘要： 目的检测ERK1和P16 在胃肠道间质瘤组和正常对照组中的表达，探讨其在胃肠道间质瘤(GIST)发生和发展中的作用和相互关系。方法采用组织芯片技术和免疫组化Elivision二步法检测40例胃肠道间质瘤组和40例正常对照组中ERK1和P16的表达，应用图像分析软件Image-Pro Plus 6.0对ERK1和p16的表达行平均吸光度分析。结果与正常组织相比，胃肠道间质瘤组织中ERK1的表达明显增高（ P＜0.01），而P16的表达明显降低（P＜0.01）。ERK1和P16的表达与组织学分级密切相关（P＜0.05），而与年龄、肿块大小、组织学类型和复发无相关性。胃肠道间质瘤中ERK1与P16的表达呈负相关（P＜0.05）。结论在胃肠道间质瘤组织中，ERK1明显增高，P16则明显降低。两者之间存在显著负相关。

Abstract: Objective To detect the expression of ERK1 and P16 in gastrointestinal stromal tumor group and normal control group and to discuss the correlation between them. Methods Tissue chip and immunohistochemistry named Elivison were used to respectively detect the expression of ERK1 and P16 in both 40 cases of gastrointestinal stromal tumor and 40 cases of normal control tissues and quantitative analysis of mean absorbent density of the expression of ERK1 and P16 was conducted respectively with the image analytic software: Image-Pro Plus (version 6.0) . Results The expressions of ERK was higher in GIST group than that in normal control group(P＜0.01), but the expression of P16 was lower（P＜0.01）. The expression of ERK1 and p16 were correlated with histological grade (P＜0.05) and not correlated with age, lump, histological type and recurrence .There was an inverse correlation between the activity of ERK1 and P16 in GIST（P＜0.05）. Conclusion In the tissue of GIST the expression of ERK1 was remarkable high and P16 was low.There was an inverse correlation between them.

俞薇薇瞿伟熊小亮刘繁荣袁晟. ERK1和P16在人胃肠道间质瘤组织中的表达及相关性[J]. 基础医学与临床, 2008, 28(12): 1292-1295.

Wei-wei YU; Wei QU; Xiao-liang XIONG; Fan-rong LIU; Sheng YUAN. The Expression of ERK1 and P16 and Their Correlation in Gastrointestinal Stromal Tumor of Human[J]. Basic & Clinical Medicine, 2008, 28(12): 1292-1295.