关键词: 膀胱癌 EZH2 基因芯片 RNA干扰

Abstract: Objective To screen the gene expression profiles of human bladder cancer cell line EJ, in which RNA interference was used to downregulate the EZH2 expression , with gene chip and investigate the EZH2 molecular mechanism in cancer. Methods The vector expressing EZH2 shRNA was constructed and transfected into EJ cell line with Lipofectamine 2000. We used the gene chip to screen differential expression genes in EJ cells with transfection with EZH2 shRNA compared with untransfected EJ cells. The data were passed to Passway miner serve (（http:// www. biorag. org /passway. htm）) Results We found 436 differentiation genes, in which 257 genes upregulated and 179 genes downregulated. Bioinformation analysis showed that 115 genes locates in the known biological ways, including EGF, p53, proliferation, cell cycle, Notch and Wnt passway. EZH2 target genes such as WNT1, MYT1, CNR1, WT1 were upregulated, some of which are linked to tumor stem cell and cell differentiation. Conclusions The results implied the linking between EZH2 expression and cell proliferation,which is induced by genes concerned cell differentiation. The study focusing on EZH2 regulating genes will promote to clarify its molecular mechanism in oncogenesis.

Key words: bladder cancer, EZH2, gene chip, RNA interference