关键词: 低氧, 兔骨髓间充质干细胞, 增殖, 代谢, ROS

Abstract: Objective To investigate the effect of transition between hypoxia and normoxia on proliferation of rabbit mesenchymal stem cells (rMSCs) in vitro. Methods rMSCs expanded in hypoxia were divided into two groups: L-L group and L-N group. Moreover, rMSCs expanded in normoxia were also divided into two groups: N-N group and N-L group. The L-L and N-L groups were subsequent subcultured in hypoxia, and L-N and N-N groups in normoxia. Growth curves, colony-forming efficiency, glucose and lactate metabolism, and intracellular ROS generation of the four groups were detected. Results In L-L group cell density and colony-forming efficiency were the highest, and intracellular ROS generation was the lowest, whereas reversed results were obtained in N-N group. Cells cultured in hypoxic conditions exhibited higher glucose consumption, lactate production and yield coefficients of lactate from glucose compared to normoxic ones. Conclusions These results suggested that hypoxia could enhance the proliferation of rMSCs in continuous subcultures. There was a potential link between intracellular ROS generation and expansion of rMSCs.

Key words: hypoxia, rabbit mesenchymal stem cell, expansion, metabolism, ROS