基础医学与临床 ›› 2008, Vol. 28 ›› Issue (11): 1138-1141.

• 研究论文 • 上一篇    下一篇

室旁核注射Apelin-13加重大鼠缺血-再灌注胃黏膜细胞凋亡

丁雷 张建福 张咏梅   

  1. 徐州医学院 病理生理学教研室 徐州医学院生理学教研室
  • 收稿日期:2008-01-15 修回日期:2008-03-28 出版日期:2008-11-25 发布日期:2008-11-25
  • 通讯作者: 张建福

Apelin-13 Microinjection into the Paraventricular Nucleus Aggravated Gastric Mucosal Cellular Apoptosis Induced by Gastric Ischemia-Reperfusion in Rats

Lei DING, Jian-fu ZHANG, Yong-mei ZHANG   

  1. Department of Pathophysiology, Xuzhou Medical College Department of Physiology, Xuzhou Medical College
  • Received:2008-01-15 Revised:2008-03-28 Online:2008-11-25 Published:2008-11-25
  • Contact: Jian-fu ZHANG,

摘要: 目的 研究大鼠室旁核(PVN)注射Apelin-13对胃缺血-再灌注(GI-R)的损伤作用及其细胞机制。方法 PVN内微量注射Apelin-13,制备GI-R模型,用免疫组化方法检测胃黏膜细胞的凋亡、增殖及凋亡相关基因BCL-2和BAX的表达。结果 (1) 注射 0.2、1.0和5.0 μg Apelin-13到PVN, GI-R损伤显著加重, 且有剂量-效应依赖关系;(2) 与GI-R组相比,注射Apelin-13能明显增加GI-R后胃黏膜细胞的凋亡,减低胃黏膜细胞的增殖,同时可以增加促凋亡因子BAX蛋白的表达,明显降低抗凋亡因子BCL-2蛋白的表达。结论:室旁核内注射Apelin-13可促进胃黏膜细胞的凋亡、抑制其增殖,对GI-R损伤产生显著的加重作用。

关键词: Apelin-13, 室旁核, 胃缺血-再灌注, 细胞凋亡, BCL-2, BAX

Abstract: Objective To observe effects and mechanism of Apelin-13 microinjection into the hypothalamic paraventricular nucleus on gastric ischemia-reperfusion injury in rats. Methods   After Apelin-13 was microinjected into PVN, the experimental model of GI-R was established by clamping the celiac artery for 30 min and then reperfusing the artery for 1 h. We used immunohistochemistry to detect the gastric mucosal cells apoptosis, proliferation and the expression of BCL-2, BAX. Results (1) Apelin-13 microinjection into the PVN could aggravate GI-R injury in an dose-dependent manner by using 0.2 , 1.0 or 5.0 μg, respectively. (2) Compared with GI-R group, Apelin-13 microinjection into the PVN markedly increased gastric mucosal cellular apoptosis, decreased the proliferation, and promoted the protein expression of BAX, but obviously inhibited the protein expression of BCL-2. Conclusion Apelin-13 microinjection into the PVN could aggravate GI-R injury by promoting gastric mucosal cellular apoptosis and inhibiting proliferation.

Key words: Apelin-13, paraventricular nucleus ( PVN), gastric ischemia-reperfusion (GI-R), cellular apoptosis, BCL-2, BAX