基础医学与临床 ›› 2023, Vol. 43 ›› Issue (1): 152-158.doi: 10.16352/j.issn.1001-6325.2023.01.0152

• 研究论文 • 上一篇    下一篇

右美托咪定减轻缺氧诱导的人神经母细胞瘤细胞系SH-SY5Y损伤

阎晨, 刘涛, 宣斐*   

  1. 新疆医科大学附属肿瘤医院 麻醉与围手术期医学中心, 新疆维吾尔自治区 乌鲁木齐 830000
  • 收稿日期:2022-01-10 修回日期:2022-05-30 发布日期:2022-12-27
  • 通讯作者: *461347647@qq.com

Dexmedetomidine attenuates anoxia- induced injury of human neuroblastoma cell line SH-SY5Y

YAN Chen, LIU Tao, XUAN Fei*   

  1. Center for Anesthesiology and Perioperative Medicine, Cancer Hospital Affiliated to Xinjiang Medical University, Urumqi 830000, China
  • Received:2022-01-10 Revised:2022-05-30 Published:2022-12-27
  • Contact: *461347647@qq.com

摘要: 目的 研究右美托咪定(DEX)在缺氧环境下对人神经母细胞瘤细胞系SH-SY5Y的保护作用及其机制。方法 将SH-SY5Y细胞分为对照组、缺氧组(终浓度为1.2 mmol/L CoCl2处理24 h)和DEX干预组(终浓度为1.2 mmol/L CoCl2和10 μmol/L DEX处理24 h)。显微镜观察细胞形态;MTT比色法、TUNEL染色法和annexin V-FITC/PI染色法检测细胞活性和细胞凋亡;Western blot检测cleaved caspase-9、cleaved caspase-3、p75NTR和p-NF-κB蛋白表达。结果 与对照组比较,缺氧组细胞的胞体变小(P<0.01)、细胞活性降低(P<0.01)、凋亡增加(P<0.01),细胞中cleaved caspase-9、cleaved caspase-3、p75NTR和p-NF-κB蛋白表达水平均明显增加(P<0.01)。DEX干预能显著缓解缺氧组的上述变化(P<0.01)。结论 DEX可能通过抑制p75NTR表达和NF-κB活性缓解缺氧导致的SH-SY5Y细胞损伤。

关键词: 右美托咪定, SH-SY5Y细胞, p75NTR, 缺氧

Abstract: Objective To study the protective effect of dexmedetomidine (DEX) on human neuroblastoma cell line SH-SY5Y under anoxia condition and its specific mechanism. Methods SH-SY5Y cells were divided into control group, anoxia group (treated with final concentration of 1.2 mmol/L CoCl2 for 24 h) and DEX intervention group (treated with final concentration of 1.2 mmol/L CoCl2 and 10 μmol/L DEX for 24 h). Cell morphology was observed by microscope; Cell viability and apoptosis were detected by MTT assay, TUNEL staining and annexin V-FITC/PI staining; The expression levels of cleaved caspase-9, cleaved caspase-3, p75NTR and p-NF-κB proteins were detected by Western blot. Results Compared with control group, the cell size in anoxia group was shrinked(P<0.01). Cell viability was decreased (P<0.01). The apoptosis rate was increased (P<0.01). The expression levels of cleaved caspase-9, cleaved caspase-3, p75NTR and p-NF-κB proteins was all significantly increased (P<0.01). DEX intervention could significantly alleviate the above changes in anoxia group (P<0.01). Conclusions Alleviation of anoxia-induced SH-SY5Y cell injury is potentially mediatd by inhibiting p75NTR expression and NF-κB activity.

Key words: dexmedetomidine, SH-SY5Y cell, p75NTR, anoxia

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