基础医学与临床 ›› 2022, Vol. 42 ›› Issue (12): 1867-1872.doi: 10.16352/j.issn.1001-6325.2022.12.1867

• 研究论文 • 上一篇    下一篇

白藜芦醇减轻脂多糖诱导的小鼠心脏损伤

郑玲, 陈立, 王月*   

  1. 湖北文理学院附属医院 襄阳市中心医院, 湖北 襄阳 441000
  • 收稿日期:2021-11-18 修回日期:2022-05-09 出版日期:2022-12-05 发布日期:2022-11-23
  • 通讯作者: * 450787056@qq.com
  • 基金资助:
    湖北省卫生健康委员会中医药科研项目(ZY2021M052)

Resveratrol alleviates LPS-induced heart injury in mouse models

ZHENG Ling, CHEN Li, WANG Yue*   

  1. Xiangyang Central Hospital,Affiliated Hospital of Hubei University of Arts and Science,Xiangyang 441000,China
  • Received:2021-11-18 Revised:2022-05-09 Online:2022-12-05 Published:2022-11-23
  • Contact: * 450787056@qq.com

摘要: 目的 研究白藜芦醇(RES)可否减轻脂多糖(LPS)诱导的小鼠心脏损伤。方法 将小鼠随机分为对照组、LPS组(腹腔注射LPS 7.5 mg/kg)、RES低(20 mg/kg)和高剂量(60 mg/kg)干预组。超声检测心功能;心脏组织切片HE及TUNEL染色观察小鼠心脏组织结构及细胞凋亡;Western blot检测凋亡相关蛋白Bax和Bcl-2的表达;微板法检测血清中CK-MB和LDH水平;ELISA法检测血清中促炎细胞因子IL-1β、IL-6及TNF-α水平;用试剂盒检测心脏组织匀浆中SOD活力和MDA含量。结果 与对照组相比,LPS组小鼠心功能下降(P<0.05);组织结构改变(P<0.05);CK-MB、LDH、IL-1β、IL-6及TNF-α释放增加(P<0.05);SOD活性下降、MDA含量增加(P<0.05);同时心肌凋亡细胞数量增多及Bax与Bcl-2比值升高(P<0.05),RES干预可明显逆转这些改变(P<0.05),且呈剂量依赖性。结论 RES可通过抗炎、抗氧化及抗凋亡作用减轻LPS诱导的心脏损伤。

关键词: 白藜芦醇, 心功能障碍, 凋亡, 炎性反应, 氧化应激

Abstract: Objective To investigate whether resveratrol (RES) can alleviate lipopolysaccharide (LPS)-induced heart damage in mice. Methods Mice were randomly divided into control group, LPS group(intraperitoneal LPS 7.5 mg/kg) and RES low(20 mg/kg) and high-dose(60 mg/kg) intervention groups. The cardiac function was examined by ultrosound method; heart tissue sections was microscopied with HE staining for morphological change and apoptosis of heart tissue; Western blot was used to examine the expression of the apoptosis-related proteins Bax and Bcl-2. Microplate assay was used to detect CK-MB and LDH in serum; ELISA was performed to detect the proinflammatory cytokines IL-1β, IL-6 and TNF-α levels in serum and a commercially available kit was used to detect SOD viability and MDA content in heart tissue homogenates. Results Compared with the control group, LPS group mice showed a decreased cardiac function(P<0.05) and morphological change of tissue(P<0.05), significant increase of CK-MB, LDH, IL-1, IL-6, TNF-α release(P<0.05), decreased SOD activity(P<0.05), increased MDA content(P<0.05), increased myocardial apoptotic cells and Bax to Bcl-2 ratio(P<0.05) were recorded. The RES intervention significantly reversed these changes (P<0.05), with a dose-dependent manner. Conclusions RES alleviates LPS-induced cardiac damage through an anti-inflammatory,antioxidant and anti-apoptotic mechanism.

Key words: resveratrol, cardiac dysfunction, apoptosis, inflammation, oxidative stress

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