红景天苷负向调控TACC2表达抑制NSCLC细胞增殖及上皮间充质转化

doi:10.16352/j.issn.1001-6325.2022.11.1681

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (11): 1681-1689.doi: 10.16352/j.issn.1001-6325.2022.11.1681

红景天苷负向调控TACC2表达抑制NSCLC细胞增殖及上皮间充质转化

何邵波^1*, 罗甫花², 蒋传命¹, 刘选梅¹

1.邵阳学院医学检验学院, 湖南邵阳 422000;
2.邵阳学院附属第一医院, 湖南邵阳 422000

收稿日期:2021-10-18 修回日期:2022-02-18 出版日期:2022-11-05 发布日期:2022-11-01
通讯作者: ^* heshaobo-001@163.com
基金资助:
湖南省教育厅优秀青年项目(15B220)

Salidroside inhibits the proliferation and epithelial mesenchymal transformation of NSCLC cell by negatively regulation of TACC2 expression

HE Shao-bo^1*, LUO Fu-hua², JIANG Chuan-ming¹, LIU Xuan-mei¹

1. School of Medical Laboratory, Shaoyang University, Shaoyang 422000;
2. the First Affiliated Hospital, Shaoyang University,Shaoyang 422000, China

Received:2021-10-18 Revised:2022-02-18 Online:2022-11-05 Published:2022-11-01
Contact: ^* heshaobo-001@163.com

摘要/Abstract

摘要： 目的探讨红景天苷(SAL)对人非小细胞肺癌(NSCLC)细胞系增殖、凋亡以及上皮间充质转化(EMT)的影响,并明确其作用的分子机制。方法收集22例肺癌患者的癌组织及癌旁组织;并体外培养肺癌细胞系(A549、H358、H1299、H1650);RT-qPCR分别检测肺癌组织以及肺癌细胞系中转化酸性卷曲螺旋蛋白2(TACC2)的表达。不同浓度SAL处理细胞(A549、H1299)后,流式细胞测量术、CCK-8法分别检测细胞凋亡、增殖;Western blot检测细胞(A549、H1299)内E-cadherin、N-cadherin、TACC2表达以及p38磷酸化水平。转染pcDNA3.0-TACC2质粒后,检测过表达TACC2对细胞增殖、凋亡水平以及EMT的影响。结果肺癌组织中TACC2的相对表达量显著高于癌旁组织,肺癌细胞系TACC2表达水平显著高于人胚肺成纤维细胞系(HFL-1)(P＜0.05)。不同浓度SAL处理后,细胞增殖水平显著降低,凋亡率显著上升(P＜0.05)。SAL能够显著降低A549以及H1299细胞内TACC2的表达水平,细胞内E-cadherin表达上升,N-cadherin表达下降。转染pcDNA3.0-TACC2质粒可显著减弱SAL对A549、H1299细胞增殖以及EMT的抑制作用(P＜0.05)。此外,不同浓度SAL处理后,细胞内p38/MAPK磷酸化水平显著降低(P＜0.05)。结论 SAL能够负向调控TACC2表达进而抑制NSCLC细胞的增殖以及EMT过程,该作用可能与p38/MAPK信号通路的抑制有关。

关键词: 红景天苷, 转化酸性卷曲螺旋蛋白2(TACC2), 非小细胞肺癌, 增殖, 上皮间充质转化

Abstract: Objective To investigate the effect of salidroside (SAL) on the proliferation, apoptosis and epithelial mesenchymal transformation (EMT) of non-small cell lung cancer (NSCLC) cell line and to clarify the molecular mechanism. Methods The cancer tissues from 22 lung cancer patients and adjecent tissues were collected, and the different lung cancer cell lines (A549, H358, H1299, H1650) were cultured in vitro. RT-qPCR was used to detect the expression of transforming acidic coiled-coid protein 2(TACC2) in cancer tissues and the lung cancer cell lines. After cells (A549 and H1299) were treated with SAL at different concentrations, the apoptosis and proliferation rates were analyzed by flow cytometry and CCK-8, respectively. The expression of E-cadherin, N-cadherin, TACC2 and the phosphorylation level of P38/MAPK in NSCLC cell were detected by Western blot. Plasmid (pcDNA3.0-TACC2) was transfected into A549 and H1299 cell to detect the effects of TACC2 over-expression on the levels of apoptosis, proliferation and EMT. Results The relative expression of TACC2 in lung cancer tissue significantly increased as compared with adjecent tissues.Meanwhile, the expression of TACC2 in NSCLC cell was also markedly higher than human lung fibroblasts cell line(HFL-1)(P＜0.05). Different concentrations of SAL can significantly inhibit the proliferation and increase the apoptosis of cells(P＜0.05). Western blot results showed that the expression of TACC2 and N-cadherin was significantly down-regulated, while E-cadherin expression was up-regulated in A549 and H1299 cells after treatment with SAL. Transfection with pcDNA3.0-TACC2 plasmid could significantly reduced the inhibited effect of SAL on the proliferation and EMT of A549 and H1299 cells (P＜0.05). In addition, the phosphorylation of intracellular p38/MAPK was significantly reduced after treatment with various SAL concentrations (P＜0.05). Conclusions SAL can inhibit the levels of proliferation and EMT in NSCLC cell by negatively regulates the expression of TACC2, which may be related to the inhibition of p38/MAPK signaling pathway.

Key words: salidroside, transforming acidic coiled-coil protein 2(TACC2), non-small cell lung cancer, proliferation, epithelial mesenchymal transformation

中图分类号:

R734

何邵波, 罗甫花, 蒋传命, 刘选梅. 红景天苷负向调控TACC2表达抑制NSCLC细胞增殖及上皮间充质转化[J]. 基础医学与临床, 2022, 42(11): 1681-1689.

HE Shao-bo, LUO Fu-hua, JIANG Chuan-ming, LIU Xuan-mei. Salidroside inhibits the proliferation and epithelial mesenchymal transformation of NSCLC cell by negatively regulation of TACC2 expression[J]. Basic & Clinical Medicine, 2022, 42(11): 1681-1689.

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红景天苷负向调控TACC2表达抑制NSCLC细胞增殖及上皮间充质转化

Salidroside inhibits the proliferation and epithelial mesenchymal transformation of NSCLC cell by negatively regulation of TACC2 expression

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