基础医学与临床 ›› 2022, Vol. 42 ›› Issue (11): 1675-1680.doi: 10.16352/j.issn.1001-6325.2022.11.1675

• 研究论文 • 上一篇    下一篇

卵巢癌组织中差异lncRNAs的筛选及其lncRNA ZEB2-AS1的表达分析

董辉1, 王婷婷2, 马晓红2, 马盛宗2, 丁永慧3*   

  1. 宁夏医科大学总医院 1.医学科学研究院; 2.临床医学院; 3.妇科,宁夏 银川 750004
  • 收稿日期:2021-09-16 修回日期:2022-04-07 出版日期:2022-11-05 发布日期:2022-11-01
  • 通讯作者: * 13995105311@163.com
  • 基金资助:
    宁夏自然科学基金(2019AAC03201)

Screening of differential lncRNAs and expression analysis of lncRNA ZEB2-AS1 in ovarian cancer tissues

DONG Hui1, WANG Ting-ting2, MA Xiao-hong2, MA Sheng-zong2, DING Yong-hui3*   

  1. 1. Institute of Medical Sciences; 2. School of Clinical Medicine; 3. Department of Gynecology, General Hospital of Ningxia Medical University, Yinchuan 750004, China
  • Received:2021-09-16 Revised:2022-04-07 Online:2022-11-05 Published:2022-11-01
  • Contact: * 13995105311@163.com

摘要: 目的 通过表达谱芯片筛选卵巢癌(OC)有价值的核心长链非编码RNAs(lncRNAs)分子,并进行表达验证和分析。方法 收集宁夏医科大学总医院2018年1月至2019年6月OC组织36份,正常卵巢组织22份,以表达谱芯片检测差异表达lncRNAs和mRNAs分子,以real-time PCR验证lncRNAs表达,原位杂交验证关键核心lncRNA的表达,生物信息学分析lncRNA可能的功能。结果 相比于正常组织,癌组织中共有4 982个差异表达的lncRNA,以锌指E盒结合同源盒蛋白2-AS1(ZEB2-AS1)的高表达最为显著(P<0.05);ZEB2-AS1在肝癌、结肠癌和肾癌等多个癌组织中过表达;表达水平与肿瘤大小、病理分级、病理分型及临床分期均有相关性(P<0.05),且高表达导致总生存期和无病生存期降低(P<0.05)。ZEB2-AS1具有多个microRNA结合位点,与相应邻近基因ZEB2的表达情况一致。结论 ZEB2-AS1在卵巢癌中异常表达,提示ZEB2-AS1具有重要的研究价值。

关键词: 卵巢癌, lncRNA, 锌指E盒结合同源盒蛋白2-AS1(ZEB2-AS1)

Abstract: Objective To screen the valuable core(long non-coding RNAs,lncRNAs) from the tissue of ovarian cancer (OC) through expression profiling chip, and carry out expression verification and analysis. Methods Thirty-six tissue samples of ovarian cancer and of 22 normal ovaries were collected from January 2018 to June 2019 in the General Hospital of Ningxia Medical University and then expression profile chips were used to detect differentially expressed lncRNAs and mRNAs molecules, while real-time PCR was to verify the expression of lncRNAs. The expression of key core lncRNAs were measured by in situ hybridization, and the potential functions of lncRNAs were evaluated by bioinformatics. Results Compared with normal tissues, 4 982 different lncRNAs fragments were found in cancer tissues. The high expression of zinc finger E-box binding homeobox protein 2-AS1 (ZEB2-AS1) was the most significant one (P<0.05); ZEB2-AS1 was over-expressed in multiple cancer tissues including liver cancer, colon cancer and kidney cancer;The expression level was correlated with tumor size, pathological grade, pathological type and clinical stage (P<0.05).The high expression led to a decrease in overall survival and disease-free survival rate(P<0.05). ZEB2-AS1 had microRNA binding sites, which was in agreement with the expression of the corresponding neighboring gene ZEB2. Conclusions The abnormal expression of ZEB2-AS1 in OC suggests potential research value in future.

Key words: ovarian cancer, lncRNA, zinc finger E-box binding homeobox protein 2-AS1(ZEB2-AS1)

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