基础医学与临床 ›› 2022, Vol. 42 ›› Issue (10): 1555-1560.doi: 10.16352/j.issn.1001-6325.2022.10.1555

• 研究论文 • 上一篇    下一篇

土荆皮乙酸诱导人前列腺癌细胞系DU145凋亡

刘冬梅1, 徐强1, 李峰2*   

  1. 1.南通市肿瘤医院 病理科,江苏 南通 226006;
    2.南通大学附属医院 检验科, 江苏 南通 226001
  • 收稿日期:2021-10-28 修回日期:2022-01-28 出版日期:2022-10-05 发布日期:2022-09-23
  • 通讯作者: * 540870553@qq.com
  • 基金资助:
    南通市科技项目(JC2020027)

Pseudolaric acid B induces apoptosis of prostate cancer cell line DU145

LIU Dong-mei1, XU Qiang1, LI Feng2*   

  1. 1. Department of Pathology, Nantong Cancer Hospital, Nantong 226006;
    2. Department of Laboratory Medicine, the Affiliated Hospital of Nantong University, Nantong 226001, China
  • Received:2021-10-28 Revised:2022-01-28 Online:2022-10-05 Published:2022-09-23
  • Contact: * 540870553@qq.com

摘要: 目的 研究土荆皮乙酸(PAB)诱导人前列腺癌细胞系DU145细胞凋亡,并初步探讨其作用机制。方法 以0、5、10、20和40 μmol/L的PAB分别处理细胞24、48、72和96 h;CCK8法检测细胞活性;流式细胞测量术annexin V-FITC/PI双染和TUNEL法观察细胞凋亡;划痕实验和Transwell小室分别检测迁移和侵袭;Western blot检测细胞中Bcl-2、Bax蛋白及 PTEN、AKT、p-AKT蛋白的表达。结果 随着PAB浓度增加和作用时间的延长,DU145细胞增殖活力明显降低(P<0.01)。与对照组相比,PAB组细胞凋亡率、Bax和PTEN蛋白表达量显著增加,而细胞划痕愈合率、穿膜细胞数、Bcl-2以及p-AKT 蛋白表达量均显著减少(P<0.01)。GW9662显著降低PAB对DU145的促凋亡的作用。结论 PAB可能通过PTEN/AKT信号通路抑制前列腺癌DU145细胞增殖、侵袭和诱导细胞凋亡。

关键词: 前列腺癌, 凋亡, 土荆皮乙酸

Abstract: Objective To investigate the effect of pseudolaric acid B (PAB) on apoptosis of prostate cancer cell line DU145 and the potential mechanism. Methods The cells were treated with 0, 5, 10, 20 and 40 μmol/L PAB for 24, 48, 72 and 96 hours respectively;the CCK8 method was used to detect cell proliferation,flow cytometry annexin V-FITC/PI double staining and TUNEL method was used to detect cell apoptosis after the treatment of PAB. Scratch test and transwell chamber were used to detect cell migration and invasion. Western blot was used to detect the protein expressions of Bcl-2, Bax, PTEN, AKT and p-AKT in the cells. Results With the increase of PAB concentration and time of incubation, the cell viability of DU145 cells was significantly reduced (P<0.01). Compared with the control group, the average cell apoptosis rate, Bax and PTEN protein expression in the PAB group were significantly increased, while the cell scratch healing rate, the number of transmembrane cells, Bcl-2 and p-AKT protein expression were significantly reduced(P<0.01). GW9662 significantly reversed the pro-apoptotic effect of PAB on DU145. Conclusions PAB may inhibit the proliferation and invasion of prostate cancer DU145 cells and induce cell apoptosis through the PTEN/AKT signaling pathway.

Key words: prostate cancer, apoptosis, pseudolaric acid B

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