基础医学与临床 ›› 2022, Vol. 42 ›› Issue (9): 1362-1366.doi: 10.16352/j.issn.1001-6325.2022.09.1362

• 研究论文 • 上一篇    下一篇

雷公藤多苷减轻干燥综合征模型小鼠炎性反应

刘珈言, 梅蓉, 杨礼凤, 赵文彬, 韩成龙, 周慧, 李二龙*   

  1. 四川大学华西医院 皮肤性病科, 四川 成都 610041
  • 收稿日期:2021-06-28 修回日期:2021-12-31 出版日期:2022-09-05 发布日期:2022-09-02
  • 通讯作者: h223sc@163.com
  • 基金资助:
    四川省科技计划(2020YFS0164)

Tripterygium wilfordii glycosides reduce inflammation response in Sjgren's syndrome of mouse models

LIU Jia-yan, MEI Rong, YANG Li-feng, ZHAO Wen-bin, HAN Cheng-long, ZHOU Hui, LI Er-long*   

  1. Department of Dermatology & Venerology , West China Hospital, Sichuan University, Chengdu 610041, China
  • Received:2021-06-28 Revised:2021-12-31 Online:2022-09-05 Published:2022-09-02

摘要: 目的 探讨雷公藤多苷(TWPS)对干燥综合征(SS)模型小鼠炎性反应的影响。方法 将小鼠按照随机分数法分为对照组、干燥综合征模型组、硫酸羟氯喹组(20 mg/kg硫酸羟氯喹灌胃)、TWPS低、中、高剂量组(10、20、30 mg/kg灌胃)、TWPS+Wortmanin(PI3K/Akt/eNOS信号通路抑制剂)组(30 mg/kg TWPS灌胃并经颈静脉注射0.6 mg/kg Wortmannin),每组各12只。记录唾液流率、饮水量、全血黏度和血浆黏度;酶联免疫吸附实验检测血清IL-6、IL-17、IgG、eNOS;Western blot检测PI3K/Akt/eNOS通路相关蛋白。结果 模型组较对照组唾液流率下降,饮水量增加(P<0.05);TWPS低、中、高剂量组较模型组唾液流率增加,饮水量下降(P<0.05);TWPS+Wortmanin组较TWPS低、中、高剂量组唾液流率下降,饮水量增加(P<0.05)。模型组较对照组全血和血浆黏度增加;TWPS低、中、高剂量组全血及血浆黏度较模型组降低(P<0.05);TWPS+Wortmanin组全血及血浆黏度较TWPS低、中、高剂量组增加(P<0.05)。模型组血清IL-6、IL-17、IgG水平较对照组增加,eNOS水平较对照组下降(P<0.05);TWPS低、中、高剂量组血清IL-6、IL-17、IgG水平较模型组下降,eNOS水平较模型组增加(P<0.05);TWPS+Wortmanin组血清IL-6、IL-17、IgG水平较TWPS低、中、高剂量组增加,eNOS水平较TWPS低、中、高剂量组下降(P<0.05)。模型组较对照组p-PI3K/PI3K、 p-Akt/Akt、p-eNOS/eNOS水平下降(P<0.05);TWPS低、中、高剂量组较模型组p-PI3K/PI3K、 p-Akt/Akt、p-eNOS/eNOS水平增加(P<0.05);TWPS+Wortmanin组较TWPS低、中、高剂量组p-PI3K/PI3K、 p-Akt/Akt、p-eNOS/eNOS水平下降(P<0.05)。 结论TWPS能够缓解SS模型小鼠炎性反应,改善其症状。

关键词: 唾液流率, 饮水量, 全血黏度, 血浆黏度, PI3K/Akt/eNOS通路

Abstract: Objective To investigate the effect of Tripterygium wilfordii polyglycosides(TWPS) on inflammatory response found in mouse models with Sjgren's syndrome(SS). Methods Mice were randomly divided into control group, Sjgren's syndrome model group, hydroxychloroquine sulfate treatment group (20 mg/kg hydroxychloroquine sulfate intragastric administration), TWPS low-dose, medium-dose and high-dose groups (10, 20, 30 mg/kg TWPS), TWPS + Wortmanin (PI3K/Akt/eNOS signaling pathway inhibitor) group (30 mg/kg TWPS and 0.6 mg/kg Wortmannin viajugular vein).Saliva flow rate, water intake, whole blood viscosity and plasma viscosity were recorded. Serum IL-6, IL-17, IgG and eNOS were detected by ELISA. Western blot was used to detect PI3K/Akt/eNOS pathway related proteins. Results Compared with control group, the saliva flow rate of the model group decreased and the quantity of drinking water increased(P<0.05). Compared with model group, saliva flow rate increased and water consumption decreased in low, medium and high dose TWPS groups, which alleviated the change of model group(P<0.05). Compared with the low, medium and high dose TWPS +Wortmanin groups, saliva flow rate decreased and water intake increased (P<0.05). The whole blood and plasma viscosity of model group increased compared with control group(P<0.05). The whole blood and plasma viscosity of TWPS low-dose, medium-dose and high-dose groups were lower than that of model group(P<0.05). The whole blood and plasma viscosity of TWPS+Wortmanin group was higher than that of low, medium and high dose TWPS groups(P<0.05). Compared with the control group, the levels of serum IL-6, IL-17 and IgG in model group were increased, while the level of eNOS was decreased(P<0.05). The serum levels of IL-6, IL-17 and IgG in TWPS low-dose, medium-dose and high-dose groups were lower than those in model group, while the levels of eNOS were higher than those in model group(P<0.05). The serum level of IL-6, IL-17 and IgG in TWPS +Wortmanin group increased as compared to those in low, medium and high dose TWPS +Wortmanin group, while the level of eNOS decreased as compared to those in low, medium and high dose TWPS +Wortmanin group(P<0.05). The level of p-PI3K/PI3K, p-Akt/Akt and p-ENOS /eNOS in model group was lower than those in control group(P<0.05). Compared with model group, p-PI3K/PI3K, p-Akt/Akt and p-ENOS/eNOS levels of TWPS in low, medium and high dose groups all increased(P<0.05); The level of p-PI3K/PI3K, p-Akt/Akt and p-ENOS /eNOS in TWPS +Wortmanin group was lower than those in low, medium and high dose TWPS groups(P<0.05). Conclusions TWPS alleviates inflammatory response and improves symptoms of mouse models with Sjgren's syndrome.

Key words: saliva flow rate, water consumption, whole blood viscosity, plasma viscosity, PI3K/Akt/eNOS pathway

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