基础医学与临床 ›› 2022, Vol. 42 ›› Issue (2): 215-220.doi: 10.16352/j.issn.1001-6325.2022.02.002

• 研究论文 • 上一篇    下一篇

放射治疗后宫颈癌组织内差异表达基因鉴定

白静1#*, 刘娜2,3,4#, 丁力1, 雷丽1, 倪瑞1, 闫少春2*, 邵国2,3,4*   

  1. 1.包头市肿瘤医院 放射治疗科,内蒙古 包头 014030;
    2.包头医学院 内蒙古自治区低氧转化医学重点实验室,内蒙古 包头 014060;
    3.首都医科大学 宣武医院 低氧适应转化医学北京重点实验室,北京 100053;
    4.包头医学院第二附属医院 内蒙古自治区消化疾病研究所 检验科,内蒙古 包头 014030
  • 收稿日期:2020-11-13 修回日期:2021-06-08 出版日期:2022-02-05 发布日期:2022-01-24
  • 通讯作者: * crystal_bj@sohu.com; yanshaochunbtyxy@163.com; shao_guo_china@163.com
  • 作者简介:#对本文有相同贡献
  • 基金资助:
    国家自然科学基金(81660307,82060337);内蒙古自然科学基金(2018MS08052,2020MS08010);包头医学院基金(BYJJ-YF201717)

Identification of differentially expressed genes in cervical carcinoma after radiotherapy

BAI Jing1#*, LIU Na2,3,4#, DING Li1, LEI Li1, NI Rui1, YAN Shao-chun2*, SHAO Guo2,3,4*   

  1. 1. Department of Radiptherapy, Baotou Tumor Hospital,Baotou 014030;
    2. Inner Mongolia Key laboratory of Hypoxic Translational Medicine,Baotou Medical College,Baotou 014060;
    3. Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine, Xuanwu Hospital,Capital Medical University,Beijing 100053;
    4. Institute of Digestive Diseases of Inner Mongolia Autonomous Region, Department of Laboratory Medicine, the Second Affiliated Hospital of Baotou Medical College,Baotou 014030,China
  • Received:2020-11-13 Revised:2021-06-08 Online:2022-02-05 Published:2022-01-24
  • Contact: * crystal_bj@sohu.com; yanshaochunbtyxy@163.com; shao_guo_china@163.com

摘要: 目的 研究放射治疗对宫颈癌组织基因表达的影响,探讨差异表达基因对放射治疗敏感及在抗拒中的作用。方法 患者接受相同的放疗模式:全盆腔外照射总剂量45戈瑞(Gy),共25次(45 Gy/25 f),腔内近距离内照射每次剂量5~6 Gy,共治疗5~6次。分别在近距离放射治疗前、近距离放射治疗中、近距离放射治疗结束时留取标本。高通量分析近距离照射前,照射中和照射后基因表达的情况;real-time PCR 及Western blot验证与放射敏感和耐受相关的基因。结果 生物信息学显示DNA损伤基因表达增加(P<0.05),real-time PCR 及Western blot分析DNA损伤相关基因GADD45表达较治疗前增加(P<0.05)。结论 放射治疗可引起众多宫颈癌组织基因表达变化,其中GADD45表达增加可能在放射治疗中起到重要作用。

关键词: 宫颈癌, 放射治疗, 生物信息学

Abstract: Objective To study the effect of radiotherapy on gene expression in cervical cancer tissues and to find the role of differentially expressed genes in sensitivity and resistance to radiotherapy. Methods The patients all received the same radiotherapy as follows: total pelvic external radiation 45 Gy/25 f,intracavitary brachytherapy 5-6 Gy/f with total dosage as 5-6 f.The specimens were collected before treatment and by the end of treatment.High-throughput analysis of gene expression was performed before, during and after irradiation; real-time PCR and Western blot was applied to identify the genes related to radiation sensitivity and tolerance. Results Bioinformatics analysis showed an increased expression of DNA damage genes.The expression of DNA damage related genes GADD45 was increased as found by real-time PCR and Western blot. Conclusions Radiotherapy changes the gene expression in cervical cancer tissues and the increase of GADD45 expression may play an important role in radiotherapy.

Key words: cervical cancer, radiotherapy, bioinformatics

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