蛇菰多糖降低实验性肝损伤大鼠肝脏BDNF和TrkB的表达

doi:10.16352/j.issn.1001-6325.2022.01.009

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (1): 62-67.doi: 10.16352/j.issn.1001-6325.2022.01.009

蛇菰多糖降低实验性肝损伤大鼠肝脏BDNF和TrkB的表达

谢雅^1,2, 陈颖², 夏德尧², 唐鲜娥^1,2, 王凤杰^2,3, 陈显兵^1,2*

1.湖北民族大学附属民大医院病理科, 湖北恩施 445000;
2.湖北民族大学医学部, 湖北恩施 445000;
3.湖北民族大学科技学院, 湖北恩施 445000

收稿日期:2020-11-27 修回日期:2021-05-10 出版日期:2022-01-05 发布日期:2022-01-05
通讯作者: ^* 653388390@qq.com
基金资助:
国家自然科学基金(81660672);湖北省教育厅科学研究计划(B2020361);湖北民族大学青年科研基金(MY2018Q016);恩施州科技局项目(JCY2019000033)

Balanophora polysaccharide decreases the expression of BDNF and TrkB in liver tissues of rat with experimental liver injury

XIE Ya^1,2, CHEN Ying², XIA De-yao², TANG Xian-e^1,2, WANG Feng-jie^2,3, CHEN Xian-bing^1,2*

1. Department of Pathology, the Affiliated Hospital, Hubei Minzu University, Enshi 445000;
2. Department of Medicine, 3. Institute of Science and Technology, Hubei Minzu University, Enshi 445000, China

Received:2020-11-27 Revised:2021-05-10 Online:2022-01-05 Published:2022-01-05
Contact: ^* 653388390@qq.com

摘要/Abstract

摘要： 目的蛇菰多糖(BPS)对D-半乳糖(D-gal)所致实验性肝损伤大鼠的肝功能及肝组织内BDNF、TrkB蛋白和凋亡相关蛋白表达的影响。方法将大鼠随机分为对照组(control),D-gal组(皮下注射D-半乳糖200 mg/kg),BPS低(D-gal+BPS-L)、中(D-gal+BPS-M)、高(D-gal+BPS-H)剂量组,分别每天灌胃50、100和200 mg/kg BPS,共6周。心脏取血检测血清谷丙转氨酶(ALT)和谷草转氨酶(AST)水平;HE染色法观察肝组织形态;免疫组织化学染色检测BDNF和TrkB的定位;Western blot检测BDNF、TrkB、Bcl-2、caspase-3和Bax蛋白表达;ELISA测定肝组织内丙醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果与对照组相比,D-gal组肝细胞水肿、点状坏死;血清ALT和AST水平升高(P＜0.05),SOD活性降低、MDA含量升高(P＜0.05);Bax、caspase-3、BDNF、TrkB表达增加(P＜0.05),Bcl-2表达降低(P＜0.05);与D-gal组相比,BPS各剂量组肝细胞变性、坏死明显减轻;血清ALT和AST水平降低(P＜0.05);SOD活性升高、MDA含量降低(P＜0.05);促凋亡蛋白Bax和caspase-3表达降低,抗凋亡蛋白Bcl-2表达增加(P＜0.05);BDNF 和TrkB的表达降低(P＜0.05)。结论 BPS对D-gal所致实验性肝损伤有一定的保护作用,其机制可能与降低肝组织内BDNF和TrkB的表达、减轻肝细胞凋亡有关。

关键词: 蛇菰多糖, 肝损伤, 凋亡, BDNF/TrkB

Abstract: Objective To study the effects of Balanophora polysaccharides (BPS) on the expression of BDNF, TrkB and other apoptosis-related proteins in rats with experimental liver injury induced by D-gal. Methods Rats were randomly divided into control group, D-gal group (200 mg/kg of hypodermic D- galactose), groups with BPS of low dosage (BPS-L), medium dosage (BPS-M) and high dosage (BPS-H), which were treated with intragastric administration by 50, 100 and 200 mg/kg BPS per day, respectively. After six weeks, serum alanine transaminase(ALT) and aspartate aminotransferase(AST) were measured. HE staining was used to observe liver tissue morphology. The localization of BDNF and TrkB was determined by immuno-histochemical staining. Western blot was used to detect BDNF, TrkB, Bcl-2, caspase-3 and Bax protein expression. ELISA was used to determine the malondialdehyde(MDA) content and superoxide dismutase(SOD) activity in liver tissues. Results Compared with the control group, the hepatocytes showed edema and punctate necrosis in D-gal group, with increased serum level of ALT and AST(P＜0.05). The results also showed decreased SOD activity and increased MDA content (P＜0.05), increased Bax, caspase-3, BDNF, TrkB expression (P＜0.05) and decreased Bcl-2 expression (P＜0.05). Compared to D-gal group, the hepatocyte degeneration and necrosis were obviously alleviated in BPS groups with each dose accompanied by decreased serum ALT and AST levels (P＜0.05), increased SOD activity and decreased MDA content (P＜0.05), reduced Bax and caspase-3 expression of pro-apoptotic proteins, increased expression Bcl-2 anti-apoptotic proteins (P＜0.05) and decreased expression of BDNF and TrkB (P＜0.05). Conclusions Balanophora polysaccharide had a protective effect on experimental liver injury caused by D-gal. The mechanism may be related to the decrease of the expression of BDNF and TrkB followed by inhibition of hepatocyte apoptosis.

Key words: Balanophora polysaccharide, liver injury, apoptosis, BDNF/TrkB

中图分类号:

R285.5

谢雅, 陈颖, 夏德尧, 唐鲜娥, 王凤杰, 陈显兵. 蛇菰多糖降低实验性肝损伤大鼠肝脏BDNF和TrkB的表达[J]. 基础医学与临床, 2022, 42(1): 62-67.

XIE Ya, CHEN Ying, XIA De-yao, TANG Xian-e, WANG Feng-jie, CHEN Xian-bing. Balanophora polysaccharide decreases the expression of BDNF and TrkB in liver tissues of rat with experimental liver injury[J]. Basic & Clinical Medicine, 2022, 42(1): 62-67.

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蛇菰多糖降低实验性肝损伤大鼠肝脏BDNF和TrkB的表达

Balanophora polysaccharide decreases the expression of BDNF and TrkB in liver tissues of rat with experimental liver injury

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