OBJECTIVE To develop the limited standard and supporting detection methods of commonly used pesticides in Lycium barbarum L. (L. barbarum). METHODS In previous research, the “the Principle of Conversion of Limit Standards of Traditional Chinese Medicine in <GB 2763 national food safety standard-maximum residue limits for pesticides in food>” have been drafted. In this research, the L. barbarum was used as the research object to study the conversion scope, risk assessment, detection methods and conversion judgment principle. RESULTS A method for the determination of 13 pesticide residues in the L. barbarum was established by using acetonitrile extraction and HLB column purification. And the GC-MS/MS and LC-MS/MS technology were used to detect the pesticide residues. In addition, the maximum residue limit of 13 pesticides in L. barbarum was finally formulated by sample determination. CONCLUSION The specific implementation steps are provided for the conversion of GB 2763 standard to traditional Chinese medicine standard in this study, and it is of great significance for promoting the connection between planting and circulation supervision of L. barbarum.

OBJECTIVE To screen the commonly used pesticides in Dendrobium officinale to understand their pesticide residues, and explore the establishment of a method for determining the transformation of pesticide residues in Dendrobium officinale and the development of limit standards in accordance with the requirements of GB 2763 Food Safety Standards for Maximum Residue Limits of Pesticides in Food and the Chinese Pharmacopoeia. METHODS LC-MS/MS methods were used to screen and risk assess 6 transformation pesticides in 53 batches of Dendrobium officinale, and the pesticide registration status and the maximum residue limit standards for Dendrobium officinale (dry) in GB 2763 were used to determine the indicators for the transformation of pesticides. RESULTS The pesticides in 53 batches of Dendrobium officinale were analyzed. The transformation pesticide were confirmed in accordance with GB 2763 and the requirements of the Chinese Pharmacopoeia 2020 Edition, and a method for determining the transformation of pesticide residues in Dendrobium officinale was established, along with the development of limit standards. CONCLUSION This study screened 40 commonly used pesticides and 6 transformation in 53 batches of Dendrobium officinale, established a method for determining the transformation of pesticide residues in accordance with GB 2763, and developed limit standards. This study is helpful for understanding the risk of pesticide residues in Dendrobium officinale, regulating the use of pesticides during its cultivation process, and improving its quality standards.

OBJECTIVE To establish a method for the transformation of some commonly used pesticide limit standards in Chrysanthemi Flos based on ‘GB 2763 National Food Safety Standard-Maximum Residue Limits for Pesticides in Food’. METHODS The indexes of Chrysanthemi Flos (dry) in GB 2763 were sorted out, and the quasi-transformation pesticides were determined as pymetrozine, imidacloprid, boscalid and fluazinam according to the transformation principle. The UPLC-MS/MS method was used to establish the transformation method of Chrysanthemi Flos, and the methodological verification was carried out. RESULTS The four pesticides had good linear relationship in the range of 1-50 ng·mL^-1, the correlation coefficients were 0.999 9, the average recovery was 77.9%-95.5%, and the RSD was 1.7%-6.8%. The overall detection rate of the samples was 60%, and the pesticide with the highest detection rate was imidacloprid. The maximum detectable amount was 1 mg·kg^-1 (imidacloprid), and the results did not exceed the maximum residue limit specified in GB 2763. The risk assessment system of pesticide residues in traditional Chinese medicine was used to evaluate the chronic risk of the four pesticide limit values to be transformed. The results showed that the chronic risk of fluazinam was greater than 1, and the chronic risk quotients of the other three pesticides were less than 1. Therefore, fluazinam is not converted temporarily, and only pymetrozine, imidacloprid and boscalid are converted. CONCLUSION The three limit standards of pymetrozine, imidacloprid and boscalid in Chrysanthemi Flos were transformed. The established limit standard transformation method is simple and accurate, which is of great significance for standardizing the use of pesticides and medicinal safety in the process of chrysanthemum planting.

OBJECTIVE To establish a method for simultaneous determination of registered pesticides, prohibited pesticides, and proposed new prohibited pesticide residues in Fritillaria Medicinal Herbs, and propose reference limits. METHODS The detection method for 95 pesticide residues in Fritillaria Medicinal Herbs was established using GC-MS/MS and LC-MS/MS. The prepared samples were extracted with acetonitrile and then purified with the QuEChERs method. A total of 70 batches of samples were detected by the established method. RESULTS According to the GB 2763 conversion guidelines, imidacloprid and avermectin in Fritillaria Medicinal Herbs should be converted to the maximum residue limit (MRL) standards. Additionally, reference limit values are proposed for the 18 prohibited pesticides that were previously suggested for addition. CONCLUSION The study of Fritillaria Medicinal Herbs aims to offer guidance and inspiration for the transformation of traditional Chinese medicine standards and the expansion of the list of banned pesticides. It also provides a reference for the regulatory policies and technological advancements related to pesticide residue management in Fritillaria Medicinal Herbs and other traditional Chinese medicines.

OBJECTIVE To carry out screening of pesticide residues in Panax notoginseng for the pesticide residues commonly used in Panax notoginseng,understand the pesticide residues situation and carry out the related risk assenssment study. METHODS GC-MS/MS and LC-MS/MS methods were used to establish detection methods for commonly used pesticides in Panax notoginseng, as well as those regulated by laws and regulations; the methods were used to conduct a comprehensive screening and risk assessment of the collected Panax notoginseng samples; and the indexes of the transformed pesticides were confirmed in accordance with the pesticide residue limits of Panax notoginseng in the GB 2763 standard. RESULTS In this study, the residue determination methods for about 40 commonly used pesticides and pesticides regulated by regulations in Panax notoginseng were successfully established, and the corresponding limits were set according to the GB 2763 standard. Eight pesticides including tebuconazole, phenyl ether metronidazole and carbendazim were finally recognized as transformed pesticide indicators. CONCLUSION The pesticide residue detection method for Panax notoginseng established in this study is characterized by simple and rapid operation, high specificity and high sensitivity, and the related risk assessment and limit setting provide technical support for the improvement of the quality standard of Panax notoginseng.