Effects of Polysaccharides from Five Kinds of Marine Animals on Transformation Rate of Mouse Lymphocytes and Multiplication Rate of Rat Stertoli Cells in vitro
1.Department of Molecular and Biochemical，Second Military Medical University，Shanghai 200433，China； 2.Shanghai Fisheries University，Shanghai 200090，China
OBJECTIVE To study the effect of crude polysaccharides which were extracted from Rapana venosa,Septifer bilocularis,Turbo cornutus,Natica fortunei and Haliplanella luciae on the transformation rate of mouse lymphocytes and the multiplication rate of rat stertoli cells in vitro.METHODS The transformation rate of mouse lymphocytes and the multiplication rate of rat stertoli cells in vitro were tested with MTT method after the cells were cultured with 100 and 1 000 mg·L-1polysaccharides from five kinds of marine animals respectively.RESULTS The results showed that the polysaccharides from Turbo cornutus(P<0.05),Natica fortunei(low concentration group P<0.05;high concentration group P<0.001),Haliplanella luciae(P<0.001) apparently enhanced the transformation rate of mouse lymphocytes and the multiplication rate of rat stertoli cells in vitro.CONCLUSION The crude polysaccharides from Natica fortunei,Haliplanella luciae and Turbo cornutus can enhance the transformation rate of mouse lymphocytes and the multiplication rate of rat stertoli cells in vitro.
XU Hong-li;GUO Ting-ting;FU Li;ZHNG Jin-peng;FENG Wei-hu;JIO ing-hu.
Effects of Polysaccharides from Five Kinds of Marine Animals on Transformation Rate of Mouse Lymphocytes and Multiplication Rate of Rat Stertoli Cells in vitro[J]. Chinese Pharmaceutical Journal, 2006, 41(11): 839-841
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