目的 改进双黄连含片黄芩苷含量测定中的提取方法。方法 采用正交试验法，以含量为指标，对提取过程所用甲醇浓度、超声提取时间、溶剂用量等多因素多水平进行考察，运用数理统计方法找出影响结果的主要因素及最佳提取方案。结果 甲醇的浓度是影响的主要因素(P＜0.01)，提取方法以甲醇超声30 min为佳，经方法学考察，本法加样回收率99.96% RSD=1.4%，n=6，重现性 RSD=1.3%，n=10。结论 本法准确、灵敏、重现性好。

OBJECTIVE To develop an assay for the determination of baicalin in Shuanghuanglian Hanpian. METHODS Orthogonal exdesign was performed to do multifactor and multilevel test,and the content was used as an objective indicator. RESULTS The concentration of methanol in extractant had the greatest effect on determination.The mean recovery was 99.96%(RSD=1.4%,n=6). CONCLUSION This method is accurate,sensitive and reproductive (RSD=1.3%,n=10).