Table of Content

25 December 2006, Volume 26 Issue 12

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研究论文

Sensitive detection of clock genes in embryoid body derived single cell with nested RT-PCR

2006, 26(12):  1313-1318. 

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Homeobox genes cdx1 and cdx2 lead to reduced malinancy in exophageal adenocarcinoma cells

2006, 26(12):  1319-1323. 

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The stugy of LTB4-BLT signal pathway in DCs

2006, 26(12):  1324-1327. 

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MAPK and NF-κB up-regulated the expression of Toll-like receptor 4 induced by LPS in pulmonary micro-vascular endothelial cell in mouse

2006, 26(12):  1328-1331. 

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Objective To explore the role of MAPK and NF-κB in increase Toll-like receptor 4 ( TLR4 ) expression induced by lipopolysaccharide (LPS ) in pulmonary micro-vascular endothelial cell (PMVEC) in mouse. Methods Isolation and culture mouse PMVEC. PMVEC were exposed to the different concentration LPS at 2h, and 100 μg/L LPS in different time. The concentration of TNF-αin culture supernatant was detected using ELISA. Using PD98059, ERK inhibitor, SB203580, inhibitor for p38 MAPK and PDTC inhibitor for NF-κB pretreated PMVEC. RT-PCR was used to detect the levels of TLR4 and TNF-αmRNA. Western-blot was used to detect the protein expression of nuclear factor-kappa B ( NF-κB ) p65 subunit and ERK / p38 MAPK in nuclear extract after LPS exposure for 6 h respectively. Results At doses of 50~500 ng /mL LPS for 2 h or 100μg / L LPS exposure in different time, the concentration of TNF-αin culture supernatant of PMVEC was increase with both time and dosage dependent ( P <0.01). The levels of TLR4 and TNF-αmRNA in membrane of PMVEC were obviously increase, with the peak at 6h. Above inhibiter decrease the TLR4 and TNF-αmRNA expression separately, and above inhibition effect was increased after simultaneous pretreatment with PD98059 and SB203580. Protein expression of ERK/p38 MAPK and NF-κB in PMVEC induced by LPS were down-regulation by pretreatment with PD98059, SB203580 and PDTC respectively. Conclusion Up-regulation expression of TLR4 in PWVEC of mouse by MAPK and NF-κB pathway induced by LPS.

Cytogenetic analysis of male infertile patnts

2006, 26(12):  1332-1334. 

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Objective To investigate the frequency of chromosome abnormality in male infertility in Chinese. Methods Karyotypes were analyzed by chromosome G-banding in 415 infertile patients with idiopathic azoospermia or severe oligozoospermia. Results 44(10.6%) of subjects were found to have chromosome abnormality. Conclusion The results of present study indicated that chromosome abnormality is one of the frequent genetic causes for male infertility, suggesting that it is essential to screen chromosome abnormality during diagnosis of male infertility as well as before in vitro assisted fertilization by intracytoplamic injection.

Study of thr effect on colon carcinoma cells SW480 of WTp53 gene combined with TK/GCV and CD/5-FC system

2006, 26(12):  1340-1344. 

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Effect of PI3K/Akt signal passageway on renal tubulointerstitial fibrosis in unilateral ureteral obstruction rats

2006, 26(12):  1345-1349. 

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Influence on basic electrical rhythms and interstitial cells of Cajal by blockade of c-kit in the murine small intestine

2006, 26(12):  1350-1353. 

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Molecular mechanism study of cyclooxygenase-/JAK2/Stat3 signaling pathway in vascular smooth muscle cells of rat

2006, 26(12):  1354-1358. 

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Study of the combination of neuroprotective agents on the apoptosis of neurons and the expression of caspase-3 of focal ischemia in rats

2006, 26(12):  1359-1362. 

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Abstract: Objective To investigate whether the protective effect of therapy with different combined neuroprotective agents was better than that of single agent. Methods Use suture occlusion technique,the right middle cerebral artery in rats was occluded. All animals were randomly assigned before surgery to one of the following groups: FDP-treated group,MK-801-treated group,NAC-treated group,combination-treated group and contral group.The apoptosis of neurons and the expression of caspase-3 were observed after 6h and 24h of cerebral ischemia.Results After 6h and 24h of cerebral ischemia,both the apoptosis of neurons and the expression of caspase-3 in the combination-treated group was distinctly decreased than the single agent group (P<0.05).Conclusion Combined use of neuroprotective agents may have be more effective than that of single agent in this modle.

The effect of H2O2 on cultured meanocyte

2006, 26(12):  1363-1366. 

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Hair keratin artificial material cell immunity and cell toxicant research

2006, 26(12):  1367-1370. 

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研究短文

Changes of immune function and relationship between the xhanges and estradion and cortisol in early human pregnancy

2006, 26(12):  1371-1372. 

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The change of cardiasc structure and fuction MMP-2,MMP-9expression after GCV thrombosis

2006, 26(12):  1373-1374. 

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Expression of prepro-orexin and its recetors in the hypothalamus of dietary obesity rats

2006, 26(12):  1375-1376. 

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The expression of basic fibroblast growth factor and mRNAin asthmatic rats and the effect of deamethasone

2006, 26(12):  1377-1378. 

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The effect of prestorage leucoreduction on the cytokines levels in blood storage

2006, 26(12):  1379-1380. 

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Effect of neuron apoptosis on autonomic neuropathy in diabetic rats

2006, 26(12):  1381-1382. 

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短篇综述

The relationship between S100A4 & tumorigenesis progression and metastasis

2006, 26(12):  1383-1388. 

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Advancement of recritment of eosinophil in the pathogenesis of asthma

2006, 26(12):  1389-1392. 

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High-Density lipoprotrins and the imptovement of endothelial function

2006, 26(12):  1397-1401. 

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协和大查房

Mass in vena cava inferior and edema after myomectomy

2006, 26(12):  1402-1408. 

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