Basic & Clinical Medicine

The effects of electical stimulation of paravrntricular nucleus on gastric mucosal cells in relieved gastric ischemia-reperfusion injury of rats

2007, 27(3): 238-242.

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Objective To observe the effects of electrical stimulation of paraventricular nucleus (PVN) on gastric mucosal cellular apoptosis，proliferation，and expression of BCL-2，BAX induced by gastric ischemia-reperfusion (GI-R), investigate the cellular mechanisms of protection of PVN on GI-R injury . Methods After electrical stimulation of PVN, the experimental model of GI-R were established by clamping the celiac artery for 30 min and then reperfusing the artery for 30 min，1 h，3 h，or 6 h respectively. We used immunohistochemistry to detect the gastric mucosal cells apoptosis, proliferation and the expression of BCL-2,BAX. Results Compared with GI-R group，the electrical stimulation of PVN markedly decreased gastric mucosal cellular apoptosis，increased the proliferation，and promoted the protein expression of BCL-2，but markedly inhibited the protein expression of BAX at 30 min,1 h, 3 h after reperfusion respectively. Conclusion The protective effect of PVN on GI-R injury is associated with up-regulation of expression of BCL-2 and down-regulation expression of BAX，and then inhibiting gastric mucosal cellular apoptosis and promoting proliferation induced by GI-R injury.

Experimental study of treating thrombocytopenic purpura and the mechanism in SD rat models with CL2MDP-loaded gelatin particles

2007, 27(3): 243-247.

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Objective To explore whether Cl2MDP-loaded gelatin particles can induce the depletion of macrophage or can depress the immunity of macrophage in SD rat models of immune thrombocytopenic purpura (ITP) to treat the ITP rats. Methods SD rats were intravenously injected with 150 μl of anti-platelet serum once per 24 hr to induce the ITP model. The platelet counts were persistently less than 50×109/L in these models. After intravenous injection of a group dose of Cl2MDP-gelatin particles, the platelet counts of these models were examined at the time of 4 hr, 24 hr, 48 hr, 72 hr and 96 hr, respectively. The pathological changes of macrophages in spleen were observed by electron microscope. Results Cl2MDP-loaded gelatin particles can increase significantly the platelet counts of ITP models from less than 50×109/L to mean 180×109/L in 24 hr after injection, and can keep the platelet level through whole experimenting process. The pathological changes of macrophages were seen under electron microscope, such as degeneration, necrosis and so on. Furthermore, rats that were pre-treated with Cl2MDP-loaded gelatin particles can evade the decrease of platelet counts when they were injected anti-platelet serum. Conclusion Cl2MDP-loaded gelatin particles can promptly, effectively restore platelet counts of ITP models to physiologically level, in a dose dependent manner. So, the targeting therapy of drug-loaded gelatin particles offers a new approach to treat ITP, and this strategy is worthy of further studies.

The influence of quercetin and X-Ray on the collagen sythesis of cultured le;oid fibrolast

2007, 27(3): 248-253.

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Objective To study the influence of quedcetin on the collagen synthesis of cultured fibroblast, and to explore the mechanism of the effect. Methods The inhibitory effects of quercetin and radiation on fibroblast proliferation were assayed using MTT assay. Collagen synthesis was detected by hydroxyproline colorimetric analysis. Immunocytochemical staining method was used to investigate the expression of collagen I and III of fibroblasts. The mRNA expression of type I, type III collagens and TGFβ-1 were assayed by using reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR technique. Results Keloid fibroblast cell proliferation and collagen synthesis of fibroblasts could be inhibited by quercetin in a dose-dependent manner. Significant inhibition was observed by the treatment with quercetin and radiation together. Immunocytochemical staining indicated the IOD of type I and III collagen protein was down-regulated by quercetin and radiation. Both collagen I and collagen III gene in the quercetin groups showed a significantly decreased mRNA expression compared with that in the untreated group，especially in the group treated with both quercetin and X-ray. Procollagen gene expression could be inhibited to prohibit type I and III protein syntheses of fibroblsts by inhibiting gene transcription (p<0.05), particularly type I procollagen gene (p<0.01). The mRNA level of TGFβ-1 gene could also be down-regulated by quecertin, which was probably the mechanism of the drug effect. Conclusions Quercetin associated with radiation can considerably inhibit collagen synthesis of keloid-derived fibroblast by decreasing the mRNA expression in vitro. The result is mainly accomplished by the down-regulated effect on TGF β-1 gene level.

Peroxisome proliferator-activated receptor-γand retinoid X recepror-α expression predicts digestive system carcinaoma cell response to piogliatone and 9-cis-retioic acid treatment

2007, 27(3): 254-258.

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Objective To investigate the relationship between the expression of PPARγ and RXRα and the inhibitory effect of pioglizatone , ligands of PPARγ and 9-cis-RA, ligand of RXRα on growth of human digestive carcinoma cell lines(MGC803, LOVO and SMMC7721) in vitro. Methods The Antiproliferative effect was evaluated by cell viability using MTT assay. The mRNA expression of PPARγ and RXRα was semi-quantified by RT-PCR. Results The growth of MGC803 and SMMC7721 cells was significantly inhibited when treated with PGZ and a combination of PGZ and 9-cis-RA , respectively, but no significant inhibitory effect was observed in LOVO cells. The combination group has more inhibitory effect in MGC803 and SMMC7721 cells than PGZ alone (P＜0.01) . PPARγ and RXRα mRNA were both expressed in MGC803，SMMC7721 and LOVO cells, and the expressive level ranked MGC803＞SMMC7721＞LOVO. PGZ significantly up-regulated the expression of PPARγ in MGC803 and LOVO cells, the expression of PPARγ was higher in combination group than PGZ alone(P<0.05). The expression of RXRα in MGC803 and LOVO cells treated with 9-cis-RA was both significantly up-regulated, but has no significant difference between combination group and 9-cis-RA alone. Conclusions It is of necessity that the expression of PPARγ and RXRα in MGC803, SMMC7721 and LOVO cell lines predicts them response to pioglizatone and 9-cis-retinoic acid treatment, the inhibitory effect was different in these three kinds of cell lines, it maybe related to ligands-mediated signal pathway.

Effect of lichemic postconditioning on myocardial ischemic/reperfusion injury in ratsin vitro

2007, 27(3): 259-262.

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Objective Observing the effects of ischemic postconditioning on ischemia/reperfusion injury in rat heart in vitro and reseaerching the mechanisms elementarily. Methods The wistar rat hearts were put in the Langendorff equipment, and the myocardial ischemia/reperfusion and the ischemic postconditioning model in vitro were made. Observing the changes of maximal rate of the pressure increase and decrease (±dp/dtmax) , and left ventricular end diastolic press（LVEDP）of heart. Colorimetry method was used to assay the lactate dehydrogenase (LDH) of fluid, methy lnedioxyamphetamine (MDA) and superoxide dismutase (SOD) activity of cardiac muscle; western blotting method was used to detect endothelial nitric oxide synthase (eNOS) and phosphorylated extracellular-regulated kinases (ERK1/2) expression；RT-PCR method was used to test CYP2J3 mRNA expression of heart. Results Compared with group IR, the value of ±dp/dtmax was increased（P<0.01）, LVEDP and LDH level was decreased（P<0.05） in group IPo in reperfusion stage. The content of MDA in group IR was higher than in group CON and IPo（P<0.01）. The activity of SOD in group IR was lower than IPo（P<0.01）, but it was almost equal to group CON .The expression of eNOS and phosphorylated ERK1/2 in group IR was higher not only than group CON but also than IPo. The CYP2J3 mRNA expression of heart in group IPo was evidently higher than group CON and IR. Conclusions Ischemic postconditioning can improve the myocardial dysfunction and cell injury caused by ischemia/reperfusion in rat heart in vitro, maybe through activation of SOD and increasing elimination of oxygen-derived free radicals. CYP2J3/EET system involved in protective effection probably.

Postmortem studies on serotonin transporters in the brain of parkinson's disease patients

2007, 27(3): 263-266.

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Objective To study serotonin transporters (SERT) in postmortem human brains of Parkinson's disease (PD). Methods Immunoautoradiography was used to reveal SERT distribution in postmortem PD brains. Results In comparison with healthy group, SERT decreased in dorsal raphe nucleus, substantia nigra and striatum in PD brains. Quantitative analysis revealed that 25.9%、31.1%、27.2% and 24.7% of labeling intensity of SERT decreased in putamen, caudate nucleus, substantia nigra and ventrotegmental area as compared with corresponding control respectively. Among the four regions studied of dorsal raphe nucleus,SERT labeling intensity significantly decreased 38.9%, 37.3%, 16.5% and 37.0% of corresponding control respectively in ventral part，dorsal part，caudal part and interfascicular part. Median raphe nucleus did not show the decreased SERT labeling. Conclusions Decreased SERT expression in three regions of postmortem PD brains indicates that a dysfunction of serotonergic raphe system may contribute to the etiology of Parkinson's disease.

Analysis of HLA alleles with psoriatic members in a northern chinese family

2007, 27(3): 267-270.

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Objective To investigate the correlation of HLA class I and II alleles in a Northern Chinese Family members with psoriasis vulgaris. Methods The polymerase chain reaction amplification with sequence-specific oligonucleotide probe (PCR-SSO) method was used to analysis the alleles of HLA-A,B,DRB1 in all the family members. Results HLA-B*13-DRB1* 07 was identified as a high-risk haplotype in the four early-onset psoriatic members (II-2,II-4, III-1,III-5) (age of onset <40y); while HLA-A*11-B*15-DRB1*15 was identified as a susceptible haplotype in the six members in which there were four patients (I-2,II-2,II-4,III-1). Conclusion Several susceptible locus are involved in PsV in this family . Our research demonstrated the positive association of the haplotype 1-13-7 with the early -onset psoriasis vulgaris.It may be due to increase individual's sensitivity to genetic factors ,as well as , environmental factors. The penetrance of the haplotype susceptible to psoriasis was affected by many factors.

Study on the therapeutic effect losartan on ADM and ET-1 in diabetic rats

2007, 27(3): 271-274.

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Objective To investigate the therapeutic effect of Losartan on ADM, ET-1 and their receptors in the kidney of diabetic rats. Methods Experimental diabetes were induced in uninephrectomized rats by STZ. The animals were divided into three groups: group A (control group), group B (diabetic group) and group C (Losartan-treated group). Immunohistochemistry, western bloting, in situ hybridyzation and RT-PCR were used to detect the protein and mRNA expressions of ADM, ADMR, ET-1 and EDNR-A. Results Compared with group A, the expressions of ADM, ADMR, ET-1 and EDNR-A in group B distinctly increased. After Losartan treatment, those debased. Conclusions The expressions of ADM, ET-1 and their receptors may be responsible for the renal protective effect of Losartan in diabetic rats.

Expression of signaling molecule RhoA and phosphorylated myosin light chain in rat liver tissue during hepatic fibrogenesis

2007, 27(3): 275-278.

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Objective To observe the expressive changes of RhoA and phosphorylated myosin light chain in rat liver tissue during hepatic fibrogenesis. Methods The liver histopathological changes were evaluated by hematoxylin and eosin staining and Masson's trichrome method. Western blot was used to determine the expressions of RhoA and p-MLC(Thr18/Ser19), and reverse transcription-polymerase chain reaction (RT-PCR) to determine the expression of RhoA mRNA. Results With the development of hepatic fibrogenesis, the protein expressions of RhoA and p-MLC(Thr18/Ser19) and the mRNA expression of RhoA were significantly increased. RhoA and p-MLC(Thr18/Ser19)correlated with α-SMA positively, respectively. Conclusions Rho/ROCK signaling pathways are changed during hepatic fibrogenesis.

E ffect of AcSDKP on the expression of MMP-1\MMP-2and MMP-9 in cultured cardiac fibroblast

2007, 27(3): 279-283.

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Objective To investigate whether AcSDKP can regulate MMP-2 and MMP-9 activity and MMP-1 expression in cultured rat cardiac fibroblasts mediated by 10％fetal bovine serum and PDGF. Methods The MMP-2 and MMP-9 activity was measured with zymography assays. The MMP-1 expression was detected with western blot. Results 10% fetal bovine serum （FBS） and PDGF stimulated MMP-2 and MMP-9 activity and MMP-1 expression of cardiac fibroblasts；AcSDKP further increased MMP-2 and MMP-9 activity and MMP-1 expression of cardiac fibroblasts induced by 10％ FBS and PDGF。Conclusion AcSDKP can up-regulated MMPs activity or expression of cardiac fibroblasts mediated by PDGF，which is possibly related with the effect of AcSDKP anti-fibrosis.

Effect of low intensive ultrasound on dexamethasone-induced intraocular hypertension in rabbit

2007, 27(3): 284-287.

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Objective To investigate the effect of low-intensity ultrasound on intraocular hypertension and its mechanicsm． Methods　 The intraocular hypertension rabbits were treated with ultrasound .The intraocular pressure(IOP), trabecular meshwork HE staining ,transmission electron microscope, extracellular matrix through immunohistochemistry were observed．　Results　After treatment, IOP was descended and trabecula interspace was wider than the control eye. The expression of collagen I,laminin and fibronectin was lower than control group. Conclusion　Low-intensity ultrasound can decrease IOP. It maybe relate with the change of trabecular meshwork histologic structure .

Expression of survivin and CD44v6 in uterine cervical carcinoma and associated with HPV16/18 infection

2007, 27(3): 288-292.

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Objective To investigate the relationship between expression of survivin and CD44v6 and HPV16/18 infection in uterine cervical carcinogenesis. Methods Using Streptavidin-Peroxidase(S-P) immunohistochemical technique, the authors examined the expression of survivin and CD44v6 in samples. The infection of HPV type 16,18 DNA were determined by PCR. Results There were significant differences for survivin and CD44v6 between carcinomas, CIN and normal cervices（P<0.01,respectively）. Expression of CD44v6 was correlated with tumor grades, clinical stages and lymph node metastasis（P<0.05）, and expression of survivin was associated with tumor grades and lymph node metastasis（P<0.05）. The positive rate of HPV16/18 was significant difference between cervical carcinomas, CIN and normal tissues（P<0.01）and was not associated with tumor grades, clinical stages and lymph node metastasis. The infection of HPV16/18 was associated with the expression of survivin（P<0.05）. Conclusions The abnormal expression of survivin is associated with the infection of HPV16/18. survivin and CD44v6 possibly become useful indexes for the primary screening and prognosis of cervical carcinomas.

The characteristics of gastric myoelectical activity in ohese people

2007, 27(3): 293-297.

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Objective to explore the characteristics of gastric myoelectrical activity induced by meal in obese people. Methods 41 cases of obese subjects were investigated with Digitrapper electrogastrography(EGG) before and after test meal，32 healthy volunteers with normal body mass index were studied as control. Results before meal, the percentage of normal slow waves(N%)in obese group was lower than that in control subjects(55.52±15.7 vs 70.83±14.31, P<0.05); the percentage of bradygastria (B%) and the percentage of arhythmia (AR%) in obese group were higher than those in control group respectively (17.07±10.88 vs 11.46±8.86, 9.70±5.7 vs 5.75±4.14,P<0.05 and P<0.01). ②after meal, N% in the obese was lower than that of the control group (53.19±17.77 vs 64.34±22.63, P<0.05);DP and the percentage of tachygastria(T%) in the obese were higher than those in control group respectively (36.50±4.86 vs 32.24±6.25, 19.93±11.62 vs 12.94 ±11.72,P<0.01 and P<0.05). ③the increase of DP, amplitude and power ratio (PR) in the obese were significantly higher than those in control group (P<0.01 and P<0.05).Conclusion At fasting state and post-meal state, obese people have abnormalities in gastric myoelectrical activity. Abnormal gastric electrical activity and DP'increase after meal may be electrophysiological basis of gastric hypermotility in obese people.

Cultiation and identification of mesenchymal stem cells derived from human umbilical cord blood and is clinical signification

2007, 27(3): 298-301.

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Objective The mononuclear cells (MNCs) were cultivated and expanded into mesenchymal stem cells (MSCs) from human umbilical cord blood, and the purpose of this study was to explore the biological characteristics and induced differentiation ability in vitro. Methods Human umbilical cord blood samples were obtained sterilely with preservative-free heparin and the mononuclear cells were isolated from it, then plated the MNCs in 25-mm culture flasks containing DMEM/F12 medium. MSCs were obtained. The morphology was observed under microscope. Nissl body staining was used, The passage 2, 4, 7 of the expanded MSCs were induced to differentiate to neuron-like cells. The expressions of nestin and neuron-specific enolase (NSE) on the treated cells were detected by immunocytochemical method. Results Nissl body staining was positive; Nestin expression was found in (51.2±3.2) of the second, (34.6±2.7) of the fifth , (11.3±3.3) of the seventh passage of MSCs; NSE expression was found in (11.4±2.3) of the second, (21.78±3.1) of the fifth , (40.7±3.4) of the seventh passage of MSCs. Conclusion Cord blood MSCs possess some features of neural stem cells, and have the capacity to differentiate into neuron-like cells under proper conditions.

Protective effects of human ELAFINgene recombinant plasmid transfection to airway epithelium integrity

2007, 27(3): 302-305.

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Objective To explore the molecule mechanism of protective effects of transfecting human elafin gene recombinant plasmid to NCI-H292 .Methods Through constructing eukaryotic expression vector pEGFP-C1-elafin and transfecting it into NCI-H292 cells ,then co-incubation with polymorphonuclear granulocyte(PMN) and stimulating these cells with lipopolysaccharide(LPS).Afater 24hour,Western bolt and MTT technique were respectively performed to detect expression of ZO-1 in cells and adhesion capability of cells with collagen.Results After stimulation with LPS，Western blot experiment showed that the 220-kD zonula occludens-1(ZO-1) protein in transfecting pEGFP-C1 NCI-H292 cells extracts were obviously decreased，but were still present in transfecting pEGFP-C1-elafin NCI-H292 cells extracts.And MTT technique showed that compared with transfecting pEGFP-C1-elafin cells,adhesion capability of transfecting pEGFP-C1 cells with collagen were obviously decreased.Conclusions Transfection of recombinant human elafin gene into airway epithelial cells could maintain airway epithelium and enhance the capability of airway to inflammatory injury.

Expression changes of catecholamine metabolism related enzymes in the lung tissues of a rat acte PE model

2007, 27(3): 306-309.

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Objective To study the expression changes of catecholamine metabolism related enzymes COMT and MAO in the lung tissues of a rat acute pulmonary embolism (PE) model. Methods We constructed a rat acute PE model. The blood samples were collected by intracardiac puncture at different time points 1h, 8h, 24h and 48h. The mRNA level changes of COMT, MAO-A and MAO-B were identified by semi-quantitative RT-PCR, and the protein level changes of COMT, MAO-A and MAO-B were validated by western blot methods. The serum concentrations of catecholamine were determined by HPLC methods. Results At different time points, the mRNA and protein expression levels of COMT, MAO-A and MAO-B decreased gradually after acute PE. On the contrary, the serum concentrations of epinephrine (EPH) and norepinephrine (NE) increased gradually compared with controls. Conclusion The serum concentrations of catecholamine increased after acute PE because its degradation was suppressed. The study of their expression changes may be helpful to uncover the molecular mechanisms of pulmonary artery hypertension involved in acute PE.

Effects of atorvastatin on expression of ATP-binding cassette transporter transporter AI in THP-1 macrophages

2007, 27(3): 310-313.

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Objectives:To investigate the effection of atorvastatin on expression of ABCA1 （mRNA，protein） and LXRαmRNA in THP-1 macrophages. Methods: Cultured THP-1 cells were induced to form macrophages by PMA for 48 hours. The macrophages were incubated with atorvastatin in different concention for 24 0r 48 hours. We determined the changes of ABCA1mRNA, protein and LXRαmRNA by reverse trancriptase polymerase chain reaction (RT-PCR) and immuno- histochemistry. Results: The expression levels of ABCA1 mRNA（ratio of relative expression 1.21 vs 1.48） and protein as well as LXRαmRNA(0.87 vs1.12) were decreased in THP-1macrophages when cultured with atorvastatin(10μmol/L) for 24h compared with control. They would be lessen further with the cultured time. Conclusions: Atorvastatin inhibit the expression of ABCA1 mRNA and protein as well as LXRαmRNA of THP-1macrophages in vitro.

Effect of demethylating agent on RUNX3 expression and the growth of human hepatocarcinoma cell line HepG2

2007, 27(3): 314-318.

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Objective This study was designed to investingate the effect of 5-Aza-2'-deoxycytidine (5-Aza-CdR)on cell growth and explore the possibility of re-expression of the hypermethylated and silenced RUNX3 gene in the hepatocarcinoma cell line HepG2. Methods The change in expression of the tumor suppressor gene RUNX3 mRNA in cultured HepG2 cells was observed by using RT-PCR before and after 5-Aza-CdR treatment. Activity of cell growth was observed by MTT assay and colony-forming test . The cell cycle was analyzed by flow cytometry . Apoptotic morphology was observed by transmitting electron microscopy. Results The gene was reactivated by two different doses of 5-Aza-CdR treatment in HepG2 cell not expressing RUNX3. The hepatocarcinoma cell line treated with 5-Aza-CdR displayed a slowed growth rate in contrast to the control group. The colony formation rate of HepG2 cell treated with 5-Aza-CdR decreased dramatically (P<0.01). The S phase was arrested and morphosis was changed as induced by 5-Aza-CdR. Conclusion 5-Aza-CdR may effectively cause demethylation and inhibit the growth of the hepatocarcinoma cell line HepG2 by reactivating the RUNX3 gene transcription silenced by aberrant hypermethylation.

Liquid Nutrient Load Test in Assessment of Proximal Stomach Function in Healthy Subjects

2007, 27(3): 319-323.

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Objective: To compare the difference effects on the proximal stomach between rapid (intake rate 100ml/min) and slow (intake rate 15ml/min) LNLT. Methods: Forty healthy subjects received both the rapid and slow LNLTs on two separate days. The caloric density of the nutrient was 1kcal/ml. The TVDRI and MVDRI were recorded, and the corresponding TVPS and MVPS were measured by B-ultrasound. Results: (1) There were positive correlations between TVDRI and TVPS , and between MVDRI and MVPS in both slow and rapid LNLTs. (2) TVDRI and MVPS in slow LNLT were significantly smaller than those in rapid LNLT, but MVDRI was significantly larger in slow LNLT than that in rapid LNLT. (3) TVDRI was much closer to TVPS in slow LNLT than those in rapid LNLT. But MVDRI was much closer to MVPS in rapid LNLT than those in slow LNLT. Conclusions: Both slow and rapid * 通讯作者 LNLTs could be used to evaluate the accommodation to a meal and visceral sensitivity of the proximal stomach. The visceral sensitivity is related to the accommodation function. TVDRI of slow LNLT is more sensitive in estimating the visceral sensitivity of the proximal stomach; MVDRI of rapid LNLT is more accurate in estimating the accommodation of the proximal stomach.

Expression and purification of fusion protein of normal rat brain NT3 geme and antibody preparation

2007, 27(3): 324-328.

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Objective To clone NT-3 gene from normal rat brain and purify its fusion protein and prepare specific high titer antibody so that to provide foundation for further study for peripheral nerve injury.Methods We amplified target gene by RT-PCR and cloned it into the vector of pMD-18T , then analyzed its sequence and compared it with the sequence in GenBank.We subcloned it into pRSET-A vector and introduced it into Escherichia coli BL21.The expression was induced by IPTG,and identified by SDS－PAGE.The fusion protein was purified by niccolum purify kit.We immuned rabbits with immunological adjuvant for specificity antibody preparation. Results We got a 777bp gene segment by RT-PCR.The DNA sequence was identical to rat NT-3 gene sequence in GenBank. It suggested that the target gene was correctly inserted into the vector.A new protein band of about 34 kD appeared on SDS-PAGE after induction of IPTG.A specific high titer antibody of 1:64000 was gained by immuning rabbits with immunological adjuvant.Conclusions The fusion protein encoded by NT-3 gene which was successfully derived from normal rats has been expressed in Escherichia coli and been purified.A specific high titer antibody has been prepared , all this make it possible to do further investigation in reparation of peripheral nerve injury.

primary culture of hippocampal neurons of new-born rats in vitro

2007, 27(3): 329-332.

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Objective To establish the primary culture method of hippocampal neurons of new-born rats in vitro, and observe the morphological characteristics at different developmental and differential stages. Methods After separated from Wistar rats born within 24 hours, the hippocampus was digested and the cells were seeded in sterile flask. 1h later, the neurons were transferred and re-seeded on cover glasses, which were coated with poly-L-lysine previously. The neurons were identified by immunocytochemistry with polyclonal antibody to neuron specific enolase (NSE). The morphological characteristic was observed under phase-contrast microscope at different time after seeding. Results A large number of hippocampal neurons began to adhere to the cover glasses 12-24 hours later. They showed different shapes-shuttle, triangle, pyramidal, or nonregular after clinging to the plate. Their processes connected into nets and were different in length and thickness, usually with one of them longer than the others. They were well developed on the 7th-10th day and could survive as long as 28 days after seeding. Immunocytochemistry of NSE indicated high purity of neurons. Conclusions: The culture method of new-born rat hippocampal neurons in vitro is successful and can be used for further study.

Screening of serum biomarkers in leukemia bu SELDI technique:a preliminary study

2007, 27(3): 333-336.

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Objective The aim of this study was to screen serum biomarkers in patients with acute leukemia by using surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technique. Methods Proteomic spectra were generated by mass spectrometry in 158, including 44 acute lymphocyte leukemia patients and 36 acute myeloid leukemia, 78 healthy control. 158 spectra obtained were used to train and develop a decision tree classification algorithm. Results A total of 7 distinguished proteomic peaks were detected in acute lymphocyte leukemia and were used to build a proteomic pattern. The results yielded a sensitivity of 88.6％（39/44）、specificity of 82.9％（29/35）；A total of 8 distinguished proteomic peaks were detected in acute myeloid leukemia，7 peaks were used to build a proteomic pattern. The results yielded a sensitivity of 86.1％（31/36）、specificity of 95.3％（41/43）. Conclusion SELDI-TOF-MS offers a unique platform for the proteomic detection of acute leukemia. It also offers a noninvasive method to further study the proteomic changs in the development and progression of leukemia.

Clinical manifestions and fiangnosis of POEMS syndrime:clinical analusis on 36 cases

2007, 27(3): 337-341.

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Object：To analyze the clinical manifestations and the criteria for the diagnosis of POEMS syndrome. Methods: The clinical characteristics of 36 cases of POEMS syndrome were retrospectively reviewed and compared with literature. Results: Besides of the typical characteristics of polyneuropathy (100%), organomegaly (92%), endocrinopathy (86%), monoclonal plasmaproliferative disorder (100%) and skin changes (86%), the patients of POEMS syndrome also have other important features, including extravascular volume overload (97%), papilledema (57%) and bone lesions (25%). Furthermore, 25% of POEMS syndrome patients have co-existent Castleman disease. Conclusions: To make the diagnosis of POEMS syndrome, major and minor criteria should be required. The former includes polyneuropathy and monoclonal plasmaproliferative disorder and the latter includes osteosclerotic bone lesions, Castleman disease, papilledema, organomegaly, edema or serous cavity effusion, endocrinopathy and skin changes.

Comparative study on the sensitivinty of two bioassays for quality control of culture media used in vitro fertilization

2007, 27(3): 342-343.

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The survival and morhometric analysis of spinal motoneurons culturing in vitro

2007, 27(3): 344-345.

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The roles mesenchymal stem cells playde in hematological diseases

2007, 27(3): 346-352.

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Mesenchymal stem cells (MSCs) are one of the most fascinating areas of cell biology today. It is well known that MSCs are capable of self-renewal and multi-lineage differentiation, and possess powerful immunoregulatory function. Progresses have been made in the area of the application of MSCs in the therapy of some related diseases. Rencently, knowledge has been accumulated to clarity MSCs' roles in etiology and progression of certain diseases. This review focuses on the relationship between MSCs and some hematological diseases.

Privacy and confidentiality of patients

2007, 27(3): 358-360.

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Information that is disclosed in the context of a professional relationship must be held confidential. It is an important ethical principle, and also physicians' conventional obligations that respect for the privacy of patient and ensure the confidentiality of the information. This article discussed the situation of unjustifiable disclosure and justifiable infringement of privacy and confidentialility as well

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