Basic & Clinical Medicine

Effects of protein kinase C on proliferation, differentiation and sox9 expression of rat growth plate chondrocytes in vitro

Yu-hua Ji Yao-ying Zeng Qiu-hong Ji

2007, 27(9): 961-965.

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To investigate the effects of protein kinase C (PKC) on proliferation, extracelluar matrix synthesis and transcriptional factor SOX9 (SRY-related high mobility group-box gene 9) expression of rat growth plate chondrocytes in vitro. Rat costochondral growth plate chondrocytes (RGC) were isolated and cultured. The 1st serum free cultured passage RGCs were treated with 1, 10 and 100nmol/L phorbol 12,13-dibutyrate (PDBu), PKC agonist, cell morphology alterations were observed with inverted microscopy, cell proliferation, COLLAGEN and GAG synthesis were detected by isotope incorporation COLLAGEN TYPEⅡ and AGGRECAN mRNA transcription and SOX9 expression were revealed by RT-PCR and Western blot. 100nmol/L PDBu treatment made the cell morphology of serum free cultured RGC closed to serum group and inhibited proliferation but promoted COLLAGEN and AGGRECAN synthesis, 3H-TdR、3H-proline and 35S-sulfate incorporation of 100nmol/L PDBu group were as 83%, 52.6% and 146.5% as those of serum free control（P<0.05）. COL2A1 ， AGGRECAN gene transcription and SOX9 expression were also enhanced by 100nmol/L PDBu treatment. There were no obvious effects of 1nmol/L and 10nmol/L PDBu on 1st RGC. Our results indicate that activated PKC inhibits growth plate chondrocyte proliferation and promotes differentiation.

Cytokines secretion in splenocytes of mice was promoted by recombinant BCG-EmII/3 vaccine of Echinococcus multilocularis

Wen-gui Li Hong Wang You-ming Zhu

2007, 27(9): 966-969.

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Objective To dynamically observe changes of cytokines of splnocytes in mice by immunization with recombinant BCG-EmII/3 vaccine of Echinococcus multilocularis(Em).Methods BALB/C mice were intranasally vaccinated by the vaccine and killed to get spleen on 0、2、4、6、8 and 10w of immunization respectively, splenocytes were separated to culture by stimulation with EmAg or ConA，their supernatants were gathered to measure level of IL-2,IFN-γ,TNF-α and IL-4 by ELISA Kits. PBS was served as control. Results In the groups of immunization, levels of IL-2,IFN-γ,TNF-α and IL-4 increased obviously on 2～6、2～6、2～10 and 8～10w respectively, reached the highest level on 4、4、8 and 10w respectively. Conclusion TH1 response was induced in mice by rBCG-EmII/3 vaccine on early immunization（2～8w）.

Molecular biology change of intervertebral disk degeneration caused by bilateral zygapophysial joint unstability

Zong-qiang Huang Shang-li Liu Zhao-min Zhen

2007, 27(9): 970-974.

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Objective: To study aggrecan, collagen type I and collagen type II gene expression of intervertebral disk degeneration through destroying bilateral zygapophysial joints of New-Zealand rabbit. Methods 30 male New-Zealand rabbits were randomly divided into operation group on the bone and operation group on the soft tissue. In operation group on the bone, L4 and L5 inferior articular processes were en bloc excised, L5 and L6 superior articular processes were retained. In the operation group on the soft-tissue, Only L3 to L7 paravertebral muscles was stripped. In operation group on the bone, L4-5 and L5-6 intervertebral disks were acted as experimental group; L3-4 and L6-7 acted as self-control group. In the operation on the soft tissue, L4-5 and L5-6 were acted as experimental control group. One、two、four and eight months post-operation, New-Zealand rabbits were killed. Aggrecan, collagen type I and collagen type II gene expression were performed with semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Results Aggrecan and collagen type II mRNA levels had decreased markedly, whereas type I collagen mRNA gradually increased with time. At the same time point, aggrecan, collagen type II gene expression were the least in experiment group, whereas were most in the experiment-control group. Collagen type I showed the contra-tendency. Conclusions Intervertebral disk degeneration can be induced through destroying L4-5 and L5-6 zygapophysial joints of New-Zealand rabbit.Aggrecan, collagen type I and collagen type II gene expression can reflect intervertebral disk degeneration.

Effects of extracellular adenosine triphosphate on apoptosis of human esophageal carcinoma Eca-109 and hepatoma SMMC-7721 cells

Kui Qin Zhong-ning Zhu Lei-ming Ren Jiang-hui Liu

2007, 27(9): 975-980.

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Objective To study the effects of adenosine triphosphate (ATP) on the proliferation of human squamous esophageal carcinoma Eca-109 and hepatoma SMMC-7721 cells in vitro and the underlying mechanism. Methods MTT assay was used to determine the proliferation of tumor cells. The AO/EB double stained cells were observed under fluorescence microscope to observe morphological changes. The effects of ATP on the cell cycle, apoptotic rate and apoptosis-related protein were detected by flow cytometry. Results ATP produced inhibitory effects on Eca-109 and SMMC-7721 cells at the concentration between 0.01~0.3 mmol/L. Exposed to 0.3mmol/L ATP for 72h, some of SMMC-7721 cells displayed morphological changes of apoptosis, but Eca-109 cells did not show the characteristics of apoptosis markedly. There was no significant change in the apoptotic rate and apoptosis-related protein of the two tumor cell lines treated with ATP 0.03, 0.1 and 0.3mmol/L for 72h respectively. The proportion of Eca-109 cells in G0/G1-phase of cell cycle was significantly increased, meanwhile the proportion of Eca-109 cells in S-phase and proliferation index value was significantly decreased by treatment with 0.3mmol/L ATP. Conclusion ATP inhibited Eca-109 cell proliferation by changing the distribution of cell cycle phase, and its mechanism might not related to apoptosis, but for SMMC-7721 cell, the inhibition of cell proliferation induced by ATP was not related to the change in cell cycle.

Effect of Hydralazine on the methylation of APC in cervical carcinoma cell lines

Yin-hong Song Chang-ju Zhang

2007, 27(9): 981-984.

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　Objective　To detect the 5′CpG island methylation of APC( adenomatous polyposis coli) gene promoter region in cervical carcinoma cell lines (HeLa,CaSki,SiHa), and to investigate the possibility of Hydralazine in restoring the expression of APC gene through demethylation. Methods　The CpG island methylation status of APC gene promoter region of the cervical carcinoma cell lines were analyzed using methylated specific polymerase chain reaction (MSP). The mRNA expression profile of APC gene was analyzed by using RT-PCR after Hydralazine treatment. Results　 The CpG island of APC gene was methylated in HeLa and hemimethylated in CaSki but not in SiHa. The mRNA expression of APC gene can be detected after a certain concentration Hydralazine treatment. Conclusion The APC gene non-expression in cervical carcinoma is associated with CpG island methylation in APC gene promoter region. Hydralazine, served as a demethylating agent, enables to restore the expression of APC gene.

Expression Increase of Chymase Gene in Diabetic Hamsters

Ke-xiang Zhao Qian Xiao Yuan Guo

2007, 27(9): 985-990.

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Objective To explore the mechanism that how chymase affected diabetic cardiomyopathy by investigating the gene expression of chymase and the relationship between the gene expression of chymase and the angiotensin II in the cardiomyopathy of streptozotocin-induced diabetic hamasters. Methods Diabetic hamsters was induced by intraperitoneal injection of STZ 40mg/kg once a day for 3 days. After stabilization of diabetic state for 18 weeks, the myocardial ultrastructure was observed with electron microscope and pathologic changes were observed by microscope. Immunohistochemistry was used to measure the levels of expression of type I and type III collagen in diabetic and normal hamster hearts. Level of blood glucose, lipoprotein was determined using biocehemical methods. Apoptosis of cardiomyocyte was measured using TUNEL methods. Radioimmunuoassay method was used to determine the level of angiotensin II. RT-PCR was used to determine chymase gene expression (corrected byβ-actin). Results Comparing with the control group, levels of serum glucose, TG, TC, LDL in DM group were much higher. Concentrations of collagen I, III and angiotensin II ( 95.8±16.0 pg/mg tissue vs 51.1±20.8 pg/mg tissue) in myocardial tissue in DM group were much higher than those in control group. RT-PCR result showed: Comparing with the control group, the mRNA expression of chymase in DM group was promoted significantly (0.810±0.026 vs 0.490±0.087). Conclusion In diabetic hamsters, the gene expression of chymase were much higher than the control group, accompanying higher level of Angiotensin II, higher levels of expression of collagen I and III. This suggests that chymase plays an important role in the diabetic cardiomyopathy by promoting the activity of chymase.

Upregulation of osteopontin expression in renal tubules of diabetic rats

Chen Tian Cui Zhang Huan Guo Ying Xiao Bing Guo Guo-zhong Zhang

2007, 27(9): 991-995.

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Objective To observe dynamically osteopontin(OPN) expression in the renal tubules of streptozotocin(STZ) induced diabetic rats, and to explore the relationship between OPN and angiotensinⅡ(AngⅡ), nuclear transcription factor-κB(NF-κB), and renal injuries. Methods Male SD rats were injected with STZ to induce diabetes mellitus, which were divided into 5 groups randomly and meanwhile there were other 5 age-matched normal control groups. Immunohistochemistry was employed to observe the expression of OPN, AngⅡ, NF-κB and fibronectin(FN) in renal tubules . The OPN and I-κB protein in renal cortex was detected by Western blot methods. Blood glucose, serum creatinine and 24 h urine protein were examined by biochemistry methods. The renal morphology was checked through light microscopy. Results The AngⅡand NF-κB expression from DM day 3, OPN expression from day7, FN from week 2 in renal cortex or tubules were increased compared with control groups, while I-κB protein in renal cortex was gradually decreased since day 3. In DM week 4, there were positive correlations between OPN and AngⅡ, NF-κB, FN and 24h urine protein. Conclusions Our results suggest that an increase of OPN expression in the renal tubules of diabetic rats may be regulated by AngⅡ and NF-κB, and therefore participates the injury mechanisms of renal tubulo-intestitium.

Effects of Matrine on ATP Binding Cassette Transporter A1 and Cholesterol in monocyte Cells

Yi-deng Jiang Hui-ping Zhang Jun Cao Gui-zhong Li Shu-ren Wang

2007, 27(9): 996-1000.

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Objective To study the effect of matrine on ATP binding cassette transporter A1 (ABCA1) expression and cholesterol in monocyte derived foam cells Method The lipid peroxide within cells was measured by TBARS method; The foam cells were examined by oil red O stain. The accumulation of cholesterol in monocyte were measured by fluorescence spectrophotometric method;ABCA1 mRNA and its protein level were determined by RT-PCR and Western blot, respectively. Result In the homonocytes incubated with PMA and low density lipoprotein (LDL), the intracellular accumulated total cholesterol (TC), free cholesterol (FC) , cholesteryl ester (CE) and lipid peroxide increased obviously, and a great percent of foam cells were found by oil red O stain. This was accompanied by a reduction in the membrane content of ABCA1. matrine alter ABCA1 mRNA abundance , indicating that increased ABCA1 transcription , enhanced mRNA level. conclusion The mechanism of anti-artherosclerosis by matrine may be related to reduce cholesterol and to increase the level of ABCA1 expression.

Increased Tau phosphorylation levels and Glucose Synthase Kinase -3β activities in hippocampus of rats with type 1 or type 2 diabetes

Shu-hong Hu Yan Yang Dong-yun Ai Jian-hua Zhang Mu-xun Zhang

2007, 27(9): 1001-1005.

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Objective To investigate the phosphorylation levels of tau in hippocampus of type 1 and type 2 diabetic rats, and its possible mechanism. Methods The models of type 1 (T1DM) and type 2 diabetes (T2DM) were made，and controlled by the same age rats(CTL). The plasma insulin and the plasma glucose levels were determinated by RIA and glucose-oxidase methods, respectivly.The indexes of insulin resistance were calculated by HOMA-IR. Total tau protein and phosphorylation levels (pSer199,pThr212, pSer214, pSer396 and pSer422) of tau were analyzed by Western blot. The activity of glycogen synthase kinase -3β(GSK-3β), a key component of insulin signal transduction pathway and a known tau kinase, in hippocampus of rats was examined by γ32P-ATP and its specific substrate. Results Plasma glucose was significantly higher in T1DM and T2DM group than in CTL group, and the plasma insulin was significantly higher in T2DM group but lower in T1DM group than in CTL group. Insulin resistance, which was calculated by HOMA-IR, was significantly higher in T2DM than in T1DM and CTL group. Tau protein is hyperphosphorylated at several Alzheimer disease(AD)-related phosphorylation sites (Ser199,Thr212 and Ser396). The activity of GSK-3β also increased significantly in the brains of both diabetic models. Conclusions These findings suggest that type 1 and type 2 diabetes increase the probality of AD by downregulation of insulin signal transduction and the consequent upregulation of GSK-3β, which leads to hyperphosphorylation of tau protein.

Effects of T-cell suppression mediated by mesenchymal stem cells in patients with chronic aplastic anemia

Li-hui Liu Zhao Sun Qin Han Li-ping Ye Bing Shi Jian-gang Jin

2007, 27(9): 1006-1010.

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Objectve To compare the effects of T-cell suppression mediated by mesenchymal stem cells (MSC) from normal individuals and chronic aplastic anemia (CAA) patients. Methods MSC were cultured from the bone marrow of 5 healthy volunteers and 10 CAA patients, Morphology, surface markers and the expression of serveral cytokines of MSC were compared, and the effects of T-cell supression were tested in the following assay: phytohemaglutinin (PHA)-primed cultures, mixed lymophocyte reaction (MLR) and cell cycle of T-cell after PHA-primed cultures. Results MSC from normal volunteers and CAA patients were similar in morphology, proliferation and surface markers. The suppression of T-cell proliferation induced by PHA and alloantigens mediated by CAA MSC was significantly lower than that of normal MSC. More T-cells were arrested in G0/G1 phase by normal MSC, while the effects were deficient by CAA MSC. The cytokines TGF-β2, HGF expression were similar in normal MSC and CAA MSC, but TGF-β1, 3, expressed by CAA MSC were reduced comparing with normal MSC. Conclusion Although the morphology, proliferation and cell surface markers of CAA MSC were normal, the T-cell suppression mediated by CAA MSC is deficient, persists indifinitely after immunosuppressive therapy, whether these abnormalitied are relevant to the pathogenesis of aplastic anemia remains to be determined.

mRNA Expression And Promoter Hypermethylation Status of RASSF1A In Lung Carcinoma

Zong-tao Yu Ya-li Yuan Ji-cai Zhang Qiong Gao Jun Lv

2007, 27(9): 1011-1014.

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Objective To investigate promoter methylation , mRNA expression of RASSF1A gene（RAS association domain family 1A）and their correlationship in lung carcinoma. Metholds The promoter methylation status of RASSF1A were detected by methylation-specific PCR（MS-PCR）,mRNA expression level was measured by real-time reverse transcription polymerase chain reaction. Results methylation rate of RASSF1A gene was 53.33%（24/45）in lung carcinoma tissues ,13.04%（3/23）in normal lung tissues（χ2 =10.32 ρ<0.05）. RASSF1A mRNA expressed normally in all 23 normal lung tissues ,whereas, loss expression in the tissues of 28.89%（13/45）lung carcinoma,the mean level of RASSF1A mRNA was significantly lower in lung carcinoma tissues than in normal tissues（t=18.696, ρ<0.05）.However, it was not correlated with clinical data, such as sex, age, pathological type and tumor size. In 24 patients with the aberrant promoter methylation, 13 cases showed loss expression of RASSF1A mRNA. Conclusion Frequency of RASSF1A promoter methylation is apparently higher in lung carcinoma tissues than in normal tissues,the mean level of RASSF1A mRNA expression is also lower in lung carcinoma than in normal tissues. It signify that RASSF1A methylation may contribute to the low expression of RASSF1A and development of lung carcinoma.

Role of P21 protein and XIAP on apoptosis-inducing effect by mevastatin against A549 cell line

Li-ping Guo Jian-min Luo

2007, 27(9): 1015-1020.

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Objective To investigate the role of P21 protein and XIAP on apoptosis-inducing effect by mevastatin against A549 cell line and its mechanisms . Methods The cells proliferation were detected by MTT assay . The cell cycle distribution and apoptosis induction were evaluated with flow cytometer and morphologic electron microscope changes. The mRNA expression of p21 and XIAP was measured with reverse transcription-polymerase chain reaction .The protein expression of p21and XIAP were test with flow cytometer and Western Bolt respectively . Results Mevastatin inhibited A549 cell survival in a time-dependent and concentration-depended manner. Flow cytometry show that mevastatin induced G0/G1 phase arrest and caused apoptosis. Mevastatin did not affect P21 expression at both mRNA and total protein level.Concomitantly,P21 protein localized on cellular membrane decreased.Both mRNA and protein expression in XIAP were down regulated by mevastatin . All these effects were reversed by addition of mevalonate . Conclusions Mevastatin can inhibited proliferation ,induce apoptosis and interfere with cell progression of A549 through mevalonate pathway. Its mechanisms involved in decreasing the expression of XIAP mRNA and protern.Targeting HMG-CoA reductase ,Mevastatin blocks the isoprenylation of p21 protein which affects its anchorage on the cellular membrane.

Changes of Nitric Oxide Synthase Activity in Serum of Atherosclerotic Rabbit

Hui-ying Zhao Bao-hua Xu Xiao-xin Ma

2007, 27(9): 1021-1024.

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Objective: To research the changes and effects of nitric oxide synthase activity in atherosclerotic rabbit models. Methods: Thirty rabbits were divided into 3 groups at random: control group, atherogenic diet group, balloon-injury+ atherogenic diet group. 3 months later underwent pharmacological triggering with Chinese Russell's viper venom and histamine to make the plaque rupture. The lipid concentrations and the levels of nitric oxide (NO), NOS and hydroxy radical (OH) in the serum were obtained at different period, and pathologic changes were observed. Results: In atherogenic diet group and balloon-injury+ atherogenic diet group, the serum lipid concentrations increased markedly, but the levels of NO hadn't difference. The activity of NOS and OH levels were increased obviously after the atherogenic diet. But it reduced after pharmacological triggering and plaque rupture. Cnclusions: In earlier period of atherosclerosis, NOS activity up regulated and endothelium functions were compensated. But, When the atherosclerotic plaques were unstable, the activity of NOS descended obviously and endothelium function go to incompensation.

Single Nucleotide Polymorphism of Human APM1 Detected by Using Allelic 3'-Terminal Specific Primers Real-time PCR

Xian-rang Song Shu-fang Wang Wei-ling Chi

2007, 27(9): 1025-1028.

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Objectives To develop an analyzing technique of single nucleotide polymorphism(SNP) based on non-probe real-time quantitative PCR, and to study the relationship between SNP in human adiponectin (APM1) gene and type 2 diabetes (T2DM). Methods Design primers with specific 3'-terminal nucleotides and perform Real-time PCR using SYBR Green dye. Genotypes were determined by comparing the amplification efficiency of each pair of primer and verified by sequencing. SNPs 45 and 276 in AMP of 20 T2DM, 24 obesity and 28 healthy persons were analyzed with this technique. Results This technique could discriminate genotype as efficient as sequencing. The genotype of APM1 at +45 site was significantly correlated with T2DM (p<0.05). Moreover, the relative risk of T2DM incidence in persons with TT genotype was 3 times of those with GG and GT. On the contrary, obesity was not correlated with SNP at +45 nor +276 sites of APM1 gene. Conclusion Allelic 3'-terminal specific primers real-time PCR could be used to determine SNPs. SNP at +45 sites of APM1 gene correlated with T2DM.

Significance of c-erbB-2 protein expression on the prognosis of gastric carcinoma: a Meta-analysis study

Gui-ping Qin Xi-an Lu Guang-qiang Qi Cheng-yi Qu Jian-fang Liang Shi-ying Li

2007, 27(9): 1029-1032.

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Objective To evaluate the association of c-erbB-2 protein expression with the prognosis of gastric carcinoma. Methods 11 Literatures was analyzed quantitively which published last 15 years on the relationship of c-erbB-2 with prognosis by software RevMan. Results The pooled odds ratio(OR) of 5-year survival rates was 0.69（95%CI：0.54－0．87）for all 11 literatures. The pooled odds ratio(OR) of three literatures from eleven literatures was 0．67（95％CI：0．48－0．94）which 5-year survival rates was determined as final index and OR as associated．For well-differentiated and advanced gastric carcinoma, the pooled odds ratio(OR) of 5－year survival rates were 0．18（95％CI：0．09－0．38）and 0.72(95％CI：0.47－1.09)respectively．Conclusion The association of c-erbB-2 expression with prognosis of gastric carcinoma may be negative．

Tetracycline inhibits Staphylococcus aureus growth in bacterial prostatic tissues of rats

Xiao-juan Wu He Wang Zheng-hong Chen

2007, 27(9): 1033-1037.

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Objective: To probe the methods of experimental research for the drug activities of the rat prostates. Methods: With androgen and Staphylococcus aureus injected into the prostates, the bacterial prostatitis (BP) models and benign prostatic hyperplasia with bacterial prostatitis (BHP-BP) models were developed. The animals were fed with tetracycline and then the drug activities in the serum and the prostatic tissues, the quantities of the bacteria and the histopathlogical changes in the prostateic tissues were detected separately in variant times. Results: After ten days, the histopathlogical changes of the benign hyperplasia and mesenchyma could be found in the prostatic tissue from BHP animals and acute suppurative inflammation could be found in prostatic tissue from the BP group after infected for two days. The drug activities of 22.44mg/g in BP group and 20.26mg/g in BPH-BP group were found until 3.5-4hours and the values were higher than MBC of the tetracycline and the antibacterial activity of the sera at the same time. After 8 to 11 days of treating, the bacteria would not be isolated from the prostates of the animals with BP or BHP-BP and the acute or chronic inflammation histopathlogical change did not be found in the prostates of animals with BP. 30 CFU of bacteria could be found in per gram tissue of prostate and both of the acute and chronic suppurative inflammation could be found in the BP group without treatment at the 28 days afterinfected. Conclusion: The tetracycline activities in the prostates of rats with BP or BPH-BP will be same as or even higher than that of serum and the MBC of the drug. The quantity of sensitive bacteria in prostates will be diminished and disappeared during treatment.

Apoptosis in the gastric mucosa with stress damage

2007, 27(9): 1038-1042.

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Objective To investigate the way of the gastric epithelial cell death with stress damage. Methods Water immersion-restraint stress model were performed with SD rats, Apoptotic cells were quantitated in gastric mucosa by terminal deoxynucleatidyl transferase mediared dUTP nick and labelling (TUNEL) techniques and electronmicroscopy while the bcl-2/bax proteins were detected by immuhistochemical staining. Results Comparing with the control groupe, at 2h after the WRS,the mucosa was damaged seriously, the apoptotic quantities rose significantly（P<0.01） , The expression of Bcl-2 Protein significantly decreased （P<0.05）, while Bax increased significantly （P<0.01）, At 24h after WRS, the apoptotic cells gradually declined but failed to return（P<0.05）, the expression of Bax remained significantly higher （P<0.05）but Bcl-2 return to normal level. The electronmicroscopy showed that in contrast to normal mucosa cells, apoptotic cells changes with varies characteristics.Conclusions The apoptosis was an important way of cell death of the gastric mucosa with stress damage.

Detection of the transplanted cells in rat brains by ISH using probes on Y chromosomes

Na Li Huan-ying Zhao Hui Yang

2007, 27(9): 1043-1047.

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Objective Developing a method to detect the transplanted cells in rat brains. Methods A SRY gene probe on rat Y chromosomes was cloned and labeled. The probe was used in situ hybridzation (ISH) on female rat brains. Results The experiments showed that the probe of SRY gene could catch the male-derived cells exactly in female brains. Conclution Using ISH of male-derived probe can trace the transplanted cells in female rats.

Investigation of the effectiveness of ex vivo expansion of human umbilical cord hematopoietic stem cells

Xin-jian Qu Hui-xia Li Mei-fu Feng Shi-duo Sun

2007, 27(9): 1048-1052.

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Objective To investigate the effectiveness of in vitro expansion of human umbilical cord hematopoietic stem cells (HSCs) and to optimize the culture conditions. Methods HSCs were cocultured with osteoblasts in the three-direction (3D) culture system or condition medium (CM) to study the expansion effect on HSCs. Results In the conditions of cocultured with osteoblasts and added with 15%CM produced an expansion of the HSCs at a higher level , the HSCs were evaluated that expansion 6.46-fold after 7th days incubation while 14.82-fold after 14th days incubation. At the same conditions, the number of colony forming units of granulocyte and macrophage (CFU-GM) and erthroid burst-forming unit (CFU-E) were also significant higher than that of other groups. Conclusions The HSCs were cocultured with osteoblast in 15% CM medium not only to achieve admirable results for in vitro expansion HSCs but also sustain the self-renew capacity.

Significance of Antibodies to Filaggrin in Diagnosis of Rheumatoid Arthritis

Mai-xing Ai Xiao-mei Leng Xiao-feng Zeng

2007, 27(9): 1053-1056.

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Objectives To determine the diagnostic value of antifilaggrin antibodies(AFA) in rheumatoid arthritis(RA) and compare the correlation among AFA，anti-perinuclear factor(APF) and anti-keratin antibodies(AKA). Methods Filaggrin was extracted from human epidermis and partially purified. AFA in 103 cases of RA and 140 cases of controls were detected by western blot. APF and AKA were detected using indirect immunofluorescence. Results AFA test resulted in diagnosis of 35.9% of 103 RA samples, with a specificity of 93.7%. The result was significantly higher than the controls (P<0.001). 26 (70.3%) samples out of 37 AFA positive sera were positive in the AKA test and 31 samples out of 37 AFA positive were positive in APF test. The data show that there is a positive correlation among AFA, APF and AKA tests. Conclusion AFA has diagnostic value in rheumatoid arthritis. Although there is a positive correlation among AFA, APF and AKA, AFA dose not replace APF and AKA tests.

Resection of Mediastinal Tumors by VATS

Feng GE Zhi-yong Wang Zhi-li Cao

2007, 27(9): 1057-1058.

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Acute myocardial infarction model in rats

Zun-qi Liu Lian-qun Cui Yu-sheng Ge

2007, 27(9): 1059-1060.

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Volume-regulated anion channels participate in the proliferation of human gastric carcinoma cells

2007, 27(9): 1061-1062.

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Recognition of MicroRNAs and its-targeting genes

Bao Song Xian-rang Song Jie Liu Ling Wei

2007, 27(9): 1063-1065.

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MicroRNAs (miRNAs) are a recently discovered family of small non-coding RNAs, which exist in eukaryotes and regulate gene expression post-transcriptionally by binding to complementary sites on target mRNAs. MiRNAs play important roles in regulation of cell proliferation, development, apoptosis and cell differentiation. It's predicted that about 1% human known genes encode microRNAs and microRNAs may regulate 10~30%of the genes within the human genome. Here we focus on the knowledge about how to recognize miRNAs and identify their target mRNAs.

Activated signaling pathway in pathogenesis of pancreatic cancer

Xin-yu Ren Zhi-yong Liang Tong-hua Liu

2007, 27(9): 1066-1071.

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The application of molecular biological technique provides the only hope to pancreatic cancer patients for the cancer's resistance to conventional treatment. Clarifying the molecular mechanism in the genesis and development of pancreatic cancer is the first thing to do. Recent researches found that there were different signaling pathways activated in different stages of pancreatic cancer. The important signal pathways included mitosis pathways, growth factor pathways, developing pathways and mucin pathways. This article will review on the relationship between molecular pathways and pancreatic cancer.

Ethics difficult position and its solution of clinical application teaching of medicine student

Asaiguli

2007, 27(9): 1072-1074.

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For medical students clinical observation is an important turning point from theory to clinical practice;full and systematic clinical practice provides student，s with practical clinical experience,enhancing and strengthening the students theoretical knowledge,an extremely important stage of training in independent work.This article is based on the observations of the features of clinical teaching,analyzing contemporary clinical teaching and the extant ethical issues in the students clinical studies，and thus exploring possible solutions．

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