Table of Content

25 July 2008, Volume 28 Issue 7

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专题综述

Doping detection techniques and progress

Yang QIN; You-xuan XU

2008, 28(7):  0-0. 

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In this article, anti-doping techniques were described , including sample preparation, isolation and analysis. On-line solid-phase extraction with column-switching technique and High through rapid analysis were also discussed.

Doping test by LC/MS

Shu-min YANG

2008, 28(7):  0-0. 

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this paper describes some work on the analysis of glucocorticoid、diuretics and some other drugs by LC/MS, which is difficult to get a satisfactory analysis result by GC/MSD.

医学论坛

Effect of exercise on Muscle Fiber Type Transform of Skeletal Muscle

Jun-ping LI Rui-yuan Wang

2008, 28(7):  664-669. 

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Muscle fiber type of skeletal muscle and its expression of the variety of the specialized protein isoform are the structure and molecular basis of the skeletal muscle's function and adaptability. Myosin heavy chain（MHC）isoform, regarded as the factor which determinates whether the type of the skeletal muscle is fast or slow，have become molecular symbol by which we can tell the muscle fiber type and study muscle's adaptability. Exercise may lead to the transform between myosin subtypes. This article makes a general review not only on the relation between myosin heavy chain and muscle fiber type of skeletal muscle, but also on the influences made by different motor patterns' upon the transform of skeletal muscle fiber MHC isoform.

研究论文

Expression of Functional Human DNA Mismatch Repair Protein hMSH2 in Baculovirus Expression System

Hui CHEN; Zheng LI; Xiao-juan HE; Lian-xian CUI; Wei HE

2008, 28(7):  670-675. 

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Objective Expression for functional human DNA mismatch repair protein hMSH2 to use as a new candidate ligand for TCRγδ in researching of the function and antigen recognition pattern of γδT cells in immunity. Methods cDNA encoding hMSH2 was amplified with overlap-extension PCR and cloned into expression vector pAcGP67B and pET30a respectively. The recombinant vector was identified by restriction enzymes. The baculovirus DNA and pAcGP67B-hMSH2 were then cotransfected into sf9 cells. The recombinant hMSH2 protein was identified by western blotting. Meanwhile, the recombinant pET30a-hMSH2 was expressed in E.coli. The binding specificity of hMSH2 protein to OT3 peptide and TCRγδ was detected by ELISA and FCM. Functions of hMSH2 protein were further evaluated by MTT colorimetric assays and cytokines secret assay in vitro. Results The full length of hMSH2 gene was obtained by overlap-extension PCR and was cloned into the baculovirus expression vector and pET30a to construct the recombinant expression vectors, pAcGP67B-hMSH2 and pET30a-hMSH2, respectively. After 3 rounds of virus amplifying by re-infection, the soluble hMSH2 was detected in the supernatant. Purified hMSH2 not only binds specifically to OT3 peptide, but also to TCRγδ. Moreover, results indicated that the resultant protein can trigger the proliferation and IFN-γ secreting of γδT cells in vitro. Conclusion Functional hMSH2 recombinant protein was successfully expressed in baculovirus expression system holding more activity than that expressed by E.coli.

Interrelationship and Effects of Ischemic Postconditioning on Hippocampus rCBF and VEGF following Cerebral Ischemic in Tree Shrews

Hai-yun LUO;

2008, 28(7):  676-680. 

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OBJECTIVE：To approach the interrelationship between the regional cerebral blood flow （rCBF） and VEGF on CA1 of hippocampus and reveal the effect and possible mechanism of ischemic prostconditioning on rCBF and VEGF expression during focal cerebral thrombosis. METHODS: The thrombotic focal cerebral ischemia was induced by photochemical reaction in tree shrews, and ischemic postconditioning was established by clipped ipsilateral carotid of the animal at 4 hours after cerebral ischemia. The rCBF and VEGF expression in hippocampus CA1 area were detected, respectively, by laser-Doppler(LD) fowmeter and immunohistochemistry. RESULTS: The number of VEGF positive cells were increased markedly and VEGF expression enhanced after cerebral ischemic 4h,express of VEGF in 12h is the most intensification（P＜0.01）, the most attenuate in 72 h（P＞0.05）. The rCBF reduce along with temporal lasting in cerebral thrombus,especially decrease in 24h（2.55±0.28pu P＜0.01）;Then the rCBF also to a exten restoration,especially increase in 72h（18.74±1.59）.Postconditioning may gradually increase hippocampus rCBF, especially in 72h (10.42±3.75 PU ,P＜0.05) meanwhile the expression of VEGF is very high than that of ischemic except 8h （P＞0.05）,mostly in 12h（P＜0.01）; Electron microscope reveals that mitochondrium stress and endoplasmic reticulum cisterna formation of hippocampus were the most manifest in 24 h，but when we apply postconditioning on it ,the phenomenon can be relieived.CONCLUSION: The close relationship between rCBF reduction and VEGF expression in hippocampus CA1. Ischemic postconditioning may prolong “time window” of therapeutic cerebral ischemia, and the protection mechanism of ischemic postconditioning may associated with the increase of rCBF in 12h.Also ischemic postconditioning influence the rCBF of hippocampus maybe the key point of protective neuron .

Impact of endogenous hydrogen sulfide on endothelin-1 and connective tissue growth factor expressions in rats with high pulmonary blood flow

Xiao-hui LI; Jun-bao DU Ding-fang BU; Hong-fang JIN; Chao-shu TANG;

2008, 28(7):  681-685. 

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Two monoclonal antibodies reveal distinct subecellular localization of a-synuclein in rat brain neurons

Guang-wei LIU; Juan-juan YIN; Yao-hua LI; Biao CHEN; Shun YU

2008, 28(7):  686-691. 

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Cloning, Expression and Purification of Fragile X Mental Retardation Protein

Jian LIU; Ke ZOU; Ning ZHU; Yan SHEN

2008, 28(7):  692-695. 

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Abstract　Objective　Clone the human fragile X mental retardation gene 1 cDNA into the pET22b(+) vector, express and purify FMRP to study its function. Methods　The plasmid pET22b(+)-FMR1, constructed by molecular cloning, was transformed into E. coli BL21(DE3) competent cells and induced to express FMRP by IPTG. Then, FMRP was purified by affinity chromatography. Purified FMRP was tested for its RNA binding ability. Results　FMR1 cDNA was successfully cloned into pET22b(+) vector and expressed in E. coli BL21(DE3). A protein with Mr 69 000 was purified and confirmed to be FMRP by western-blot. This protein retained the RNA binding ability of FMRP. Conclusion　FMR1 gene was successfully expressed in BL21(DE3) cells, and highly purified FMRP with RNA binding ability was obtained, which provided reliable material to study the function of FMRP.

Distribution of Androgen Receptor in murine thymus and its relationship with a novel protein Rwdd1

Dai CHEN; Ning KANG; Long TANG; Qing-feng LIU; Yu HU; Lian-xian CUI; Wei HE

2008, 28(7):  696-701. 

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CCR5 promotes the migration of 6T-CEM through Human Brain Microvascular Endothelial Cells Monolayer

Yi-ran MA; De-shu SHANG; Wei-dong ZHAO; Wen-gang FANG; Yu-hua CHEN

2008, 28(7):  702-706. 

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Abstract: Objective: We have showed the stronger ability to migrate through human brain microvascular endothelial cells (HBMECs) in peripheral T lymphocytes of AD patients than age-matched is associated with a significantly higher macrophage inflammatory protein-1α (MIP-1α) expression of T lymphocyte. In order to explore the mechanism of the transendothelial migration of T lymphocytes further, we choose 6T-CEM cells expressing MIP-1α as a model cell line of AD patients’ T lymphocytes to investigate this mechanism. Methods :Immunofluorescence, western blot, plasmid construction and transfection, transendothelial migration and endothelial permeability assay are used to explore the progress of the transendothelial migration of T cells. Results: The expressions of CCR5 on HBMECs which incubated with 6T-CEM cells were detected. The induced expressions of CCR5 can enhance the migration of 6T-CEM cells through HBMEC monolayer. Conclusion: CCR5 on HBMECs might be involved in the transendothelial migration of T lymphocytes .

The expression of COX-2 in human pulmonary epithelial cells was suppressed by D-siRNAs

Hong LUO; Dong-xu HU; ping Chen

2008, 28(7):  707-712. 

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Objective Here we report the use of Dicer to cleave double-stranded RNA (dsRNA) into small interference RNAs (siRNAs) that can target multiple sites within an mRNA. Methods A549 cells were chosen to be incubated with 5ng/mL IL-1β for different time(0h,3h,6h,9h,12h,) to detect the time-dependent expression of COX-2.To generate the long dsRNA,the COX-2 gene (728bp) was amplified by PCR with a specific forward primer that contained a T7 promoter and a specific reverse primer that contained an SP6 promoter.Then,sense strand RNAs were generated by T7 RNA polymerase and antisense strand RNAs were generated by SP6 RNA polymerase.These single strand RNAs were annealed by the standard method. We mixed dsRNA with Dicer in reaction buffer. The mixture was incubated for 120 min at 37℃.We recovered siRNAs using RNA Purification Column.Transfections with diced siRNAs were performed using the TransMessenger Transfection Reagent in accordance with the manufacturer＇s instructions.COX-2 mRNA and protein were determined by RT-PCR and Western blot respectively. PGE2 was measured by ELISA. Results This study showed that IL-1β induced COX-2 protein expression in A549 cells.We recovered siRNAS that have been generated in vitro by Dicer. D-siRNAs significantly suppress the expression of COX-2 in human pulmonary epithelial .Conclusion In this study,we demonstrate that D-siRNAs significantly suppress the expression of COX-2 in human pulmonary epithelial

construction eukaryotic expression plasmid of insig2 and its influence to related genes of rat metabolism

Ke CHEN; Zhao-hui MO; Xiao-wei XING; Ping-an HU; Yan-hong XIE

2008, 28(7):  713-718. 

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Effect of water-immersion restraint stress on the nitric oxide production in rat platelets

Hao-lou FENG; Yu-ying CUI; Fang WEI; Bao-shui WANG; Chao-shu TANG

2008, 28(7):  719-721. 

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Effect of water-immersion restraint stress on the nitric oxide production in rat platelets FENG Hao-lou1，CUI Yu-ying2, WEI Fang2, WANG Bao-shui 2，Tang Chao-shu3 ( 1. Dept. Physiology, Medical College, Hebei University; 2. Dept. Physiology, College of Vocational and Technological Sanitation, Hebei University, Baoding 071000, China； 3.Dept. Institute of Cardiovascular Research, Peking University First Hospital, Beijing 100034.) Abstract: Objective To investigate the effect of water-immersion restraint stress (WRS) on the nitric oxide (NO) production and its mechanism in rat platelets. Methods Male SD rats underwent WIR stress for 2, 4 and 8 hours. Ulcer index (UI) of stomach was detected by Guth method, nitrite production was measured by Greiss assay, nitric oxide synthase (NOS) activities and L-arginine transport rate were determined by isotope tracer method. Results After 2h WRS, the NO production, the NOS activity and L-arginine transport rate in rat platelets were obviously increased, but they tended to be gradually decreased as the time went on. After 8h of WRS, these parameters were markedly decreased compared with the control group. The gastric ulcer appeared and got more serious after 4h WIR stress. Conclusion Short-term WRS up-regulated L-arginine /NO pathway and increased nitric oxide production in the platelets while long-term WIR stress caused the reversed results.

Regulation of Notch1 activity by Rab5 in pancreatic cancer BxPC3 cells

Juan ZHANG; He-fen YU; Yu-xiang ZHANG; Zhe-sheng WANG

2008, 28(7):  723-728. 

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Objective　To investigate the effect of Notch1 and Rab5 on the growth of pancreatic cancer BxPC3 cells and the regulation of Notch1 activity by Rab5 in BxPC3 cells. To investigate the effect of Wortmannin and LY294002 on the growth of pancreatic cancer BxPC3 cells and the regulation of Notch1 activity. Methods　Notch1 and Rab5 expression were inhibited by siRNA interference. The growth rate of BxPC3 cells was measured by MTT. Rab5 expression was inhibited by siRNA interference ,the changes of Rab5 and Notch1 protein were measured by Western blot . Results　The growth rate of BxPC3 cells decreased after inhibiting Notch1 and Rab5 expression. Notch1 protein level decreased after inhibiting Rab5 expression. After adding Wortmannin and LY294002, the growth of BxPC3 cells and Notch1 protein level decreased . Conclusion 　In BxPC3 cells, Notch1 and Rab5 are important to cell growth. Rab5 may regulate Notch1 activity. Wortmannin and LY294002 are important to cell growth and regulate Notch1 activity.

Association of gene-gene interaction of TNF-a and VDR loci with hepatitis B virus chronic infection in Han population of north china

Ji-rong GAO; Hong-quan LI; Zhuo LI; Ying LIU; Jun-hong LI; Xian-jia ZENG; Hui LI

2008, 28(7):  729-733. 

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Effect of - 1 SNP on CCNH promoter activity

Pei-lin CHEN

2008, 28(7):  734-737. 

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Objective 　To study the effect of -1 site single nucleotide polymorphism ( SNP) on CCNH gene promoter transcription activity. Methods　PCR and site-directed mutagenesis technology were used to construct CCNH basic promoter and -1G mutate promoter. Dual-Luciferase Reporter assay system was used to detect the transcription activity of constructed promoter. Results 　In AD293 cells, the activity of - 1G mutate type promoter was significantly lower than wild type -1T promoter（P<0.05）. Conclusion　It is implied that SNP-1T→G base variation can decrease the transcription activity of CCNH gene promoter.

Expressions and significances of cyclinD1 CDK4 and PCNA in pancreatic carcinoma

Yong YANG; Yun-ning HUANG; Gang SU; Shi-jie YANG; Bao-guo YAO

2008, 28(7):  738-741. 

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【Abstract】 Object To investigate the expressions of cyclinD1 CDK4 in pancreatic cancer and their relationships with clinlicopathological parameters. Methods Immunohistochemical detector with new second generational tow steps method was used to detect the expressions of cyclinD1 CDK4 and PCNA in 48 cases of pancreatic cancer and 14 cases of chronic pancreatitis. Results The expressions of cyclinD1 CDK4 proteins in pancreatic cancer were 70.85%（34/48）and 62.50%（30/48） respectively .The cyclinD1 and CDK4 proteins were increased（P<0.01）, as compared with that in chronic pancreatitis. The positive rates of cyclinD1 and CDK4 in the pancreatic cancer of well-differentiation, without metastasis and clinical stage Ⅰ/Ⅱwas significantly lower than that in the cases of poor differentiation ,with metastasis and clinical stage Ⅲ/Ⅳ（P<0.05）.The PCNA counting score in cyclinD1 positive group was higher than those in cyclinD1 negative group（P<0.01）; The PCNA counting score in cyclinD1 positive group was higher than those in cyclinD1 negative group（P<0.05）.Concluslons: cyclinD1 and CDK4 are attend to the carcinogenesis and progression of pancreatic cancer, and is related to the activity of cell proliferation . Examination of CyclinD1 and CDK4 may be as an marker to judge biological behavior of pancreatic cancer.

RBP4 levels and-G 803 A SNP in relation to metabolism syndrome

Ling-ling XU; Xin-hua XIAO; Qi SUN; Heng WANG

2008, 28(7):  742-746. 

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Changes of Substantia Nigral Dopaminergic Neurons and TNF-α in Serum Induced by LPS

Li ZHANG; Yan-yong WANG; Dong-sheng CUI; Ming-wei WANG

2008, 28(7):  747-751. 

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Objective To study the degeneration of dopaminergic neurons and the relationship between it and the level of TNF-α in serum by intranigral injection of LPS. Methods LPS or PBS was stereotaxically injected into substantia nigra(SN).At different survival times,the damage to the substantia nigral DA neurons was observed by using tyrosine-hydroxylase immunohistochemical staining and the level of TNF-α in serum was measured by Radioimmunoassay. Results 24 hours after LPS injection,there was apparent reduction in TH-positive cells on the LPS-injected side.The decrease reached a peak after 14 days.In the subsequent days,the degree of degeneration remained at the stage. Differences were observable when compared the level of TNF-α in serum of 24h、14d、28d with that of the PBS group.The former was higher. The study revealed linear correlation between the level of TNF-α and the survival rate (percentage of right TH-positive neurons to left). Conclusion The degeneration of TH-positive neurons induced by inflammation was in time-dependent manner. While in development of PD the effect of inflammatory damage was only early stage；In PD local inflammation in substantia nigra may be induce systemic inflammatory reaction; The level of TNF-α in serum is related to the degeneration of TH-positive neurons in substantia nigra.

Three Fluoroquinolone Prolonged Cardiac Action Potential Duration

Shu-yu SHANG;

2008, 28(7):  752-755. 

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Abstract Objective To compare cardiaotoxic effect with sparfloxacin and levofloxacin, the effects of antofloxacin on cardiac action potentials were examined. Methods Action potentials were recorded from isolated papillary muscles of guinea pig right ventricles by using conventional microelectrode techniques. Results 1.With stimulation basic cycle length of 1000ms, both APD50 and APD90 were prolonged at any used concentrations by sparfloxacin, While, at the concentrations over 10μmol/L, levofloxacin and antofloxacin prolonged the APD50 and APD90. 2. A the concentrations used by 10μmol/L, antofloxacin prolonged the APD90 were decreased by E-4031, and were increased by Chromanol 293B, no obviously changed by E-4031 and Chromanol 293B.3.With the stimulation basic cycle length increased, the prolongation of APD90 caused by those three drugs were increased，but no frequency dependency. Conclusion: Antofloxacin has less potency to prolong APD as compare to LVFX and SPX, and it maybe prolong APD by inhabit IKr.

Effections of TIMP-3 Gene Transient Expression on Proliferation,Migration and Apoptosis of Rabbit Vascular Smooth Muscle Cells

Jin-jie CHEN; Xiao-dong PU

2008, 28(7):  756-759. 

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Objective Observe effections of TIMP-3 on proliferation, migration and apoptosis of rabbit vascular smooth muscle cells（VSMC）transfected by recombinant plasmid vectors mediated by cationic liposome and then discuss the TIMP-3 gene therapy on in-stent restenosis（ISR）. Methods Divide VSMC into three groups as normal control group, empty plasmid group and recombinant group,and then do transfection separately.First, at time of 24hours, 48hours and 72hours , calculate cells ,quantify MMP-2 and analyse TIMP-3 mRNA. Second, measure cell apoptosis rate after 48hours. Third, establish a migration model ,then calculate migrated cells after 48hours. Resuls There were no difference between normal control group and empty plasmid group in cells grow, MMP-2’s content , TIMP-3 mRNA expression and apoptosis rate,cells migration rate.While recombinant group had obvious difference compared with the other two,P<0.05. Conclusions It is testified TIMP-3 can strongly inhibit proliferation or migration of VSMC in vitro, but promote apoptosis on the other hand. Cationic liposome is a safe medium which can provide broad application in TIMP-3 gene therapy on ISR.

技术与方法

The islet x cells rat models were established by the islet B cell-deleting teconology

Lin PAN; Rui-qin DU; Hong-liang LI; Wen-ying YANG; Guang-wei LI

2008, 28(7):  760-764. 

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In vivo Quantitative Measurement of Glutamate & Glutamine in Zelanian Rabbits’ Muscle using MRS ——Research on Feasibility and Accuracy

Fan ZHANG; Yue-ping PAN; Gui-zhen HE; Ling HU; Xiao-zhen LI; Zheng-yu JIN

2008, 28(7):  765-769. 

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Objective: Try to Use MRS technology to detect Glutamate & Glutamine’s total concentration in Zelanian rabbits’ in vivo muscle. Via biopsy, prove the accuracy of this method. Methods: Select 20 healthy Zelanian rabbits, using SS-PRESS sequence to collect their Signal Ratio of Glx/TCr. After the collection, let blood and do the biopsy of the observed area of the collected muscle tissue immediately. Mensurate the rabbits’ blood Crn concentration, Glx and TCr concentration in muscle. Using SPSS software to research the correlation between the Glx/TCr Signal Ratio and Glx/TCr concentration in muscle, attempt using the MRS Signal Ratio and blood Crn concentration to predict Glx concentration in muscle. Results: The correlation between Glx/TCr Peak Ratio and Glx/Crn ratio is 0.681. We performed a linear regression and got the formula: The predict Value of Glx concentration in muscle （umol/g muscle）= Glx/TCr Peak Ratio × Crn concentration in blood（mg/dl）× 28.754－0.631. Conclusion: Using the MRS method, the detected concentrations of the substances had a strong positive correlation with the biopsy values. Using a linear regression formula to predict the Glx concentration, the results can reflect the level of the true values, though it’s not accurate enough for quantitatively analysis, the new method can avoid biopsy.

研究短文

Effects of AG to the expression of α-SMA on pulmonary fibroblasts from pulmonary fibrosis model rats

Yu-min SUN; Li-ping ZHANG; Fu-cheng SONG; Jing DONG

2008, 28(7):  770-771. 

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Abstract: Objective To observe the effects of aminoguanidine(AG) on transforming growth factor -beta1(TGF-β1) induced expression of -smooth muscle actin( -SMA) of the fibroblasts in lung from pulmonary fibrosis model rats in vitro. Methods The pulmonary fibroblasts from pulmonary fibrosis model rats were isolated from explants . We selected the proper concentrations of TGF-β1 and AG by MTT. The positive cells -SMA were observed by immunocytochemical staining. The expression of -SMA was examined by flow cytometry. Results The expression of -SMA of fibroblasts in TGF-β1 group were higher than those of Sham group ( P<0.01). Compared with TGF-β1 group, the expression of -SMA in AG+TGF-β1 group were lower (P<0.05); but compared with Sham group, they were higher significantly(P<0.01).There were no significant difference between AG group and Sham group（P>0.05）.Conclusion TGF-β1 induced the expression of -SMA in pulmonary fibroblasts from rats on d14 after administration of BLMA5 and accelerated the differentiation of fibroblasts to myofibroblasts. However, AG decreased these roles of TGF-β1.

The Experimental Study of Radiofrequency Catheter Ablating at Coronary Sinus Ostium

Li-yin HAN; Shuan-li XIN; Fan LIU; Zhi-mei LI; Xiao-min BAI

2008, 28(7):  772-773. 

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Objective To study the histopathological change in the radiofrequency cather ablation(RFCA) region adjacent to Coronary Sinus Ostium (CSO) and the correlation between the amount of energy and the size of jeopardized lesions after ablation. Methods Twenty Yorkshire pigs were randomized into control group and experimental group. Pigs of experimental group were ablated with 30W for different time and the control group was euthanasized as blank control.Observe the histopathol- oical change in the ablated region with light microscope, statistically analyzed the correlation between the amount of RFCA energy and the size of lesions. Results The ablation targes were localised lesions, there was no significant difference in area between 10S and 20S group when ablated with power of 30W(p>0.05). The injured area was significantly increased when the ablation time extended to 30s and more(p<0.05). The depth of RFCA lesions would be significantly deeper than that of 10s when the time extended to 20s and more(p<0.05), while the depth of RFCA lesions had no linear correlation with the amount of energy. Conclusions The injured size near CSO was significantly increased when the discharging time extended to 10S or 20S and the lesion area would correspendingly increase with the energy which ranged from 300J to 2400J.

临床园地

The Sedation of Propofol Combined with Midazolam in Patients undergoing Long-time Microsurgical Operation

Gao-feng ZHANG; Gang LI; Yong-guang XU

2008, 28(7):  774-777. 

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Objective：To investigate the sedation effect of propofol combined with different dose of midazolam in patients undergoing long-time microsurgery taken auditory evoked potential index (AAI) as an end point. Methods：Forty ASA I～II patients were randomly divided into 4 groups. Normal saline(NS) 2ml for group Ⅰ, midazolam 0.01mg/kg, 0.02mg/kg , 0.04mg/kg for GroupⅡ, Ⅲ, Ⅳ separately. 1 min after given NS or midazolam, propofol was infused at a rate of 300～500mg/h combined with midazolam at a rate of 0.01mg/(kg•h), 0.02mg/(kg•h), 0.04mg/(kg•h) for GroupⅡ, Ⅲ, Ⅳ separately. After the AAI reduced to 40(sedation induction), adjusted the infusion rate of propofol to keep AAI at the level of 30～45, and sustained the level by infusing propofol and midazolam for 5 hours. The dosage of propofol required for sedation induction and sedation sustaining were recorded. 5 hours later, stopped the infusion, and recorded the analepsia time and whether patients had awareness during the operation. Results：According to the sedation target level of AAI30～45, the OAA/S score of all patients in the four groups could reach 0～1.The induction period was obviously shortened and the propofol dosage was dramatically reduced in Group Ⅱ～Ⅳ compared with GroupⅠ. In the stage of sustaining the sedation , the dosage of Propofol in both GroupⅠandⅡ were used more than that in Group Ⅲ and Ⅳ, however there were no significant difference between Group Ⅲ and Ⅳ. The analepsia time in Group Ⅳ was dramatically longer than in the other groups. Conclusion：The patients could got a satisfactory sedation by propofol or combined with midazolam during long-time microsurgical operation .The optimal compatibility program was that midazloam was administered 0.02mg/kg for sedation induction, and propofol was infused combined with midazolam 0.02mg/(kg•h) when sedation sustaining.

短篇综述

Research advancement of leptin and prostate cancer

Jie-mei YE

2008, 28(7):  778-780. 

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Abstract：Leptin, a pleiotrophic hormone mainly synthesized by adipocytes, is an important signaling molecule in energy regulation and food intake. Recent studies have shown that Certain cancers are associated with leptin.This article reviews leptin’s pathophysiological role in prostate cancer progression.

Spermatozoa proteomics study progress

Yong-bo PENG; Zong-yin QIN; Yong-peng XIA; Yong-ping MA

2008, 28(7):  781-784. 

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Proteomic technology widely used to research differently expressed proteins under different physiology and pathologic state. In the aspect of the human spermatozoa proteomics, many scholar have researched the differently expressed proteins under physiology and pathologic state by modern proteomics technology, revealed the change rule of sperm under different physiology and pathologic state from proteome level, and provided reliable theoretic base for deeply understand sperm molecule mechanism of physiology and pathologic state.