Basic & Clinical Medicine

Recommendations on strengthening the development of nuclear medicine in China

Shih-chen WANG Zheng-fu LU

2009, 29(10): 1009-1016.

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This paper outlines briefly the role of nuclear medicine in life sciences and health care. Molecular imaging by using isotopic tracers can noninvasively visualize the chemistry or hidden process in the cells and tissues inside the body, obtaining "functional" images to provide early information of any disease and revealing the secrets of life. The vitality of nuclear medicine is its ability to translate bench into new clinical application that can benefit the patients. Although nuclear medicine community in China has made significant achievement with a great effort since 1950s, there are many obstacles to future development. Recommended measures are proposed here in an attempt to solve our existing problems.

Inhibition of entecavir on hepatitis B virus (HBV) replication in HBV replication mice

Feng-jun LIU; Li LIU; Zhi JIANG; Cong LIU; Hong TANG

2009, 29(10): 1017-1020.

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Objective To investigate whether the HBV replication mouse model can be used for selection of anti-HBV drugs. Methods HBV replication-competent plasmid pHBV4.1 was transferred into male BALB/C mice by using hydrodynamics-based in vivo transfection. The mice were matched by body weigh, age and serum levels of Hepatitis B e antigen(HBeAg) and were devided into experiment group and control group (4 mice in each group) 24 h after transfection and were treated orally with entecavir and normal saline for three times at intervals of twenty four hours, respectively. HBV mRNA and HBV DNA replication intermediates in liver were analyzed by Northern and Southern Blot Hybridization, respectively. The serum HBsAg and HBeAg were detected by enzyme linked immunosorbentassay (ELISA). Results The results of two indepent experiments showed that compared with the control animals, HBV DNA replicative intermediates dramatically decreased in the liver from the experiment mice. The levels of HBV mRNA from liver and the serum HBsAg and HBeAg , however, were not obviously different. Conclusions The inhibition effect of entecavir on HBV was detected in the HBV replication mice, suggesting that the HBV replication mouse model can be used for selection of anti-HBV drugs.

Sulforaphane protects motor neurons of rat spinal cord via inducing phase Ⅱ enzymes

Ji-xu YU; Yan-su GUO; Geng CHANG; Ya-qiong JIA; Chun-yan LI

2009, 29(10): 1021-1025.

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Objective To investigate whether sulforaphane (SF) protects motor neurons via inducing phase Ⅱ enzymes. Methods The SD rat pups spinal cord organotypic cultures were divided into three groups at random: control, 100?mol/L THA group, 10?mol/L SF plus 100?mol/L THA group. The number of motor neurons was assessed by immunohistochemistry, and the expression of phase Ⅱ enzymes were assayed with western blot. Results At the end of 3 weeks, the motor neurons number in the group treated with THA was less than the control group (P<0.05, n=10-15). However, the motor neurons number in the group treated with SF plus THA was more than group treated with THA at the end of 3 weeks (P<0.05, n=10-15). Meanwhile, the phase Ⅱ enzymes (NQO-1 and HO-1) in the group treated with SF plus THA expressed more than group treated with THA only (P<0.05, n=3). Conclusions SF could prevent motor neuron death caused by THA toxicity via inducing the expression of phase Ⅱ enzymes.

Effect of MMP-28 antisense oligodeoxynucleotide on cell biological behaviour of human lung cancer cell

Yang ZHANG; Yong LIN; Xiao-jun WANG; Jirigala; Hashengaowa

2009, 29(10): 1026-1030.

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Objective To study the effect of matrix metalloproteinase (MMP)-28 antisense oligodeoxynucleotide (AODN) on cell biological behaviour of human lung cancer cell A549. Methods To explore its possible functions, cell line A549 was subjected to specific inhibition of MMP-28 by AODN transfection. RT-PCR and Western blot were used to evaluate mRNA and protein level of MMP-28. The proliferative status of cells was measured by MTT assay and soft-agar colony formation assay.Cell invasion ability was detected by Matrigel in vitro invasionassay. The ability of tumor formation and metastatic potency was examined by lung cancer cells xenograft in nude mice. Results After 48 h transfection, the expression of MMP-28 mRNA and protein in AODN-transfected cells was significantly lower than those of control cells and SODN-transfected cells (P<0.01). The ability of proliferation and invasion of AODN-transfected cells was decreased (P<0.01).The weight of xenograft in AODN-transfected cells wasfar less than those of in SODN-transfected cells(P<0.01). Conclusion Both cell proliferation and invasion potential of A549 cells in vitro and in vivo were inhibited effectively by MMP-28 AODN, suggesting that MMP-28 may be a promising molecular target for anti-invasion therapy of human lung cancer.

Alteration of NO、ET-1、MMP-9、TIMP1 plasma levels in coronary artery stenosis and ectasia

Lian-feng CHEN; Yi YAN; Yi-an YAO; Shu-yang ZHANG

2009, 29(10): 1031-1034.

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Objective To explore the mechanisms responsible for different coronary artery lesions involved in nitric oxide (NO), endothelin -1 (ET-1), matrix metalloproteinases -9 (MMP-9) and the matrix metalloproteinase inhibitor -1 (TIMP -1). Methods The cases undergone coronary anography in our hospital were collected and divided into three groups: group A,30 patients with the coronary artery ectasia(7 cases of simple coronary artery ectasia; 18 cases of the coronary artery ectasia coexisting a small amount of plaque)；group B,38 patients with coronary atherosclerosis; group C,32 patients with with normal angiograph (14 cases of coronary artery completely normal; 18 cases with a small amount of coronary plaque only). Plasma NO, ET-1, MMP-9 and TIMP-1 level were measured by ELISA method. Results There are significant differences among three groups on NO level, MMP-9 levels, NO/ET-1 and MMP-9/TIMP-1 (P< 0.05). Further sub-group analysis, NO advanced to peak value in isolated coronary ectasia, while MMP-9 rose to highest value in the coronary artery ectasia coexisting a small amount of plaque. Conclusions The coronary ectasia may be the normal compensatory function to resist the coronary artery atherosclerosis ,while coronary atherosclerosis is the lower of compensatory function.

The packaging and purification of recombinant adeno-associated virus loaded with anti-amyloid β peptide single-chain antibody gene

Jiong CAI; Yan-wei ZHONG; Fang LI; Shi-zhen WANG

2009, 29(10): 1035-1038.

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Objective To produce and purify recombinant adeno-associated virus (AAV) loaded with anti-amyloid β peptide single-chain antibody gene for Alzheimer's disease gene therapy. Methods The plasmid pSNAV2.0-Abeta-scFv was utilized to transform BHK-21 cell by Lipofectamine 2000 for stable package cell line establishment. The helper virus HSV1-rc/ UL2 was used to transfect package cell for anti-amyloid β peptide single-chain antibody gene loaded recombinant adeno-associated virus production. The chloroform-PEG/NaCl-chloroform extraction and ion-exchange chromatography were employed for recombinant AAV purification. SDS-PAGE and PCR amplification were adopted for purified virus identification. The final virus physical titer was determined by digoxin-labeled DNA probes. The effect of gene therapy was tested with transgenic mice by Water maze test. Results The purity of recombinant adeno-associated virus with our target gene reach up to 98% after stable cell package and serial purification. The physical titer of the final virus was 1×1012vg/ml. The latency of treated mice in water maze test were reduced significantly. Conclusion The adeno-associated virus carrying anti-amyloidβ peptide single-chain antibody gene was produced by HSV1system and purified. The animal behavior test demonstrated the recombinant AAV was effective in Alzheimer's disease gene therapy.

Expression and purification of HPV 18 L1 virus like particles and preparation of guinea pig antiserum against HPV 18 L1 VLP

Qing-lin PENG; Ting ZHANG; Dong-sheng FAN; Wei ZHENG; Xi-xiu XIE; Yu-fei XU; Xue-mei XU

2009, 29(10): 1039-1043.

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Objective To obtain human papillomavirus type 18 virus-like particles using an optimized HPV 18 L1 gene and prepare antiserum from guinea pig. Methods HPV 18 L1 gene was optimized by combined strategy including codon replacement, truncation of 32 amino acids at C terminal and reduction of secondary structure of mRNA. The optimized HPV 18 L1 gene was synthesized and cloned into pFastBac1 vector. The recombinant baculovirus was obtained and then used to infect sf9 cells. 72h later, the cell lysate was analyzed by western blot. HPV 18 L1 VLP was purified by gradient centrifugation and analyzed by SDS-PAGE and transmission electron microscopy. The guinea pig was immunized with purified HPV 18 L1 VLPs, and the Freund's adjuvant was used. Then antiserum against HPV 18 L1 VLP was produced. Results Optimized HPV 18 L1 gene was expressed efficiently in sf9 cells. The HPV 18 L1 VLP could be purified by gradient centrifugation and the purity was over 90%. Electron microscopy showed the VLP was about 50nm in diameter. The serum antibody titer of guinea pig was 5.12×105. Conclusions HPV 18 L1 VLP could be expressed efficiently in sf9 cells using optimized HPV 18 L1 gene. And guinea pig antisera could be used for further research of HPV 18 L1 VLP based vaccine.

shRNA expression targeting gene FAK and the effects on biological behavior of hepatic stellate cells

Jun-yan AN; Xiao-lan ZHANG; Dong-mei YAO; Zhi-na DUN; Shu-rui XIE; Li-seng HAO

2009, 29(10): 1044-1049.

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Objective To investigate the role of small hairpin RNA (shRNA) targeting FAK gene on activation and proliferation in rat hepatic stellate cells （HSC）. Methods Two pairs of DNA sequences containing small hairpin structure and one pair of non-specific control sequence were cloned into the plasmid pGenesil-1 and the recombinant plasmids were then transfected into HSC-T6 by cationic polymer gene transfection reagent. The expression of FAK was detected by real-time PCR and Western blot. The proliferation of HSC was detected by MTT assay. The expression of the marker of HSC activation, α-smooth muscle actin (α-SMA), was assessed by Western blot. Results The FAK shRNAs could inhibit the expression of FAK at mRNA and protein levels, and shRNA2 could decrease FAK expression by 76.82% in mRNA level. FAK shRNAs could significantly inhibit the proliferation of HSC and decrease the protein expression of α-SMA in rat HSC. Conclusion The specific FAK shRNA may have an inhibitory effect on the proliferation and activation in rat HSC.

The effects of bone marrow mesenchymal stem cells administration on expression of VEGF、SDF-1、TGF-β1 and heart function in myocardial infarcts rats

Yi-qing WANG; Yuan-peng LI; Bing-bo HOU; Jin-cun GUO; Peng ZHANG

2009, 29(10): 1050-1053.

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Objective To investigate the effects of bone marrow mesenchymal stem cells administration on expression of VEGF、SDF-1、TGF-β1 and heart function in myocardial infarcts rats. Methods MSCs were harvested from SD rats, collected from bone marrow, cultured and passaged in vitro, then purified by density gradient centrifugation and adhesive-screening method. Rats were randomly divided into 2 groups. In the control group (n =8) were not treated with stem cells as the experiment group, but saline of the same volume. Four weeks after the injection,the expression of VEGF、SDF-1、TGF-β1 and heart function were examined. Results Four weeks after the transplantation，Immunohistochemistry showed expression of VEGF and SDF-1 increased markedly in experiment group,and expression of TGF-β1 decreased. Heart function was improved in the experiment group(P<0.05). Conclusion MSCs transplantation can interfere in the secretion of VEGF、SDF-1、TGF-β1 and improve the heart function.

TrKA -siRNA inhibits the expression of NF-κB and promote the apoptosis of breast cancer

Ju ZHANG; Chang-jie CHEN; Chen-biao LIU; Qing-ling YANG; Feng-meng TENG

2009, 29(10): 1059-1064.

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Objective Investigate the effect of the change of TrKA gene on the expression of neucleoprotein NF-κBp65 and apoptosis. Method To construct the expression vector of TrKA small interfering RNA,The recombinant was transfected into MCF-7 cells. the stable cell line expressing TrKA small interfering RNA were selected by G418. The mRNA and protein of TrKA were tested by real-time PCR,Western-blot and Immunohistochemistry.The change of neucleoprotein NF-κBp65 was detected by WB, Flow cytometry was used to observe the cell apoptosis. Result The expression vector of TrKA-siRNA was successfully constructed.The mRNA and protein of TrKA was decreased by 74.7% and 80. 5% respectively ( P < 0. 01).The positive control of GAPDH was decreased by 85.0% (p<0.05). The change of neucleoprotein NF-κBp65 was dramatical after treat the MCF-7 cells with NGF for 2 hours.but not to the siRNA cells. Compared with MCF-7 group,the apoptosis of the group of TrKA-siRNA increase obviously(p<0.05),but NGF can not enchance the effect. Conclusion Interfering the expression of TrKA gene effectively,could inhibit the activity of neucleoprotein NF-κBp65,increase the apoptosis of breast cancer cell MCF-7 independent of NGF.

Effects of fluctuant high blood glucose on expression of apoptosis-related genes in hippocampus of diabetic rats

Wei LI; Juan SUN; Song-lou YIN; Xiu-qin LI

2009, 29(10): 1065-1069.

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Objective To investigate the effect of fluctuant high blood glucose on observing the changes of pathology and BCL-2 ,BAX expression in neurons of the hippocampus from diabetic rats. Methods The diabetic rat models were produced by injection with l％ streptozotocin(STZ),followed by insulin administration . regular insulin were used to set up fluctuant high glucose group model. After 12 experimental weeks, the express of Fas protein in hippocampus was studied by immunohistochemistry , changes of pathology and ultrastructure were observed by using light microscopes and electron microscopes in three groups .Results：Compared with those in N group，the expression of BCL-2 in pyramidal cells of diabetic rat's hippocampus was lower，while that of BAX was higher (P<0.01)．The pathologic changes of retrogression were significantly serious．The pathologic changes in RDM group were more serious in fluctuant high blood glucose rats group (P<0.05) Conclusion Fluctuant high blood glucose induces more apoptosis in hippocampal neurons cells .The expression of BCL-2, BAX may be one of the mechanism that hippocampus neuron injury in diabetic rats was aggravated.

Role of adipokines on hepatocellular apoptosis in mice

Hong-zhu YIN; Cai-yan ZHAO; Ying-hui LIU; Yan-gai SONG

2009, 29(10): 1070-1074.

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Objective To investigate the interference role that adipokines----adiponectin and leptin exert to hepatocellular apoptosis in mice suffering acute liver injury induced by Concanavalin A (ConA). Methods In the present study, 26 male BALB/c mice were divided to four groups randomly: ConA group( 8 mice): ConA were intravenous (i.v.) injected; adiponectin group(5 mice): adiponectin were intraperitoneal (i.p.) injected before ConA i.v. injected; leptin group(5 mice): leptin were i.p. injected before ConA i.v. injected; control group(8 mice): normal saline was i.v. injected. Mice were sacrificed 8 hours after ConA-treatment for observing hepatic pathology and hepatocellular apoptotic rate. Results Comparison to ConA group ,the mice in adiponectin group developed reduced hepatic injury and lower ALT level and hepatocellular apoptotic rate（p<0.01）, but those in leptin group developed heightened hepatocellular apoptotic rate（p<0.01）and similar ALT levels. Conclusions Adiponectin might inhibit hepatocellular apoptosis and control hepatic inflammation, but leptin improved hepatocellular apoptosis and had no effection on ALT levels induced by ConA .

Correlation between cold hemoagglutinin and diffuse panbronchiolitis in Chinese patients

Lan WANG; Bai-qiang CAI

2009, 29(10): 1075-1078.

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Objective To anaylaze the correlation between cold hemoagglutinin(CHA) and diffuse panbronchiolitis(DPB) in Chinese patients. Methods 18 patients diagnosed as DPB from December 1996 to July 2008 in Peking Union Medical College and 60 cases of DPB reported in mainland China from 1996 to 2008 were enrolled in the study. Result Of 18 patients diagnosed DPB in Peking Union Medical College, Only 1 patient had titer of CHA≥1:64. Of 60 cases in mainland China, 48 cases had CHA result. CHA was positive in 54.1% all cases.There may be some correlation between positive-Frequency of CHA and treatment、population et al. Conclusions Low positive-frequency of CHA in Chinese subjects, which is different from Japanese DPB patients, suggests that CHA may not be applicable to the clinical diagnostic criteria for Chinese patients.

Expression and significance of CD26/DPPⅣand galectin-3 in papillary thyroid carcinoma

Nian-chun PENG; Xiao-hui GUO; Zhen-fang YUAN; Yan-ming GAO

2009, 29(10): 1079-1082.

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Objective To explore the expression and significance of CD26/DPPⅣand galectin-3 in papillary thyroid carcinoma. Methods Expression of CD26/DPPⅣ or galectin-3 in 68 papillary thyroid carcinomas and 36 thyroid adenomas were determined with EnVision immunohistochemical technique respectively. Results CD26/DPPⅣ and galectin-3 were higher expressed in most of papillary carcinomas but absent or lower expressed in most of all thyroid adenomas. With CD26/DPPⅣ as means to detect papillary carcinoma, the sensitivity, specificity, diagnostic accuracy were 86.8%, 97.2% and 90.4%, and with galectin-3 the respective figures were 97.1%, 91.7% and 95.2%. There were not any statistic significance within the intrathyroidal and extrathyroidal invasion, without and with lymph node metastasis, low and high dangerous prognosis groups about positive expression of CD26/DPPⅣ or galectin-3 in papillary carcinomas. Conclusions Both CD26/DPPⅣ and galectin-3 are potential markers of papillary thyroid carcinoma. CD26/DPPⅣ or galectin-3 immunohistochemical staining can be used as a supplement for conventional pathological diagnostic approach to differentiate thyroid papillary carcinomas from adenomas, while their uses to assess the invasion , metastasis and prognosis of papillary thyroid carcinomas are out of value.

Apoptosis and mitochondrial membrane potential change of gastric smooth muscle cells in diabetic rats

Hong-xian ZHAO; Xia CHEN

2009, 29(10): 1083-1086.

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Objective To investigate the associated mechanism of gastroparesis in Diabetic Rats. Methods SD rats were randomly divided into control group and diabetic group. 10 weeks later, gastroparesis model was detected. The apoptosis rate and mitochondrial membrane potential of the gastric smooth muscle cells were detected by flow cytometry.Cytochrome C was detected by immunohistochemistry. Results Compared with rats in control group: ①Typical symptoms of diabetes mellitus appeared; The rate of gastric residual pigment was significantly higher（P＜0.01）. ②Apoptosis rate was significantly higher（P＜0.01）; Mitochondrial membrane potential was significantly lower（P＜0.01）; Cytochrome C was significantly higher（P＜0.05）. Conclusions Mitochondrion contributes to smooth muscle cell apoptosis ,which paly a partial role in diabetic gastroparesis.

Calcineurin-NFAT pathway mediates phenylephrine-induced vascular smooth muscle cell proliferation

Xiao PANG; Pei-ying HE; Ning-ling SUN

2009, 29(10): 1087-1091.

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Objective To determine whether calcineurin-NFAT pathway is involved in the regulation of VSMCs proliferation induced by catecholamines. Methods Primary VSMCs from rat aorta were used as the experimental model. Proliferation of VSMCs were measured by MTT assay and cell count. Calcineurin protein and its activity were assayed using immunoblotting and free inorganic phosphate content analysis respectively. Localization of NFATc1 was detected by immunofluorescence staining. Results Phenylephrine (PE, an α1-adrenoceptor agonist) increased VSMCs proliferation. Prazosin (an α1-adrenoceptor antagonist), cyclosporin A (CsA, an inhibitor of calcineurin) and chelerythrine (an inhibitor of PKC) decreased PE-induced absorbance and cell number. Timolol (β-adrenoceptor antagonist) has no effect on absorbance and cell number induced by PE. Additional treatment with CsA further inhibited PE-induced absorbance and cell number compared with the chelerythrine pretreatment group. CsA and chelerythrine alone had no significant effect on either absorbance or cell number. CsA decresed PE-induced calcineurin level and its activity. NFATc1 was translocated from cytoplasm to nucleus upon treatment with PE. This translocation was reversed by CsA. Conclusions CsA partially inhibits PE-induced VSMCs proliferation via inhibiting calcineurin activity and NFATc1 nuclear translocation. Calcineurin-NFATc1 pathway is involved in hyperplastic growth of VSMCs induced by catecholamines.

Effect of thyroid hormone on the electrophysiological properties and Cx43 of canine atria

Chong-han GAO; Ling-kun SONG; Fan LI; Yin DAI; Yue-hui YIN

2009, 29(10): 1092-1096.

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Objective To determine whether atrial tissue shows alterations in electrophysiological proterties and in the expression and distribution of Cx43 in dogs with hyperthyroidism induced by Levothyroxine. Methods Sixteen mongrel canines of either sex weighing 12-17kg were randomized into control group (n=6) and L-thy group (n=10). Levothyroxin (80μg/kg) was administered daily by intraperitoneal injection for 8 months to all dogs in the L-thy group. Every 2 months atrial effective refractory period (AERP) was measured in both groups. Atrial tissues were collected at the 8th month. The expression of Cx43 in atria was evaluated with western blot analysis. Cx43 in atria was located by immunofluorescence and detected with Laser Scanning Confocal Microscope. Results compared with the control group, AERP of the L-thy group in the 4th, 6th and 8th month was significantly shorter (P<0.05). Levothyroxine significantly reduced the expression of Cx43 in left and right atrial (P<0.01), and the heterogeneous Cx43 down-regulation between left and right atria in the L-thy group was significant (P<0.05). Connexin43 located at the intercalated disc of atrial myocardium in the L-thy group was significantly reduced (P<0.01). Conclusions Thyroid hormone leads to atrial electrical remodeling and gap junction remodeling, involving heterogeneous reduction in Cx43 expression and disturbance in the distribution of Cx43. These changes are components of a variety of remodeling processes necessary, although not causative, for AF to become sustained.

Preparation and identification of anti-human BPI23 monoclonal antibody

Zhi-ying HU; Yun-xia WAN; Su-juan GUO; Yun LI; Xiu-juan HE; Jun LONG; Yun-qing AN

2009, 29(10): 1097-1101.

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Objective　Preparation of anti-human BPI23 monoclonal antibody with hybridoma technique and preliminary analysis of the application. Methods The immunized mouse spleen cells and mouse myeloma cells were fused by routine method;the methods of indirect ELISA and Western-blot were used to screen the hybridoma cells which secreted monoclonal antibody;the positive clones were subcloned for three times with limited dilution method to acquire hybridoma cells which secreted anti-human BPI23 monoclonal antibody stably and efficiently;the amplified hybridoma cells were injected into abdominal cavity of mouse and the ascites was collected;the antibodies in ascites were purified,the type,class and subclass of antibodies were detected;the specificity of the antibody was analyzed by Western-blot;the titer of the monoclonal antibody was detected by indirect ELISA;the normal human neutrophil and mononuclear cells were separated and purified and were used for smear making,anti-human BPI23 monoclonal antibody was used to detect by immunohistochemistry. Results　Three hybridoma cell lines(1B4、9C12 and 2H11)secreting anti-human BPI23 monoclonal antibody stably and efficiently were acquired,The anti-human BPI23 monoclonal antibodies secreting by hybridoma cells belonged to kappa opioid IgM class,kappa opioid IgG1 subclass and kappa opioid IgG1 subclass respectively;the titer was 1.28×105、1.28×105 and 4.1×106 respectively,the content of purified antibodies was 0.208g/L、2.03g/L and 3.88g/L respectively;Western-blot showed that the monoclonal antibodies in ascites of tumor-bearing mice can recognize human BPI23 protein prepared in our lab and human BPI55 standards saled in the market specifically as well,but can't bind mouse BPI25 and human LBP;in immunohistochemistry, the 1B4、9C12 and 2H11 monoclonal antibodies can recognize BPI of human neutrophil specifically. Conclusion　The monoclonal antibodies against human BPI23 were prepared successfully , which lay a foundation for later establishment of BPI detecting kit.

Expression of PTEN and FAK in Non-small Cell Lung Cancer and clinical valuation

Ying-hua SONG; Xiao-yan LIN; Yu LI

2009, 29(10): 1102-1103.

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ERK promotes the expression of Smad7 of aorta rat vascular smooth muscle cells

Hua ZHONG; Fang HE; Qing-hua HU; Xiong-ying CHEN; Feng-mei DENG; Zhi-ping SUN; Zeng-chun LI

2009, 29(10): 1104-1105.

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A direct extraction method of protein in cartilage from spine endplate

Ruo-feng YIN; Yi-peng WANG; Zhi-hong WU; Yu ZHAO; Gui-xing QIU

2009, 29(10): 1106-1107.

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Effect of Th1/Th2 cytokine imbalance on the secretion of nerve growth factor in splenic lymphocytes in asthmatic rats

Ruo-yun OUYANG; Cheng-ping HU; Ping CHEN; Jin-qi ZHU; Xin-gang HUANG

2009, 29(10): 1108-1109.

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Anisodamine increase expression of aquaporin1 and 5 in lung during acute lung injury in rats

Shi-sheng WANG; Chun-song YAN; Wei-hua RAO; Jiu-long KUANG

2009, 29(10): 1110-1112.

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miRNAs in cardiac remodeling and heart failure

Chang-xin WANG; Xiao-jian WANG; Ru-tai HUI

2009, 29(10): 1113-1116.

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Heart Failure is a leading cause of mortality for most kinds of cardiovascular diseases. Cardiac remodeling is the milestone of heart failure. The molecular basis of cardiac remodeling and heart failure is still not clear. Most recently, accumulating evidence suggests that miRNAs are involved in cardiac remodeling and heart failure, regulate the expression of target genes and influence the development and progress of cardiac hypertrophy, cardiac fibrosis and heart failure. In the review, we summarize recent insights into the important role and prospective clinical value of miRNAs in cardiac remodeling and heart failure.

Advance in the study of relationship between vimentin and diseases

Hao-xiang XU; Bao-xi WANG

2009, 29(10): 1117-1120.

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Vimentin is an important member of intermediate filament family in mesenchymal cells. Recently, several researches revealed that the changes of vimentin expression and distribution played important roles in regulating cell growth, migration, apoptosis, signaling, gene expression, as well as cellular integrity. Thus, vimemtin had relationships with some diseases, and this article summarized the progress in this field.

Multilocus microsatellite typing for the domestic strains of Cryptococcus neoformans var.grubii

Jian ZHU; Ying LIU; Ohkusu Etsuko; Mikami Yuzuru

2009, 29(10): 2054-1058.

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Objective: Cryptococcus neoformans var. grubii isolates from 6 different cities in our country using multilocus microsatellite typing (MLMT) method were genotyped to explore the genotypic distribution of the variety. Methods: The DNA of forty-three isolates of Cryptococcus neoformans var. grubii which have been identified was extracted. The DNA fragments covering microsatellite loci CNG1, CNG2 and CNG3 were amplified using PCR, and then were sequenced. The numbers of each motif repeat in 3 microsatellite regions ("TA" repeats for CNG1, "GA" repeats for CNG2, and "CAT" repeats for CNG3) were calculated. According to the repeat numbers of these motif , the MLMT types of 43 strains of Cryptococcus neoformans var. grubii were determined. Results：Out of 43 isolates，the percentage of MLMT-17 was 83.72%. In the clinical and environmental isolates, the percentages of MLMT-17 were 86.67% and 70% , respectively. Two new genotypes MLMT-39 and -40 were found. Conclusion: In China , MLMT-17 of Cryptococcus neoformans var. grubii is prevalent in both clinical and environmental strains, implying the most clinical strains which resulted in cryptococcosis originated from indigenous environmental strains.

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