Table of Content

25 April 2009, Volume 29 Issue 4

Previous Issue    Next Issue

研究论文

The crystallization of neural RNA binding protein Musashi1 RRM1

Xian-ping WANG; Li ZHAO; Jing-xi ZHU;

2009, 29(4):  337-341. 

Asbtract ( 382 )   PDF (703KB) ( 604 )  

Related Articles | Metrics

Objection To clarify the structure of important function domain of Musashi1-RRM1, crystallized the Musashi1 RRM1 domain. Mothods The Musashi1 RRM1 was chosen to be expressed, in the E.coli BL21(DE3), purified by ion exchange and gel filtration, and crystal screen used the Hampton Research Kit and optimize the crystallization condition. Results The initial crystal of Musashi1 RRM1 was obtained at last. Conclusion The crystallization of Musashi1 RRM1 is satisfied the following data collection demand.

MEF2C regulates DOK5 gene expression

Jian-yan WEN; Ying-zi ZHANG; Jian-wei YE; Wen-qiang LIAO; Chang-an YU; Yuan-nan KE

2009, 29(4):  342-346. 

Asbtract ( 430 )   PDF (806KB) ( 581 )  

Related Articles | Metrics

Objective To explore the effects of myocyte enhancer factor 2C (MEF2C) on regulation of DOK5 during cardiomyocyte differentiation. Methods RT-PCR was carried out to detect the tissue distribution of mouse DOK5 gene and the expression level of DOK5 and MEF2C during P19CL6 cells differentiated into cardiomyocytes. The effect of MEF2C on DOK5 promoter was confirmed by co-transfection studies. Results The expression of DOK5 was rich in brain, muscle and heart. The mRNA level of DOK5 and MEF2C was enhanced during P19CL6 cells differentiation into cardiomyocytes (p<0.05). Over-expression of MEF2C could increase the promoter activity of DOK5. Furthermore, site-directed mutagenesis demonstrated that MEF2C was crucial for activating DOK5 promoter. Conclusion MEF2C enhanced the transcription of DOK5 through the MEF2 binding site on DOK5 promoter.

Optic nerve transaction combined with high intraocular pressure increased the protein expression levels of p75NTR and sortilin in retina of rats

2009, 29(4):  347-351. 

Asbtract ( 308 )   PDF (759KB) ( 633 )  

Related Articles | Metrics

Objective To observe the changes in sortilin and p75NTR protein expressions in retina of rats following optic nerve transaction combined with high intraocular pressure. Methods Sixty healthy male Wistar rats, were randomly divided into 4 groups: the control (control, n=6), sham (n=6), optic nerve transaction (NT, n=24), and NT combined with high intraocular pressure (NP, n=24) groups. In addition, the NT and NP groups were subdivided into 1, 3, 7 and 14 days' group (n=6 for each group). The p75NTR and sortilin protein expression levels were determined by the quantitative analysis of Western blot. Results The protein expressions of p75NTR, and sortilin increased significantly (p<0.05) in retina of rats from NP group at day 3, 7 and 14 when compared with control, sham and NT groups. Conclusion The increased p75NTR and sortilin protein expressions in retina of rats following high intraocular pressure came from retinal RGC themselves.These results also suggested that the enhanced sortilin (90ku) and p75NTR (60/50ku) protein expressions might be involved in the high ocular pressure-induced retinal RGC injuries of rats.

Upregulation of caspase-3 expression and apoptosis of cell line MG63 induced by pSilencer-VEGF-siRNA transfection

You-shui GAO; Li-zhi ZHANG; Guang QIAN; Tian-lang TONG; Mu HU; Jiong MEI; Xuan-song CAI

2009, 29(4):  352-355. 

Asbtract ( 341 )   PDF (528KB) ( 581 )  

Related Articles | Metrics

Objective To research effects on caspase-3 expression of osteosarcoma cell line MG63 induced by pSilencer-VEGF-siRNA transfection. Methods The cell line MG63 was cultured and human-specific pSilencer-VEGF -siRNA was constructed. The experiment was divided into pSilencer-VEGF-siRNA transfection and vector group. Apoptosis and cell cycle of MG63 was detected and analysesed with conjugated annexin-V (Annexin-V-FITC) antibody and propidium iodide (PI) 48~72 hours after transfection with flow cytometry, and the expression of caspase-3 was detected through Western blotting. Results The early apoptosis of MG63 could be achieved after transfection and with a blockage of G1 phase and cell accumulation occurring. Caspase-3 expression was up-regulated apparently in the transfection group compared with vector group. Conclusion Early apoptosis of MG63 could be induced by pSilencer-VEGF-siRNA, and up-regulation of caspase-3 expression could be achieved.

Distribution and Expression of PTEN Protein of Fibrotic Liver Tissues in Rats

Li-sen HAO; Xiao-lan ZHANG; Xiao-peng TIAN; Jun-yan AN; Na LIU

2009, 29(4):  356-359. 

Asbtract ( 370 )   PDF (605KB) ( 570 )  

Related Articles | Metrics

Objective To explore the role of expression and distribution of PTEN protein in fibrotic liver tissue in the pathological process of hepatic fibrosis in rats. Methods The rat model of hepatic fibrosis was established by common bile duct ligation. The expressions and distribution of PTEN and α-SMA in rat liver tissues were detected by immunohistochemical staining, and expression and distribution of PTEN protein in activated hepatic stellate cell (HSC) in rat liver tissues was assayed by immunofluorescence double labeling confocal laser scanning microscopy. Results With the development of hepatic fibrosis, α-SMA positive cells in rat liver tissues increased significantly (P<0.01), while the expression of PTEN protein decreased gradually (P<0.01). PTEN protein expressed extensively in activated HSC in rat liver tissues, especially in the cytoplasm, and with the aggravation of hepatic fibrosis, the PTEN-expressing activated HSC accounted for an increasingly smaller percentage of total activated HSC (P<0.01). Conclusions The PTEN positive cells in rat liver tissues decreased, its dynamic expression has a significant negative correlation with the activation and proliferation of HSC in vivo.

Desert Hedgehog induces the differentiation of neural progenitor cells from embryonic mesencephalon of rat into dopaminergic neurons

Cai-xia YANG; Huan-ying ZHAO; Chun-li ZHAO; Qian LIU; Qun-yuan XU; Fu-lu GAO

2009, 29(4):  360-365. 

Asbtract ( 300 )   PDF (893KB) ( 548 )  

Related Articles | Metrics

Objective In order to investigate the effect of Desert Hedgehog on direct differentiation of neural progenitor cells (NPCs) cultured from embryonic mesencephalon in the rats. Methods We infected DHH into COS7, NIH/3T3 and 9L cells, and detected the expression of DHH in the cells with immunofluorescence, Real- Time PCR and Western blot. All of the three cells were co-cultured with the NPCs isolated from the ventral mesencephalon in embryonic SD rats (E13.5), respectively. The detection of immunoreactivities of tyrosine hydroxylase (TH) was made by immunocytochemistry after 10 days in vitro. Result The expression of DHH in COS7, NIH/3T3 and 9L cells was remarkably detected, but little TH-positive cells could be found in the three co-cultral systems at the 10th day. Conclusion The protein derived of DHH itself does not show any inductive impact on.the differentiation of NPCs to the dopaminergic neurons in vitro.

Expression of osteolytic cytokines stimulated by micrometer-diameter wear particles

Ming CHEN; Qi-rong DONG; Yu LEI

2009, 29(4):  366-370. 

Asbtract ( 291 )   PDF (814KB) ( 599 )  

Related Articles | Metrics

Objective: To evaluate the influence of the expression of osteolytic cytokines induced by micrometer-diameter wear particles (Ti-6Al-4V and UHMWPE). Methods: Filtration air was injected subcutaneously into rats'back 6 times ( 3ml qod ). After a week, wear particles suspension (Group A: Ti-6Al-4V, Group B: UHMWPE) or physiological saline(Group C) was injected into air pouch tissues. After 14 days, pouch tissues were obtained from killed rats, and were weighted, wax embedded and stained with hematoxylin and eosin, observed under microscope. AKP of serium with Automated Biochemical Analyzer, IL-6 and TNF-α expression with immunohistochemical method, and mRNA expression of extracellular matrix metalloproteinase inducer (EMMPRIN) with real-time Polymerase Chain Reaction method were detected. Results: Air pouch tissues were similar to limiting membrane of periprothesis tissue in the cases of aseptic loosening. As to pouch tissue weight, there was a significant increase in group B than in group C (p<0.05). Microscopy illustrated a large amount of phagocytes in group A and B. The activities of AKP, expression of IL-6 and mRNA expression of EMMPRIN in group A and group B were higher than in group C (P<0.05). Conclusions: The two wear particles both can modulate the production and expression of osteolytic cytokines, lead to aseptic loosening.

Implantation of Canine Tissue Engineering Small －Diameter Blood Vessel

Chao-ji ZHANG; Zhen-zong DU; Guo-tao MA; Zhi-jun HAN; Hua REN; Qi MIAO;

2009, 29(4):  371-374. 

Asbtract ( 320 )   PDF (619KB) ( 522 )  

Related Articles | Metrics

Objective To study the possibility of small diameter TEBV implantation to artery by surgical operation. Methods The mixture of seed cells and scaffold were produced by separately seeding vascular smooth muscle-like cells and vascular endothelium-like cells derived from bone marrow mesenchymal stem cells on PGA\collagen scaffold. The cells scaffold mixture was implanted subcutaneously in cell donor dogs for 8 weeks.The TEBV grafts were used to replace a segment of femoral artery. Results In the group of TEBVs grafts, the grafts were patent and covered with endothelium- like cells, and there was no thrombus formation in the vessels. Histological analysis of the tissue engineering blood vessel wall also revealed a typical artery structure, including endothelium, media and adventitia. Two weeks after implantation, the labeled seed cells by Brdu were found in the three layers of the vessel wall too. Conclusion the tissue engineering vessel could be used to replace natural arteries, the architecture and function of the mixture resembles those of the natural arteries.

Effect of FADDDEL-GFP modification in the islet cell transplantion

Ping HU; Sha WU; Xiao-lan LI; Jing WEI

2009, 29(4):  375-378. 

Asbtract ( 287 )   PDF (516KB) ( 640 )  

Related Articles | Metrics

Objective To study the effect of fusion protein FADDDEL-GFP in the treatment of type 1 diabetes by islet cell transplantion. Methods After transfecting the recombinate FADDDEL-GFP into mouse insulinoma cells NIT-1, insulin level secreted by the cells was detected. Type 1 diabetes model was induced by STZ. NIT-fg cells, the NIT-1 cells modified by FADDDEL-GFP, were transplanted into the diabetic mice. Then the effects of islet cell transplantation on diabetic mice were detected. Results GFP was expressed in NIT-1 cells transfected with FADDDEL-GFP. Diabetic mice reached normoglycemia and prolonged the life span, which is different obviously from the control group. ConclusionsFADDDEL-GFP could enhance the capability of resisting allogeneic transplantation rejection.

Delayed preconditioning reduced apoptosis of myocardial cells in rats

Ying LIU; Yong-mei NIE; Wei-kang WU

2009, 29(4):  379-382. 

Asbtract ( 331 )   PDF (455KB) ( 588 )  

Related Articles | Metrics

Objective Investigate the relation between decrease of apoptosis caused by delayed preconditioning and expression of SMAC and XIAP. Methods Sprage－Dawleyt rats were divided into four groups: control, sham, I/R and IPC/SWOP. The rats in I/R group underwent ischemia for 1 hour by classic artery ligation and reperfusion for 1 hour. The rats in IPC/SWOP group underwent tree cycles of 5-minute ischemia and 5-minute reperfusion 24 hours prior to the index occlusion. Cell apoptosis was measured by flow cytometry, the activity of caspase-3 was also measured. The expression of SMAC and XIAP in cytosol of myocardial cell was analyzed by Western blot. Results Cell apoptosis rate, activity of caspase-3 and expression of SMAC increased significantly in I/R compared with control (p<0.01). The expression of XIAP decreased in I/R compared with control (p<0.01). In IPC/SWOP, cell apoptosis rate, activity of caspase-3 and expression of SMAC decreased significantly compared with I/R (p<0.01). The expression of XIAP increased in IPC/SWOP compared with I/R (p<0.01). Conclusion Myocardial ischemia delayed preconditioning can suppress apoptosis caused by MI/R, the mechanism is related to inhibition of SMAC release from Mitochondria, and in turn maintaining the inhibition of caspase family by XIAP.

Effects of Aβ25-35 on the expression of gene P21、CDK4、E2F1、BAX in PC12 cell

Zhao-yang XIE; Qi-feng ZHU; Bin-hua WU

2009, 29(4):  383-387. 

Asbtract ( 328 )   PDF (616KB) ( 594 )  

Related Articles | Metrics

Objective To study the effects of Aβ25-35 on the expression of gene P21，CDK4，E2F1 and BAX in the cultured PC12 cells. Methods PC12 cells were treated with 25?mol/L Aβ25-35，the relation between cell cycle redistribution and apoptosis was analyzed by flow cytometry（FCM）.Protein and mRNA expression of P21，CDK4，E2F1 and BAX were detected by RT-PCR and Western-blot respectively. Results About 90% PC12 cells were found arrest on G0/G1 by FCM being deprived serum. Treated with 25?mol/L Aβ25-35 for 8，16，24h，the percent of S phase cells was raised remarkably (P<0.01) at 8h, but the percent of S phase cells was declined gradually after treated for 16h. Meanwhile apoptotic rate was increased obviously between 16 h and 24 h(P＜0.01), the hypodiploid peak could be observed ahead of G0/G1 phase(Ap).After synchronized PC12 cells being treated with 25?mol/L Aβ25-35 from 0 to 20h, the mRNA and protein expression of P21 gene were downregulated, while CDK4，E2F1 and BAX gene were upregulated. Conclusion Aβ25-35 induces the synchronized PC12 cells attempting to re-enter cell cycle，which appear the apoptotic peak subsequently. This is likely related to decreasing the mRNA and protein expression of P21 gene, and increasing the mRNA and protein expression of CDK4，E2F1 and BAX gene.

Differentiation of marrow bone mesenchymal stem cells into hepatocytes from rat in vitro

Zhong-qiong WANG; Chang-ping LI; Shi-ping XIA; Yan LIU; Xiao-lin ZHONG

2009, 29(4):  388-392. 

Asbtract ( 294 )   PDF (933KB) ( 608 )  

Related Articles | Metrics

Objectives To study the potential of hepatocyte growth factor (HGF), acid fibroblast growth factor (aFGF) and oncostatin M (OSM) to induce MSCs differentiate into hepatocytes and their roles. Methods MSCs were treated with HGF, aFGF and OSM. Detecting the expression of AFP, ALB and urea was performed by chemi-luminescent technique, RT-PCR method and glutamic acid dehydrogenase technique. Results AFP expression emerged in every group except control group (P<0.05). ALB and urea expression emerged in other groups except control group and a+O group (P<0.05). Conclusion HGF, OSM and aFGF can induce MSCs differentiate into hepatocytes with the expression of AFP, ALB and excreting urea, while aFGF and OSM can not induce MSCs differentiate into mature hepatocyte without HGF.

Genetic Analysis of α-ADDUCIN and GNB3 in Essential Hypertension Patient

Hong-ye ZHAO; Jun CAO; Li ZHOU; Bin WANG; Chang-chun QIU

2009, 29(4):  393-396. 

Asbtract ( 375 )   PDF (377KB) ( 665 )  

Related Articles | Metrics

Objective To explore the polymorphism at position G460W of α-ADDUCIN and at position C825T of GNB3 and the genetic interaction between α-ADDUCIN and GNB3 genes in a QiQihr essential hypetension population. Methods Three hundreds and thirty-one patients with EH and two hundreds and ninety-three healthy controls were selected．Genotyping was performed using PCR-RFLP technique. Results (1)genotype distributions of alpha- ADDUCIN G460W (GG 0.177 vs 0.160, GW 0.580 vs 0.481, WW 0.242 vs 0.359, P=0.006) and GNB3 (CC0.177 vs 0.353, CT 0.468 vs 0.541, TT 0.355 vs 0.106, P＜0.001) between NT group and EH group; there were significantly differences in W allele frequency of alpha-ADDUCIN G460W ( 0.532 vs 0.600, P=0.017) between two group. (2) GNB3 (CC0.177 vs 0.353, CT0.468 vs 0.541, TT0.355 vs 0.106, P＜0.001) between NT group and EH group.; there were significantly different in T allele frequency of GNB3 C825T (0.589 vs 0.376, P＜0.001) between two group; (3)The genotype distributions of WW+CC and WW+CT of the EH group were significantly higher than the NT group (P value respectively ＜0.001 and＜0.01), yet the genotypes distribution of GG+TT、GW+TT and WW+TT of the EH group were significantly lower than the NT group (P value respectively ＜0.01、＜0.001 and ＜0.001). Conclusion These observations suggested that alpha-ADDUCIN G460W and GNB3 C825T polymorphisms are associated with essential hypertension in QiQihr population, and there might be an interacting effect between the two genes.

Metastasis correlation factors expression and NF-κB activation in colorectal carcinoma

Kun YU; Mei HAN; Jin-kun WEN; Bing-hui LI

2009, 29(4):  397-399. 

Asbtract ( 310 )   PDF (339KB) ( 547 )  

Related Articles | Metrics

Objective To investigate the relationship between NF-κB activation and the expression of metastasis correlation factors. Methods The expression of NF-κB，COX-2, ICAM-1, VCAM-1 and MMP-9 in cancer and normal tissues was detected using Western blotting. Results 1. The expression level of NF-κB was higher in cancer tissues than that in normal tissues (P<0.05). 2. The expression of COX-2, ICAM-1 and VCAM-1 was higher in 75% cancer tissues, in which NF-κB was high expression. 3. MMP-9 expression was negatively correlated with its profilin SM22α. Conclusion The expression of metastasis correlation factors was positively related with the activation of NF-κB, and was responsible for the progression of colorectal carcinoma and poor prognosis.

Antisense oligonecleotides of CT120 inhibited the growth of lung adenocarcinoma cell line A549

Zun-ling LI; Shu-hong SHAO; Shu-yang XIE; Fei JIAO; Shou-seng SHI; Xue-yan YU

2009, 29(4):  400-404. 

Asbtract ( 449 )   PDF (730KB) ( 609 )  

Related Articles | Metrics

Object To clarify the relationship between CT120, a novel human plasma membrane-associated gene, and prolifetation of lung adenocarcinoma cell line A549. Method Expression vector（pcDNA3.1）containing antisense oligonucleotides of CT120 was constructed and transfected into the lung adenocarcinoma cell line A549. RT-PCR and Western blot detected the expression of CT120. Meantime, flow cytometry and soft agarose colony formation analyzed the cell proliferation, and P53、CYCLIND1 and CDK4 were detected by RT-PCR. Result pcDNA3.1 containing antisense oligonucleotides of CT120 was constructed successfully and could inhibit the expression of CT120 effectively by RT-PCR and Western blot. The expression of P53 was up-regulated and the expression of CYCLIND1、CDK4 were down-regulated. Conclusion The down-regulation of CT120 expression by antisense oligonucleotides technique may be a new candidate of drug target for treatment of lung cancer.

Effect of the exogenous fragile histidine triad (FHIT)gene on proliferation and apoptosis of cutaneous carcinoma cell line A431

Xiang-feng SONG; Zhong-wei TIAN; Dan-dan FU; Xin-ling BI

2009, 29(4):  405-408. 

Asbtract ( 352 )   PDF (552KB) ( 537 )  

Related Articles | Metrics

Objective To investigate the effect of the exogenous fragile hisdidine triad (FHIT) gene on the proliferation and the apoptosis of cutaneous carcinoma cell line A431, and to search for the mechanism of tumor suppression by the FHIT gene. Methods By the method of liposome transfection, plasmids pcDNA3-FHIT and pcDNA3-vector were transfected into the cutaneous carcinoma cell line A431 which was absent of FHIT gene expression, and then the transfected cells were screened by G418 and the expression of FHIT was determined by the immunocytochemical staining technique. The effect of FHIT on the growth characteristics of cutaneous carcinoma cell line A431 was observed by MTT, the colony forming test and flow cytometry. Results Stable FHIT gene expressing A431 cells were produced,and the proliferation activity and the colony forming capability of A431-FHIT were suppressed, whereas the apoptosis rate of it was increased. All these differences between A431-FHIT cells and the two control groups of cutaneous carcinoma cells had statistical significance. Conclusions Transfecting the exogenous FHIT gene into cutaneous carcinoma cells line A431can suppress the proliferation of tumor cells,and can also induce apoptosis and cell cycle arrest .

Suppression of VEGF Antisense Oligonucleotide on growth of micrangium of gastric cancer cells xenograft in nude mice

Bin ZHENG; Wen-tao YIN

2009, 29(4):  409-413. 

Asbtract ( 298 )   PDF (635KB) ( 498 )  

Related Articles | Metrics

Objective To observe the effects of VEGF antisense oligonucleotide on growth of micrangium of xenograft in nude mice. Methods: The VEGF-ASODN was synthesised artificially..After transfection with VEGF-ASODN, RNA copies was detected by real-time PT-PCR ,VEGF PROTEIN was detected by ELESA, Survival of cells was detected by MTT.Growth of cells was evaluated by growth curve. After the model of human gastic cancer xenografts in nude mice were founded,they were distributed randomly into three groups:antisense group、scrambled group and saline group. We established control group . Results ：VEGF-ASODN can reduce the VEGFmRNA and VEGF PROTEIN in cells and supernate remarkably . Survival and growth of cells was suppressed. It can reduce VEGF PROTEIN in serum of nude mice significantly . It can also reduce the volum and weight of xenografts.Density of micrangium decreased in xenografts. Conclution VEGF-ASODN can suppressed the growth of micrangium in xenografts.

技术与方法

Preparation , identification and celluler localization of PIWIL proteins

Yu-xia LU; Zhi-gang HUANG; Xi-mei CHEN; Heng-jun GAO; Ying HU; Xiao-yan ZHANG; Xun MENG

2009, 29(4):  414-417. 

Asbtract ( 401 )   PDF (656KB) ( 521 )  

Related Articles | Metrics

Objective To prepare polyclonal antibodies against human PIWIL, identify their property and investigate the tissue distribution of PIWIL. Methods PIWIL polypeptide was synthesized by chemical method, and conjugated to Keyhole limpet hemocyanin (KLH) as immunogen. Then PIWIL-KLH conjugations were injected into rabbits subcutaneously to produce polyclonal antibodies. The specificity and sensitivity of antibodies were identified by ELISA and Western blot after purification using affinity chromatography. PIWIL were then immune stained on the tissue chip to study their distribution. Results Rabbit antibodies against PIWIL were prepared after injection of PIWIL-KLH conjugation. These antibodies were confirmed to specially recognize PIWIL peptides. Expression of PIWIL was found in the cytoplasm of epithelia cells of varied normal tissue and tumor tissue . Conclusions The successful preparation of the polyclonal antibody against PIWIL will provide efficient reagent for further study of its role in the pathway of miRNA and RNA interference and in the pathogenesis of human disease.

A strategy for rapidly constructing the single and two site targeting plasmid-based RNAi vector

Da-peng DAI; Jian-ping CAI

2009, 29(4):  418-422. 

Asbtract ( 348 )   PDF (760KB) ( 524 )  

Related Articles | Metrics

Objective To facilitate rapid construction of RNAi vector. Methods After inversely inserting the U6 and H1 polymerase Ⅲ promoters into the pBluescriptⅡbackbone vector, only need two short reverse complementary oligo nucleotides, the RNAi vector with one interference cassette could be conveniently constructed. Using two isoaudamers MunⅠ and EcoRⅠwhich could generate compatible sticky ends, several cassettes could be readily fused together to produce the ultimate multiple-site targeting RNAi vector. Using this method, we constructed RNAi vectors targeting green fluorescent protein (GFP) and firefly luciferase (LUC) gene separately or both, in order to test the knock down property of these vectors. Results Constructed single and two site targeting RNAi vectors could get desirable knockdown effects, in which single site targeting vector could get about 90 percent knock down efficiency whereas two site targeting vector could get about 87.5 percent knock down efficiency either. Conclusion Our RNAi vector construction strategy is so efficient and time-saving that it can be used for knocking down several genes simultaneously in same cell.

Species identification of animal cells by polymerase chain reaction

Xiao-cui BIAN; Yu-qin LIU; Chun-jing WANG; Xiao-ling SU; Xiao-mei ZHAO; Bei GU; Hong ZHANG

2009, 29(4):  423-430. 

Asbtract ( 304 )   PDF (1532KB) ( 651 )  

Related Articles | Metrics

Objective To identify the species of cultured cells and to detect inter-species cross contamination by polymerase chain reaction (PCR). Methods From references and NCBI database, we commercially synthesized 32 pairs of species-specific primers targeted to genomic DNA of ten common species of cultured cells. Then we screened for optional primers with good specificity and high sensitivity. PCR amplification with these primers and the genomic DNAs isolated from the tested cell line, and agarose gel electrophoresis of the PCR products were done. Mixed DNA of 10 species was used as positive-control template, and water as negative-control template. Results Finally 10 pairs of species-specific and high sensitive primers were selected. By PCR amplification with these primers and agarose gel electrophoresis, we could easily tell the origin of cell lines and detect whether the tested cell lines are contaminated by cells of other species. Conclusions This PCR assay provides a simple, rapid, sensitive, and cost-effective method to identify cell species and detect interspecies cross-contamination.

临床园地

Diagnosis and surgical treatment of intravenous tumor embolus extending through inferior vena cava into the right cardiac cavities

Guo-tao MA; Qi MIAO; Hua REN; Xing-rong LIU; Chao-ji ZHANG; Heng ZHANG; Li-hua CAO

2009, 29(4):  431-435. 

Asbtract ( 365 )   PDF (743KB) ( 645 )  

Related Articles | Metrics

Objective Renal tumor and gynecological tumor invading into inferior vena cava and extending to the right cardiac cavities is rare,we report the experience of diagnosis and surgical treatment of intravenous tumor embolus extending through inferior vena cava into the right cardiac cavities. Methods From Junuary 2001 to May2008 ,4 patients with intravenous tumor embolus extending through inferior vena cava into the right cardiac cavities were treated stagedly in PUMCH .Three patients are leiomyomatosis. Two patients' operations were performed by stages, firstly removal of tumer in the right cardiac cavities was performed using cardiopulmonary bypass under mid - hypothermia, and ,postoperatively 3 - 4weeks, total abdominal hysterectomy with bilateral salpingo- oophorectomy including the tumor mass was performed. The other one patient's tumor were resected at one time.One patient is renal clear cell carcinoma, The urologist performed abdominal nephrectomy and then cardiac surgeon resect tumor embolus using cardiopulmonary bypass under deep hypothermic total circulatory. Results The four patients have uneventful recovery ,there was no death or any serious perioperative complications. Followed up of 3 months to 4 years showed no tumor recurrence after the operation, they are all survival. Conclusion Confirm diagnosis,the tumor embolus extending through inferior vena cava into the right cardiac cavities should be suspected among patients with renal cell carcinoma and multiple hysteromyoma.Successful therapy for intravenous tumor embolus is dependent on total one or two- stage surgical excision of the tumor and multi-department cooperation，and combined therapy.

研究短文

Effects of valproate on dopaminergic neurons and expression of brain-derived neurotrophic factor in Parkinson's disease model mice

Yu-mei ZHANG; Xue-fei WU; De-qin YU; Yan-hui FENG; Wan-qin ZHANG; Jie ZHAO

2009, 29(4):  436-438. 

Asbtract ( 1132 )   PDF (550KB) ( 575 )  

Related Articles | Metrics

Fluvastatin decreased glomerulosclerosis by adriamycin in rats

Hong-xia YUAN; Dong-xiao SUN

2009, 29(4):  439-440. 

Asbtract ( 271 )   PDF (666KB) ( 648 )  

Related Articles | Metrics

医学教育

Research on the factors that affects the employment of postgraduates of basic medicine

Jin-yan FAN; Yun ZHANG; Ruo-fan LI

2009, 29(4):  441-442. 

Asbtract ( 373 )   PDF (252KB) ( 559 )  

Related Articles | Metrics

Currently the employment of students of basic medical science has become a challenge. In this report, some important factors that affect the employment are analyzed, such as the students' qualities, the reputation of school, the opinion to occupation, information and so on. Some responding strategies are proposed such as guaranteeing the students' qualities, strengthening reformation and employment tutoring principles, all those ways help the students to face the situation reasonably and build the right employment opinion to better their choice of occupation.

短篇综述

A review of metabonomics in research of traditional Chinese medicine

Qiao-feng WU; Shu-guang YU; Yong TANG

2009, 29(4):  443-445. 

Asbtract ( 374 )   PDF (329KB) ( 506 )  

Related Articles | Metrics

Metabonomics is a new method which concerns with the relationship between the organism metabolites with the environment and the effect of stimulates from outside. It provides a new strategy and platform for the TCM study and the modernization of TCM since the main idea of metabonomics is in accordance with that of TCM.