Table of Content

05 July 2010, Volume 30 Issue 7

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研究论文

Cloning of Guinea Pig Bile Salt Export Pump (BSEP) and Analyzed its expressions in Cholelithiasis Guinea Pigs liver

Ming-wei HUANG; Qian-li TANG; Jun HE; Yuan YU; Qing-jian WANG; Wei HUANG

2010, 30(7):  673-676. 

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Objective To clone and analyze the full-length cDNA sequence of guinea pigs bile salt export pump gene(BSEP) and detect its expressions in cholelithiasis guinea pigs liver. Methods The BSEP gene full-length cDNA of guinea pigs was amplified by 3'and 5'RACE.Bioinformatic methods were applied to analyz and identify the sequence. Real-time PCR was used to quantification BSEP gene expressions in Cholelithiasis guinea pig liver.Results The length of the guinea pigs BSEP gene cDNA was 5579 bp, containing altogether 28 extrons and a 3963 bp-length open reading frame(ORF) encoding a 1320- amino -acids protein. BSEP mRNA expressions in cholelithiasis guinea pigs liver were significantly decreasses compared to the normal group（P<0.01）. Conclusions Down-regulation of BSEP mRNA expressions in cholelithiasis guinea pigs liver may be relative to the formation of cholelithiasis.

Association of cholesteryl ester transfer protein gene TaqⅠB polymorphism with Essential Hypertension in Chinese Mongolian population

Xiao-ming TAO; Guo-jiang LI; Shu-qin HOU; Zhan-sen XIAO; Wei-jun TONG; Yong-yue LIU; Gang WU; Yong-hong ZHANG; Chang-chun QIU

2010, 30(7):  677-682. 

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Objective To explore the association of cholesteryl ester transfer protein gene TaqⅠB polymorphism with Essential Hypertension in Chinese Mongolian population. Methods A total of 1819 Mongolian people, including 840 hypertensive patients and 979 normal controls from agriculture and pastoral areas in Tongliao city of Inner Mongolia, were included in this case-control study. We identified the genotypes of CETP TaqⅠB with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and direct sequencing technology. Results The frequency distributions of CETP gene TaqⅠB locus genotypes and alleles in men, women, and the whole population, were in accordance with the Hardy-Weinberg equilibrium. The differences of TaqⅠB locus genotypes and alleles frequency distributions between Essential Hypertension and healthy controls were not statistically significant in men, women, and the total population. In the male group, the individuals carrying AA genotype had higher high-density lipoprotein cholesterol level and lower total cholesterol and triglyceride levels, compared to those with GG genotype (P <0.05). Conclusion CETP gene TaqⅠB polymorphism may not be a direct risk factor for essential hypertension, but it can affect the blood lipid levels, might be indirectly involved in the pathogenesis of Essential Hypertension.

Relationship between the APOB gene polymorphism and stroke in China Fanshan district

Kuo CHEN; Zhan-sen XIAO; Hui-dong DOU; Shu-qin HOU; Yue-feng LIU; Xiao-ming TAO; Feng LI; Chang-chun QIU

2010, 30(7):  683-688. 

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Objective To investigate the relationship between polymorphism of the APOB gene and stroke in Han Chinese population．Methods This study was a population-based cross-sectional case-control study. In APOB gene, the polymorphisms of C7673T and 5′I/D were determined by Polymerase Chain Restriction-Restriction Fragment Length Polymorphism (PCR-RFLP) and sequencing in 547 patients with ischemie stroke (466 ischemie stroke) and 886 controls． Results The distributions of C7673T (p=0.6293) and 5′I/D (p=0.5359) genotype frequencies in men and women were in accordance with the Hardy-Weinberg equilibrium. In C7673T locus, the genotype CC frequency in females was significant lower in ischemic stroke than that in healthy controls (p＜0.01), and allele C frequency was significant lower in ischemic stroke than that in healthy controls (p＜0.01). But significant differences were not found in males. The frequency of genotype II at 5′I/D locus in males was significant lower in cerebral hemorrhage patients than that in healthy controls (p＜0.01). The frequency of genotype II and the allele I at 5′I/D locus in males was significant lower in ischemic stroke patients than that in healthy controls（p＜0.01）. The frequency of genotype II and the allele I at 5′I/D locus in females was also significant lower in ischemic stroke patients than that in healthy controls, （p＜0.01，p＜0.05，respectively）Conclusion The C7673T polymorphism of APOB gene associates with ischemic stroke in Fangshan district in female. The 5′I/D polymorphism of APOB gene associates with ischemic stroke in Fangshan district in male and female.

Histone acetyltransferase regulates cell cycle and cell proliferation of mesenchymal stem cells induced by 5-azacytidine

Na ZHOU; Jing ZHU; Jie TIAN; Ya-lan ZHANG; Bing DENG; Ya-sha LI

2010, 30(7):  689-697. 

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Objective The purpose of the experiment is explaining the role of histone acetyltransferase GCN5 in the process of mesenchymal stem cells induced by 5-azacytidine and the regulation mechanism of GCN5 complex. Methods Cell proliferation was evaluated by MTT assay, cell cycle by flow cytometry and expression of P21 by real-time PCR. Then we used co-immunoprecipitation strategy to separate interaction proteins with GCN5 and tandem mass spectrometry to identify interacting proteins. CHIP strategy was used for analyzing binding ability between P21 promoter and GCN5 together with acetylated histones at the GCN5 site on the P21 gene. Results The proportion of G0/G1 phase MSCs and expression of P21 gene reached the highest at 3 days after 5-azaC-induced, then gradually decreasing. Cell proliferation index PI value and the G2/S phase cell ratio were the opposite to the above. GCN5 physically interacted with the following proteins which were categorized as：ATP-dependent chromatin remodeling complex、transcription initiation complex、transcription factor and zinc finger protein. Binding-abilities and levels of acetylation of 5-azaC treated group were higher than that of untreated group. Conclusion High concentration of 5-azaC increases G0/G1 phase arrest for MSCs. G0/G1 phase arrest decreased and cell proliferation increased as the concentration of 5-azaC decreasing. 5-azaC raised the combination of GCN5-protein-complexes and the P21 gene promoter in vitro differentiation of MSCs through regulating G0/G1 phase of cell cycle and cell proliferation.

Antiproliferative and antiangiogenic effect of rapamycin on ADPKD cystic lining epithelial cells

Chun-yan LIU; Chang-lin MEI; Li YUAN; Yi ZHANG; Li-li FU; Hou-an CAI; Xue-qi WANG

2010, 30(7):  698-702. 

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Objective To investigate antiproliferative effect and reduction of VEGF expression of rapamycin on ADPKD cystic lining epithelial cells. Methods ADPKD cystic lining immortalized epithelial cells(WT9-12) were stimulated by rapamycin with different concentrations. After treatment, MTT method was performed to detect the levels of proliferation, the mRNA expressions of VEGF were measured by Real-time PCR. Western blot method was used to detect the protein expressions of cyclinD, P21, Bcl2, Bax and VEGF. Results Rapamycin , a mTOR inhibitor, was found to induce cell growth inhibition, apoptosis as well as a stable accumulation of cells in the G0/G1 phase. Western blot of cultured WT9-12 cells demonstrated that Rapamycin downregulated cyclinD, Bcl2 and upregulated p21, Bax expressions. In addition, realtime-PCR detected elevated levels of VEGF mRNA in primary cells from ADPKD patients and WT9-12 cells compared with normal tubule cells. Rapamycin downregulated VEGF expression in WT9-12 cells. Conclusion Rapamycin induces ADPKD cystic lining epithelial cells growth inhibition, apoptosis and inhibits VEGF expression, which may be one of mechanisms of rapamycin postponing ADPKD progression.

Comparison of PDZK1 and PDZK2 and their binding properties

Yuan LI; Si-qi HU; Su-can MA; Chen SHAO; Yi MU; You-he GAO

2010, 30(7):  703-707. 

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Objective compare PDZs from two homologous simple adaptor proteins PDZK1 and PDZK2 and their binding properties. Method we used yeast two-hybrid screening of a random peptide library and high throughput validation screening of a specialized PDZ ligand candidate library to systematically and comprehensively identify PDZ ligands, consensus-binding sequences were deduced from sequence alignment of positive clones. Two PDZs were compared by structure-guided sequence alignment. The binding properties of the PDZ domains were analyzed. Result the C-terminal consensus sequence for binding to PDZK1-PDZ1 and PDZK2-PDZ1 are -[S/T]-x-Φ and -[S/T]-[K/R/H]-[F/L] respectively. The contacting residues on the PDZ domains are similar, only the amino acids between 37 and 45 are difference, especially at the 42 position (F vs H). Conclusion Homologous PDZK1 and PDZK2 have different contacting residues and different binding properties.

The Interaction Between PLC-β3 and Syntrophin

Yan HUANG; Ying XIONG; Hua LIU; Chao-yuan SUN; Yang LI; Jun-qi HE

2010, 30(7):  708-713. 

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Objective To identify the interaction between PLC-β3 and the PDZ domain-containing protein syntrophin, providing clues to the underlying roles of PLC-β3 in signaling pathway of the nervous system. Methods The carboxyl-terminal of PLC-β3 (PLC-β3-CT) was fused to GST protein by gene cloning. The resultant protein was used for examining interactions of PLC-β3-CT with the PDZ-domain of β2-syntrophin and γ2-syntrophin respectively by GST pull-down assay. Results PLC-β3-CT cDNA was cloned into the expression vector pGEX-4T-1 successfully, and the GST fusion protein was overexpressed and purified in E.coli system. GST pull-down experiments revealed that the PDZ domain of β2-syntrophin, but not γ2-syntrophin was robustly pulled down by the carboxyl-terminal of PLC-β3. Conclusion These results provide a strong evidence for further studying the intracellular association of the PLC-β3 and β2-syntrophin and helping in exploring their potential physiological significances in signaling pathway of the nervous system.

Upregulation of p27Kip1 expression by SIRT1 in rat vascular smooth muscle cells

Yun-biao LU; Hou-zao CHEN; Ran ZHANG; Wei ZHENG; Li LI; Qing-jun ZHANG; De-pei LIU

2010, 30(7):  714-720. 

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Objective To explore the impact of SIRT1 on p27Kip1 expression in vascular smooth muscle cells (VSMCs ) and investigate the possible mechanism. Methods A7r5 VSMCs were treated wtih H2O2 or oxLDL,then SIRT1 expression was detected by Western blot. Vascular injury models were also used to detect SIRT1 expression by Western blot. Primary rat VSMCs were infected with wild-type SIRT1 adenovirues and examined the expression of p27 by Western blot. Then we treated quiescent cells with nicotinamide, an inhibitor of the deacetylase activity of SIRT1, then detected changes of p27 expression. We also detected the interaction between SIRT1 and FOXO3a in VSMCs by co-immunoprecipitation. Results Endogenous SIRT1 was upregulated in a time-dependent manner in H2O2 or oxLDL treated A7r5 cells, and also upregulated in vascular injury models. SIRT1 overexpression significantly upregulated p27 expression under 10%FBS stimulation. NAM was found to inhibit p27 expression in a dose-dependent manner. The interaction between SIRT1 and FOXO3a was detectable in smooth muscle cells. Conclusion SIRT1 upregulates p27 expression in vascular smooth muscle cells.

Prokaryotic expression, purification and crystallization of human PHF8

Shuo HUANG; Lin YU; Yang WANG; Yin LIU; Zhong-zhou CHEN

2010, 30(7):  721-725. 

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Objective Purification and crystallization of human PHF8. Methods The plasmid PHF8/pet28b, constructed by molecular cloning, was transformed into Rosetta2 (DE3) cell, and PHF8 was induced by IPTG. Recombinant protein was purified, crystallized, and diffracted and its structure was solved. Result Recombinant PHF8 protein was expressed in E. coli, and subjected to affinity purification followed by gel filtration separation. PHF8 protein with hexagon-1ike crystals was obtained. Conclusion The enzymatically active PHF8 protein was purified using the prokaryotic expression system, its crystal particle was obtained, and the overall structure of PHF8 catalytic core was resolved.

Location of the receptor for Netrin-4 by primary culture of neurons of newborn rat

Ke-ke YU; Si-ying WANG; Ren-ping ZHOU; Cheng-gang ZHANG

2010, 30(7):  726-730. 

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Objective To establish a primary culture model of neurons of newborn rat and investigate whether the known Netrin-1 receptors, DCC and UNC5H1, are co-receptors for Netrin-4. Method We improved Koh method to cultivate the primary neurons of newborn rat. Evaluation of neurons was made by immunocytochemical techniques. Ligand-receptor binding assays and in situ staining with the alkaline phosphatase (AP4) tagged Netrin-4 fusion protein were used. Reverse transcription polymerase chain reaction (RT-PCR) method was also used to observe the expression level of Netrin-4, DCC, UNC5H1 mRNA in brain during development. Result Cultured neurons were stick to the wall of the cultivation plate when they were growing. The neurons were satiety and with long axon. Immunocytochmical staining showed 90% neurofilament positive staining cell. In situ staining of AP4-Netrin-4 protein bound to COS7 cells transiently transfected with eukaryotic expression coding DCC and UNC5H1 showed that the Netrin-4 could bind to the transfected cells. Conclusion Primary culture of olfactory and cortical neurons of newborn rat by the method is a reliable and high stable way to obtain neurons. Primary culture of olfactory and cortical neurons of newborn rat by the method is a reliable and high stable way to obtain neurons.DCC and UNC5H1 are coreceptors for Netrin-4

The expression of MMP-9 and ICAM-1 in rats with cerebral ischemic /reperfusion treated by acupuncture

Hong XU; Li-chuan HONG; Yan-qiu HUANG; Su-hui CHEN; Hua SUN

2010, 30(7):  731-736. 

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To study the cerebral expression of MMP-9 and ICAM-1 in rats with acupuncture after cerebral ischemic /reperfusion. Methods SD rats were randomly divided into seven groups, electro-acupuncture at "Zusanli" and "Bai hui " group , "Baihui" group, Nimodipime group , joint treatment group of acupuncture and medicine, model ,sham group and control group. Each group contains 12、24、48、72、96 and 144h six time points. The pathological lesion was detected by H.E. staining. We explored the expression of MMP-9 and ICAM-1 in core infarct area by Immunohistochemistry staining. Results H.E. staining showed that the pathological lesion reached the peak at 48th hour in the infarct core area; until the 72th hour cerebral interstitial oedema was alleviated. Also many glial cells and collagen fibers proliferate, and new blood capillaries appear. MMP-9 and ICAM-1 was widely expressed in vascular endothelial and inflammatory cells together with some neurons by immunohistochemistry staining. The positive cells in the ipsilateral region is more than in contralateral region; Comparison within groups shows MMP-9 and ICAM-1 expression at 48th and 144th hours exhibited a double-peak profile, while the expression in electro- acupuncture group at 48h、72 and 96h time points has significant difference compared with that in model group. Conclusions: acupuncture may have the effects on promoting neurological function recovery by up-regulating injury mediation network.

Construction and migration of rat bone marrow mesenchymal stem cells coexpressing EGFP and CXCR4

Xiao-lan YU; Zhi-jian ZHANG; Xiu-li WU; Zhi-xin HUANG

2010, 30(7):  737-742. 

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Objective To construct rat mesenchymal stem cells (rMSCs) coexpressing CXCR4 and EGFP and observe the effect of CXCR4 on rMSCs migration. Methods The total RNA was isolated from SD rat's liver with Trizol, the CXCR4 gene was amplified by RT-PCR. The CXCR4 was inserted into the transfer vector of lentivirus after the vector was digested with restriction endonuclease. The lentiviral particles were produced in 293T cells by transient cotransfection involving a three-plasmid expression system, then were harvested and concentrated. The rMSCs were transfected by obtained lentiviral particles or null lentiviral particles. The expressing of CXCR4 gene in CXCR4-rMSCs or null-rMSCs was evaluated with RT-PCR, Western blotting, cellular immunofluorescence and flow cytometry. The migration ability of transfected cells induced by SDF-1 was assessed in the transwell system. Results The results showed that the full-length fragment of CXCR4 gene was successfully cloned into the transfer vector of lentivirus. The result of sequencing showed that the cloned CXCR4 gene was consistent with that reported in GenBank. The CXCR4 gene-modified rMSCs could express both the CXCR4 and EGFP. The expression of CXCR4 gene in the CXCR4-rMSCs group (experimental group) was significantly higher than that in the null-rMSCs group (control group) at both mRNA and protein levels. The migration ratios of the CXCR4-rMSCs group was significantly higher than that of the null-rMSCs group. Conclusion The rMSCs coexpressing CXCR4 and EGFP was successfully constructed, this will greatly facilitate further research, such as the evaluation of the effect of SDF-1/CXCR4 on rMSCs' recruitment to damaged tissue.

Induction of endothelial progenitor cell from peripheral blood

Wei-qi LI; Lei ZHENG; Qian WANG; Ya-juan SU; Zhen CAI

2010, 30(7):  743-748. 

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Objective To investigate induction of endothelial progenitor cell（EPC）from peripheral blood. Methods Peripheral blood mononuclear cells （PBMCs） were isolated from healthy volunteers using density gradient centrifugation. They were suspended with EGM-2 medium and cultured in 6-well plate which was precoated with fibronectin. During cultivation, adherent cells were kept on observing and then examined by immunofluorescence staining for characteristic while flow cytometry for cell cycle and surface markers such as CD34、CD133、CD31、VEGFR2 and CD14. Results Adherent cells turned slenderness gradually, accounting for 4.62%~5.47% of PBMCs. Almost all of them were stayed at G0/G1 stage, with a proliferation index about 1.20%±0.18%. They began to uptake acLDL and exhibit lectin binding capability simultaneously from the fifth day and then form enough Ⅷ factor related antigen on day 9. The expression of EPC related surface markers including CD34、CD133、CD31、VEGFR2 、CD14 were 0.19%±0.06%、1.67%±0.52%、61.56%±5.57% 70.29%±7.37% and 89.31%±4.11% respectively. All these indicated that adhesion cells were differentiating EPC. Conclusion Under specific condition, it is possible to induce EPC from PBMCs directly.

Reconstruction of If Channel in HCN4 Transfected Mesenchymal Stem Cells in Rats

Min TONG; Xiang-jun YANG; Lian-hua HAN; Hong-xia LI; Xin ZHAO; Ya-feng ZHOU; Wen-ping JIANG

2010, 30(7):  749-753. 

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Objective To reconstruct the If channel by transfection of hHCN4 gene in rat mesenchymal stem cells (rMSCs). Methods Bone marrow was collected from SD rats by flushing femars and tibias with DMEM. The third passage cells were used for experiments. The identification of MSCs was carried out by flow cytometry when immune reaction with CD45 and CD90 antibodies. The Lentivirus vector Lenti-GFP-HCN4 was constructed by plasmid pRsv-REV、pMDlg-pRRE、pMD2G and pCDH1-GFP-HCN4 in 293 cells. After transduced MSCs with Lentivs vectors, RT-PCR and immunofluorescence were used to detect the expression of mRNA and protein of hHCN4 channel. Finally recorded the If in the positively transfected cells with whole-cell voltage clamp. Results The transfection efficiency for MSCs was about 60%. There was hHCN4 mRNA in MSCs transfected with HCN4. The recorded hyperpolarization-activated inward current from positive MSCs was time- and voltage-dependent. The activated threshold voltage was about -80mV. The current was sensitive to CsCl. The control cells didn't show the current at the same clamp voltage. Conclusion The functional If channels can be reconstructed in rMSCs overexpression with hHCN4.

Polymorphism of the CETP gene in the healthy population of Hainan Li and Han nationality

Dai-feng ZHOU; Wang-wei CAI; Mei-ling YUN; Yun-xia ZHANG; Yong ZHANG; Jun-li XI; Zhen WANG

2010, 30(7):  754-758. 

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Objective To understand the frequency of the 6 Polymorphism sites in the CETP gene in the healthy population of Hainan Li and Han nationality: TaqIB、I405V、D442G、R451Q、A373P and I14A .Method We detected the mutations of the 6 Polymorphism sites in the CETP gene in the healthy population of Hainan Li and Han nationality by the special allele specific-PCR system and analyzed the data by the statistics method. Result 3 genotypes can be detected according to TaqIB Polymorphism site in both Hainan Han and Li humanity which is B1B1，B1B2 and B2B2.3 genotypes can also be detected according to I405V Polymorphism site in both Hainan Han and Li humanity which is II,IV and VV. 2 genotypes can be detected according to D442G Polymorphism site in both Hainan Han and Li humanity which is DD and DG .Mutation of R451Q、A373P and I14A3 can't be detected both in Han and Li humanity. Genotype frequency（P<0.0001） and allele frequency（P<0.0001） of polymorphism site I405V exist significant difference between Hainan Han and Li humanity，while the other polymorphism sites exist no significant difference. Conclusion The frequent mutation sites of CETP gene in Hainan Han and Li humanity is TaqIB、I405V and D442G, of which I405V exist significant differenc, while the mutations in R451Q、A373P and I14A are infrequent.

Prevalence and Determinants of hypertension in middle-aged adults of Beijing

Bo HU; Wei LI; Bing LIU; Tao CHEN; Yi SUN

2010, 30(7):  759-762. 

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Objective The present study aimed to determine the prevalence of hypertension and try to find the risk factors in the urban and rural area of Beijing. Methods A cluster sampling was used to establish a study population of inhabitants aged 40 to 60. 3268 middle-aged participants were enrolled in the investigation. The definition of hypertension was determined by National Revision Committee of the Guidelines of Hypertension Prevention and Control in 2005. Logistic regression model was used to find the risk factors of hypertension. Results In total population of this study, the prevalence in urban area was significantly lower than in rural area (55.58% vs 42.91%, P<0.05). In urban area, the prevalence of hypertension in male and female were 49.54% and 38.52% which were significantly lower than the prevalence in rural area 57.68% and 54.17%.(all P<0.05). Multivariate logistic analysis revealed that age, BMI, waist, high density lipoprotein cholesterol, fasting blood glucose and education were the risk factors of hypertension in male, which, age, BMI, waist, occupation and triglyceride were the risk factors of hypertension in female. Conclusion The data indicated that the prevalence of hypertension in rural area maybe have higher than in urban in developed area of our country. The most important risk factors are BMI and waist.

Association of IL-10 Gene Polymorphism with Atopic Dermatitis

Yin-ji XU; Jin-ping TONG; Hui-juan WANG

2010, 30(7):  763-766. 

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Objective To study the association of the interleukin-10(IL-10) haplotype polymorphism and the Atopic Dermatitis. Methods 174 Atopic Dermatitis children and 130 healthy children were tested, The frequency of three single nucleotide polymorphisms(SNPs) at positions-1082(A/G), -819(C/T), -592(A/C) and corresponding haplotypes in the promoter region of the IL-10 gene were analysed using Taqman method. The data were assessed for correlations with the eosinophil count and total serum IgE concentration. Results The frequency of IL-10 haplotype ACC in healthy children was higher than that in Atopic Dermatitis children (P﹤0.05) . Meanwhile medians of serum IgE level and total eosinophil count in Atopic Dermatitis children with ATA/ATA genotype were higher than those with ATA/ACC (P﹤0.05) . Conclusion IL-10 promotor polymorphism may be associated with the development of Atopic Dermatitis.

临床园地

Risk factors for adult Henoch-Schonlein purpura nephritis with gastrointestinal manifestations

Long FANG; Chun GAO; Hong-chuan ZHAO

2010, 30(7):  767-770. 

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Objective Our study was designed to determine the risk factors for Henoch-Schonlein purpura nephritis (HSPN) in adult patients with gastrointestinal manifestations. Methods The demographic, clinical, laboratorial, pathologic features and prognosis of 118 adult HSPN patients, who treated at the China-Japan Friendship Hospital, Ministry of Health, during the period of January 1, 2004 to May 31, 2009, were analyzed. Results Of the 118 patients, 52 cases (44.1%) were diagnosed with gastrointestinal manifestations (case group) and 66 were without gastrointestinal manifestations (control group). Compared with the control group, patients in the case group have higher percentage of joint involvement (P<0.001), higher neutrophil count (P<0.05), higher platelet count (P<0.01), lower albumin (P<0.05), lower serum natrium (P<0.05) and lower immunoglobulin G (P<0.05). In unconditional Logistic analysis, joint involvement (OR 5.010), neutrophil count (OR 1.248), and immunoglobulin G (OR 0.999) showed statistical difference. Conclusion Higher percentage of joint involvement, higher neutrophil count and lower immunoglobulin G are three independent risk factors for HSPN in adult patients with gastrointestinal manifestations.

研究短文

Application of liquid-based cytology technique to preoperative detection of estrogen and progesterone receptor in breast cancer

Xi-bo LIU; Ai-jing SUN; Ning WANG; Li-ping SUN; Li-rong CHEN

2010, 30(7):  771-772. 

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Increasing expression of nitrative stress in hepatic tissue of rats with alcoholic liver disease

Jun-ying ZHOU; Lin WANG; Wei WANG

2010, 30(7):  773-774. 

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短篇综述

Myocytes apoptosis signal transduction and the myocardium injury

Ri-xin DAI; Lang LI

2010, 30(7):  775-777. 

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The cardiac myocyte apoptosis signal transduction are major include caspase-dependend pathways and caspase-independend pathway ,and also involve crosstalk or interaction with the inflammation、oxidation stress、autophagy and other mechanisms signal networks in myocardium injury. The apoptotic signaling pathway determine the live or death of cardiac myocyte in the heart. It has a great influence on the development and prognosis of the myocardium injury.

Angiotensin-converting enzyme 2 as a new therapeutic target for cardiovascular fibrosis

Wei WU; Jiu-chang ZHONG; Jun-ming GUO

2010, 30(7):  778-781. 

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Angiotensin-converting enzyme 2 (ACE2) is the first human homologue of ACE, being a new therapeutic target for cardiovascular fibrosis and remodeling. This review focuses on the biological features of ACE2, ACE2 and its modulation in cardiovascular fibrosis as well as the latest advance in prevention and treatment of cardiovascular fibrosis.

The association between estrogen and thyroid carcinoma

Lu ZHANG; Yue-wu LIU

2010, 30(7):  782-784. 

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Estrogen exclusively influences the growth of thyroid carcinoma cells by estrogen receptors. Estrogen binds estrogen response element by estrogen receptor, then activating many genes ，these genes involve the growth of thyroid carcinoma cells . And estrogen stimulates the growth of thyroid carcinoma cells by the mitogen activated protein kinases signal transduction pathway.