Knockdown of PIAS3 alleviates glucose fluctuation-induced oxidative stress and mitochondrial dysfunction in rat cardiomyocyte cell line H9c2

doi:10.16352/j.issn.1001-6325.2025.12.1593

Abstract

Abstract: Objective To investigate the effect of PIAS3 on glucose fluctuation-induced oxidative stress and mitochondrial dysfunction in rat cardiomyocytes. Methods H9c2 were cultured in vitro, and divided into normal glucose control group(Control), mannitol-induced osmotic pressure control group(MG), constant high glucose group(HG), intermittent hyperglycemia group(IHG), IHG+siRNA NC group, and IHG+PIAS3 siRNA group. Cell proliferation was assessed using CCK-8 assay. LDH release,MDA and GSH levels, as well as SOD activity, were detected using corresponding kits. Mitochondrial membrane potential was evaluated via JC-1 staining combined with flow cytometry. ROS levels in cells and mitochondria were determined using DCFH-DA and MitoSOX staining, respectively. Protein expression of PI3K, p-PI3K, AKT, and p-AKT was analyzed by Western blot. Results Compared with the control group, intermittent hyperglycemia promoted oxidative stress and mitochondrial dysfunction, significantly upregulated PIAS3 expression(P＜0.001) and downregulated p-PI3K and p-AKT protein levels(P＜0.001). Knockdown of PIAS3 significantly alleviated oxidative stress and mitochondrial dysfunction induced by glucose fluctuations, and increased p-PI3K and p-AKT protein levels(P＜0.001). Conclusions Knockdown of PIAS3 may alleviate glucose fluctuation-induced oxidative stress and mitochondrial dysfunction in ratcardiomyocytes by activating the PI3K/AKT signaling pathway.

Key words: PIAS3, glucose fluctuation, oxidative stress, mitochondrial dysfunction

CLC Number:

R692
R587.2

CHENG Yongxia, YU Long, LI Huamin, ZHAO Shuo, ZHANG Yiyang, LIU Guibo. Knockdown of PIAS3 alleviates glucose fluctuation-induced oxidative stress and mitochondrial dysfunction in rat cardiomyocyte cell line H9c2[J]. Basic & Clinical Medicine, 2025, 45(12): 1593-1599.

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